Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection
This paper outlines methods used to identify novel antigens for use in the development of serological assays. Specifically, we applied these methods to a neurogenic parasitic nematode of cervids called Parelaphostrongylus tenuis. This parasite is of particular concern in both wild and domestic ungul...
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ftpubmed:oai:pubmedcentral.nih.gov:10329008 2023-07-30T03:55:46+02:00 Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection Richards, Jessie Kania, Stephen Wilson, Abigail Kent, Emily Gerhold, Richard 2023-07-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329008/ http://www.ncbi.nlm.nih.gov/pubmed/37419916 https://doi.org/10.1038/s41598-023-37481-7 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329008/ http://www.ncbi.nlm.nih.gov/pubmed/37419916 http://dx.doi.org/10.1038/s41598-023-37481-7 © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . Sci Rep Article Text 2023 ftpubmed https://doi.org/10.1038/s41598-023-37481-7 2023-07-16T00:42:40Z This paper outlines methods used to identify novel antigens for use in the development of serological assays. Specifically, we applied these methods to a neurogenic parasitic nematode of cervids called Parelaphostrongylus tenuis. This parasite is of particular concern in both wild and domestic ungulates as it causes significant neurological signs and definitive diagnosis is only possible post-mortem, necessitating the development of serologic assays for antemortem diagnosis. Proteins extracted from P. tenuis organisms were affinity isolated using antibodies enriched from seropositive moose (Alces alces). The proteins were analyzed using mass spectrometry and liquid chromatography to obtain amino acid sequences that were then cross-referenced to open reading frames predicted from an assembled transcriptome. An antigen of interest was assessed for immunogenic epitopes and subsequently synthesized into 10-mer synthetic overlapping peptides representing these regions. These synthetic peptides were then assessed for reactivity against positive and negative moose sera and demonstrated potential use as a serological assay in diagnostic laboratories. Known negative moose sera revealed significantly lower optical density when compared to the positive samples (p < 0.05). This method serves as a pipeline for the construction of diagnostic assays of pathogens in both human and veterinary medicine. Text Alces alces PubMed Central (PMC) Scientific Reports 13 1 |
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Article Richards, Jessie Kania, Stephen Wilson, Abigail Kent, Emily Gerhold, Richard Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection |
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Article |
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This paper outlines methods used to identify novel antigens for use in the development of serological assays. Specifically, we applied these methods to a neurogenic parasitic nematode of cervids called Parelaphostrongylus tenuis. This parasite is of particular concern in both wild and domestic ungulates as it causes significant neurological signs and definitive diagnosis is only possible post-mortem, necessitating the development of serologic assays for antemortem diagnosis. Proteins extracted from P. tenuis organisms were affinity isolated using antibodies enriched from seropositive moose (Alces alces). The proteins were analyzed using mass spectrometry and liquid chromatography to obtain amino acid sequences that were then cross-referenced to open reading frames predicted from an assembled transcriptome. An antigen of interest was assessed for immunogenic epitopes and subsequently synthesized into 10-mer synthetic overlapping peptides representing these regions. These synthetic peptides were then assessed for reactivity against positive and negative moose sera and demonstrated potential use as a serological assay in diagnostic laboratories. Known negative moose sera revealed significantly lower optical density when compared to the positive samples (p < 0.05). This method serves as a pipeline for the construction of diagnostic assays of pathogens in both human and veterinary medicine. |
format |
Text |
author |
Richards, Jessie Kania, Stephen Wilson, Abigail Kent, Emily Gerhold, Richard |
author_facet |
Richards, Jessie Kania, Stephen Wilson, Abigail Kent, Emily Gerhold, Richard |
author_sort |
Richards, Jessie |
title |
Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection |
title_short |
Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection |
title_full |
Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection |
title_fullStr |
Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection |
title_full_unstemmed |
Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection |
title_sort |
novel methods of immunogenic antigen selection for serological diagnosis of parelaphostrongylus tenuis infection |
publisher |
Nature Publishing Group UK |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329008/ http://www.ncbi.nlm.nih.gov/pubmed/37419916 https://doi.org/10.1038/s41598-023-37481-7 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329008/ http://www.ncbi.nlm.nih.gov/pubmed/37419916 http://dx.doi.org/10.1038/s41598-023-37481-7 |
op_rights |
© The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_doi |
https://doi.org/10.1038/s41598-023-37481-7 |
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