Species Identification of Caviar Based on Multiple DNA Barcoding
This study aimed to explore the applicability of DNA barcoding for assessing the authenticity of caviar on the Chinese market. A set of universal COI primers and two sets of designed primers based on COI and D-loop genes were used to identify maternal species of samples from 21 batches of caviar. Th...
Published in: | Molecules |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
MDPI
2023
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343275/ https://doi.org/10.3390/molecules28135046 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:10343275 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:10343275 2023-07-30T03:55:24+02:00 Species Identification of Caviar Based on Multiple DNA Barcoding Hu, Qingqing Pan, Yingqiu Xia, Huili Yu, Kexin Yao, Yian Guan, Feng 2023-06-28 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343275/ https://doi.org/10.3390/molecules28135046 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343275/ http://dx.doi.org/10.3390/molecules28135046 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Molecules Article Text 2023 ftpubmed https://doi.org/10.3390/molecules28135046 2023-07-16T01:20:15Z This study aimed to explore the applicability of DNA barcoding for assessing the authenticity of caviar on the Chinese market. A set of universal COI primers and two sets of designed primers based on COI and D-loop genes were used to identify maternal species of samples from 21 batches of caviar. The results showed that the PCR products from three sets of primers had more than 98% similarity to the sequences in database. The COI gene could not distinguish sturgeons with closed genetic relationships, while D-loop gene could effectively improve the accuracy of DNA barcoding and was more suitable to the identification of interspecific sturgeon than the COI gene. The neighbor-joining dendrogram further confirmed the applicability and accuracy of COI and D-loop genes in identifying maternal relatives of caviar (Acipenser baerii/Acipenser gueldenstaedtii/Acipenser schrenckii/Huso dauricus/Huso huso). Despite the limitations of mitochondrial DNA in identifying hybrid sturgeon species, the presence of counterfeit caviar of non-sturgeon ingredients could be excluded. All the caviar samples were identified successfully as sturgeon species, but the mislabeling rate of species was 33.4%, indicating that there were illegal phenomena such as disorderly labeling, mislabeling, and adulteration on the market. Text Acipenser baerii PubMed Central (PMC) Molecules 28 13 5046 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Hu, Qingqing Pan, Yingqiu Xia, Huili Yu, Kexin Yao, Yian Guan, Feng Species Identification of Caviar Based on Multiple DNA Barcoding |
topic_facet |
Article |
description |
This study aimed to explore the applicability of DNA barcoding for assessing the authenticity of caviar on the Chinese market. A set of universal COI primers and two sets of designed primers based on COI and D-loop genes were used to identify maternal species of samples from 21 batches of caviar. The results showed that the PCR products from three sets of primers had more than 98% similarity to the sequences in database. The COI gene could not distinguish sturgeons with closed genetic relationships, while D-loop gene could effectively improve the accuracy of DNA barcoding and was more suitable to the identification of interspecific sturgeon than the COI gene. The neighbor-joining dendrogram further confirmed the applicability and accuracy of COI and D-loop genes in identifying maternal relatives of caviar (Acipenser baerii/Acipenser gueldenstaedtii/Acipenser schrenckii/Huso dauricus/Huso huso). Despite the limitations of mitochondrial DNA in identifying hybrid sturgeon species, the presence of counterfeit caviar of non-sturgeon ingredients could be excluded. All the caviar samples were identified successfully as sturgeon species, but the mislabeling rate of species was 33.4%, indicating that there were illegal phenomena such as disorderly labeling, mislabeling, and adulteration on the market. |
format |
Text |
author |
Hu, Qingqing Pan, Yingqiu Xia, Huili Yu, Kexin Yao, Yian Guan, Feng |
author_facet |
Hu, Qingqing Pan, Yingqiu Xia, Huili Yu, Kexin Yao, Yian Guan, Feng |
author_sort |
Hu, Qingqing |
title |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_short |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_full |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_fullStr |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_full_unstemmed |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_sort |
species identification of caviar based on multiple dna barcoding |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343275/ https://doi.org/10.3390/molecules28135046 |
genre |
Acipenser baerii |
genre_facet |
Acipenser baerii |
op_source |
Molecules |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10343275/ http://dx.doi.org/10.3390/molecules28135046 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_doi |
https://doi.org/10.3390/molecules28135046 |
container_title |
Molecules |
container_volume |
28 |
container_issue |
13 |
container_start_page |
5046 |
_version_ |
1772810725679431680 |