Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia
BACKGROUND: Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu l...
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ftpubmed:oai:pubmedcentral.nih.gov:10369706 2023-08-20T04:07:59+02:00 Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia Miljević, Milan Rajičić, Marija Umhang, Gérald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena 2023-07-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369706/ http://www.ncbi.nlm.nih.gov/pubmed/37491284 https://doi.org/10.1186/s13071-023-05879-x en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369706/ http://www.ncbi.nlm.nih.gov/pubmed/37491284 http://dx.doi.org/10.1186/s13071-023-05879-x © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Parasit Vectors Research Text 2023 ftpubmed https://doi.org/10.1186/s13071-023-05879-x 2023-07-30T01:02:35Z BACKGROUND: Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu lato (s.l.) species is difficult without the use of molecular methods. The aim of this study was to identify and explore the genetic diversity of Hydatigera and other taeniid species. METHODS: Ten different small mammals species (856 individuals) (Rattus rattus, three Apodemus, three Arvicolinae and three Soricidae species) were examined from 2013 to 2023. Captured animals were visually examined for cysts and visible lesions. Two markers were used for amplification and sequencing: cox1 and 12S rDNA. RESULTS: Molecular analysis of cysts and visible lesions revealed four taeniid species: Hydatigera kamiyai, H. taeniaeformis sensu stricto (s.s.), Taenia martis and T. crassiceps. Hydatigera kamiyai was found in Apodemus flavicollis, A. agrarius, Microtus arvalis and Crocidrua leucodon, while H. taeniaeformis s.s. is registered in R. rattus. Hydatigera kamiyai cox1 sequences clustered with European populations and showed at least 25 nucleotid differences compared to Asian, African, Australian and one of our isolates of H. taeniaeformis s.s acquired from a rat, followed by large sequence distances (9.4% to 12.9%), indicating clear molecular distinction of two species. CONCLUSIONS: This is one of the few mitochondrial gene-based studies performed after the description of cryptic entities within the Hydatigera taeniaeformis s.l. complex and represents a valuable contribution to understanding of genetic diversity, host suitability and geographic distribution of these tapeworm species. Also, our study provides an important basis of molecular data from this part of Europe for further studies. We emphasize the importance of additional studies of intermediate hosts, especially rats from Europe and Apodemus spp. and voles from Asia ... Text Microtus arvalis Rattus rattus PubMed Central (PMC) Parasites & Vectors 16 1 |
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Research Miljević, Milan Rajičić, Marija Umhang, Gérald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
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Research |
description |
BACKGROUND: Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu lato (s.l.) species is difficult without the use of molecular methods. The aim of this study was to identify and explore the genetic diversity of Hydatigera and other taeniid species. METHODS: Ten different small mammals species (856 individuals) (Rattus rattus, three Apodemus, three Arvicolinae and three Soricidae species) were examined from 2013 to 2023. Captured animals were visually examined for cysts and visible lesions. Two markers were used for amplification and sequencing: cox1 and 12S rDNA. RESULTS: Molecular analysis of cysts and visible lesions revealed four taeniid species: Hydatigera kamiyai, H. taeniaeformis sensu stricto (s.s.), Taenia martis and T. crassiceps. Hydatigera kamiyai was found in Apodemus flavicollis, A. agrarius, Microtus arvalis and Crocidrua leucodon, while H. taeniaeformis s.s. is registered in R. rattus. Hydatigera kamiyai cox1 sequences clustered with European populations and showed at least 25 nucleotid differences compared to Asian, African, Australian and one of our isolates of H. taeniaeformis s.s acquired from a rat, followed by large sequence distances (9.4% to 12.9%), indicating clear molecular distinction of two species. CONCLUSIONS: This is one of the few mitochondrial gene-based studies performed after the description of cryptic entities within the Hydatigera taeniaeformis s.l. complex and represents a valuable contribution to understanding of genetic diversity, host suitability and geographic distribution of these tapeworm species. Also, our study provides an important basis of molecular data from this part of Europe for further studies. We emphasize the importance of additional studies of intermediate hosts, especially rats from Europe and Apodemus spp. and voles from Asia ... |
format |
Text |
author |
Miljević, Milan Rajičić, Marija Umhang, Gérald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena |
author_facet |
Miljević, Milan Rajičić, Marija Umhang, Gérald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena |
author_sort |
Miljević, Milan |
title |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_short |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_full |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_fullStr |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_full_unstemmed |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_sort |
cryptic species hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in serbia |
publisher |
BioMed Central |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369706/ http://www.ncbi.nlm.nih.gov/pubmed/37491284 https://doi.org/10.1186/s13071-023-05879-x |
genre |
Microtus arvalis Rattus rattus |
genre_facet |
Microtus arvalis Rattus rattus |
op_source |
Parasit Vectors |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369706/ http://www.ncbi.nlm.nih.gov/pubmed/37491284 http://dx.doi.org/10.1186/s13071-023-05879-x |
op_rights |
© The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
op_doi |
https://doi.org/10.1186/s13071-023-05879-x |
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