Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing
Recently, lichens came once more into the scientific spotlight due to their unique relations with prokaryotes. Several temperate region lichen species have been thoroughly explored in this regard yet, the information on Antarctic lichens and their associated bacteriobiomes is somewhat lacking. In th...
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ftpubmed:oai:pubmedcentral.nih.gov:10373906 2023-08-20T04:02:26+02:00 Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing Woltyńska, Aleksandra Gawor, Jan Olech, Maria A Górniak, Dorota Grzesiak, Jakub 2023-02-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373906/ http://www.ncbi.nlm.nih.gov/pubmed/36750176 https://doi.org/10.1093/femsec/fiad015 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373906/ http://www.ncbi.nlm.nih.gov/pubmed/36750176 http://dx.doi.org/10.1093/femsec/fiad015 © The Author(s) 2023. Published by Oxford University Press on behalf of FEMS. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. FEMS Microbiol Ecol Research Article Text 2023 ftpubmed https://doi.org/10.1093/femsec/fiad015 2023-07-30T01:11:08Z Recently, lichens came once more into the scientific spotlight due to their unique relations with prokaryotes. Several temperate region lichen species have been thoroughly explored in this regard yet, the information on Antarctic lichens and their associated bacteriobiomes is somewhat lacking. In this paper, we assessed the phylogenetic structure of the whole and active fractions of bacterial communities housed by Antarctic lichens growing in different environmental conditions by targeted 16S rRNA gene amplicon sequencing. Bacterial communities associated with lichens procured from a nitrogen enriched site were very distinct from the communities isolated from lichens of a nitrogen depleted site. The former were characterized by substantial contributions of Bacteroidetes phylum members and the elusive Armatimonadetes. At the nutrient-poor site the lichen-associated bacteriobiome structure was unique for each lichen species, with chlorolichens being occupied largely by Proteobacteria. Lichen species with a pronounced discrepancy in diversity between the whole and active fractions of their bacterial communities had the widest ecological amplitude, hinting that the nonactive part of the community is a reservoir of latent stress coping mechanisms. This is the first investigation to make use of targeted metatranscriptomics to infer the bacterial biodiversity in Antarctic lichens. Text Antarc* Antarctic PubMed Central (PMC) Antarctic FEMS Microbiology Ecology 99 3 |
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Research Article Woltyńska, Aleksandra Gawor, Jan Olech, Maria A Górniak, Dorota Grzesiak, Jakub Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
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Research Article |
description |
Recently, lichens came once more into the scientific spotlight due to their unique relations with prokaryotes. Several temperate region lichen species have been thoroughly explored in this regard yet, the information on Antarctic lichens and their associated bacteriobiomes is somewhat lacking. In this paper, we assessed the phylogenetic structure of the whole and active fractions of bacterial communities housed by Antarctic lichens growing in different environmental conditions by targeted 16S rRNA gene amplicon sequencing. Bacterial communities associated with lichens procured from a nitrogen enriched site were very distinct from the communities isolated from lichens of a nitrogen depleted site. The former were characterized by substantial contributions of Bacteroidetes phylum members and the elusive Armatimonadetes. At the nutrient-poor site the lichen-associated bacteriobiome structure was unique for each lichen species, with chlorolichens being occupied largely by Proteobacteria. Lichen species with a pronounced discrepancy in diversity between the whole and active fractions of their bacterial communities had the widest ecological amplitude, hinting that the nonactive part of the community is a reservoir of latent stress coping mechanisms. This is the first investigation to make use of targeted metatranscriptomics to infer the bacterial biodiversity in Antarctic lichens. |
format |
Text |
author |
Woltyńska, Aleksandra Gawor, Jan Olech, Maria A Górniak, Dorota Grzesiak, Jakub |
author_facet |
Woltyńska, Aleksandra Gawor, Jan Olech, Maria A Górniak, Dorota Grzesiak, Jakub |
author_sort |
Woltyńska, Aleksandra |
title |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_short |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_full |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_fullStr |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_full_unstemmed |
Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing |
title_sort |
bacterial communities of antarctic lichens explored by gdna and cdna 16s rrna gene amplicon sequencing |
publisher |
Oxford University Press |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373906/ http://www.ncbi.nlm.nih.gov/pubmed/36750176 https://doi.org/10.1093/femsec/fiad015 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
FEMS Microbiol Ecol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373906/ http://www.ncbi.nlm.nih.gov/pubmed/36750176 http://dx.doi.org/10.1093/femsec/fiad015 |
op_rights |
© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_doi |
https://doi.org/10.1093/femsec/fiad015 |
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FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
3 |
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1774712884516880384 |