Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities
Microbial activity in Arctic soils controls the cycling of significant stores of organic carbon and nutrients. We studied in situ processes in Alaskan soils using original metaproteomic methods in order to relate important heterotrophic functions to microbial taxa and to understand the microbial res...
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ftpubmed:oai:pubmedcentral.nih.gov:10308928 2023-07-23T04:16:48+02:00 Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities Miller, Samuel E. Colman, Albert S. Waldbauer, Jacob R. 2023-06-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308928/ http://www.ncbi.nlm.nih.gov/pubmed/37272710 https://doi.org/10.1128/msystems.01238-22 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308928/ http://www.ncbi.nlm.nih.gov/pubmed/37272710 http://dx.doi.org/10.1128/msystems.01238-22 Copyright © 2023 Miller et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . mSystems Research Article Text 2023 ftpubmed https://doi.org/10.1128/msystems.01238-22 2023-07-02T01:34:01Z Microbial activity in Arctic soils controls the cycling of significant stores of organic carbon and nutrients. We studied in situ processes in Alaskan soils using original metaproteomic methods in order to relate important heterotrophic functions to microbial taxa and to understand the microbial response to Arctic greening. Major bacterial groups show strong metabolic specialization in organic topsoils. α-/β-/γ-Proteobacteria specialized in the acquisition of small, soluble compounds, whereas Acidobacteria, Actinobacteria, and other detritosphere groups specialized in the degradation of plant-derived polymers. α-/β-/γ-Proteobacteria dominated the expression of transporters for common root exudates and limiting nitrogenous compounds, supporting an ecological model of dependence upon plants for carbon and competition with plants for nitrogen. Detritosphere groups specialized in distinct substrates, with Acidobacteria producing the most enzymes for hemicellulose depolymerization. Acidobacteria was the most active group across the three plant ecotypes sampled—the largely nonvascular, lower biomass intertussock and the largely vascular, higher biomass tussock and shrub. Functional partitioning among bacterial groups was stable between plant ecotypes, but certain functions associated with α-/β-/γ-Proteobacteria were more strongly expressed in higher biomass ecotypes. We show that refined metaproteomic approaches can elucidate soil microbial ecology as well as biogeochemical trajectories of major carbon stocks. IMPORTANCE: The Arctic is warming twice as fast as the rest of the planet, and Arctic soils currently store twice as much carbon as the entire atmosphere—two facts that make understanding how Arctic soil microbial communities are responding to climate change particularly urgent. Greening of vegetation cover across the Arctic landscape is one of the most prominent climate-driven shifts in Arctic terrestrial ecology, with potentially profound effects on biogeochemical cycling by the soil microbiome. Here we use ... Text Arctic Greening Arctic Climate change permafrost PubMed Central (PMC) Arctic mSystems 8 3 |
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Research Article Miller, Samuel E. Colman, Albert S. Waldbauer, Jacob R. Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
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Research Article |
description |
Microbial activity in Arctic soils controls the cycling of significant stores of organic carbon and nutrients. We studied in situ processes in Alaskan soils using original metaproteomic methods in order to relate important heterotrophic functions to microbial taxa and to understand the microbial response to Arctic greening. Major bacterial groups show strong metabolic specialization in organic topsoils. α-/β-/γ-Proteobacteria specialized in the acquisition of small, soluble compounds, whereas Acidobacteria, Actinobacteria, and other detritosphere groups specialized in the degradation of plant-derived polymers. α-/β-/γ-Proteobacteria dominated the expression of transporters for common root exudates and limiting nitrogenous compounds, supporting an ecological model of dependence upon plants for carbon and competition with plants for nitrogen. Detritosphere groups specialized in distinct substrates, with Acidobacteria producing the most enzymes for hemicellulose depolymerization. Acidobacteria was the most active group across the three plant ecotypes sampled—the largely nonvascular, lower biomass intertussock and the largely vascular, higher biomass tussock and shrub. Functional partitioning among bacterial groups was stable between plant ecotypes, but certain functions associated with α-/β-/γ-Proteobacteria were more strongly expressed in higher biomass ecotypes. We show that refined metaproteomic approaches can elucidate soil microbial ecology as well as biogeochemical trajectories of major carbon stocks. IMPORTANCE: The Arctic is warming twice as fast as the rest of the planet, and Arctic soils currently store twice as much carbon as the entire atmosphere—two facts that make understanding how Arctic soil microbial communities are responding to climate change particularly urgent. Greening of vegetation cover across the Arctic landscape is one of the most prominent climate-driven shifts in Arctic terrestrial ecology, with potentially profound effects on biogeochemical cycling by the soil microbiome. Here we use ... |
format |
Text |
author |
Miller, Samuel E. Colman, Albert S. Waldbauer, Jacob R. |
author_facet |
Miller, Samuel E. Colman, Albert S. Waldbauer, Jacob R. |
author_sort |
Miller, Samuel E. |
title |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_short |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_full |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_fullStr |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_full_unstemmed |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_sort |
metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected arctic soil bacterial communities |
publisher |
American Society for Microbiology |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308928/ http://www.ncbi.nlm.nih.gov/pubmed/37272710 https://doi.org/10.1128/msystems.01238-22 |
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Arctic |
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Arctic |
genre |
Arctic Greening Arctic Climate change permafrost |
genre_facet |
Arctic Greening Arctic Climate change permafrost |
op_source |
mSystems |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308928/ http://www.ncbi.nlm.nih.gov/pubmed/37272710 http://dx.doi.org/10.1128/msystems.01238-22 |
op_rights |
Copyright © 2023 Miller et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
op_doi |
https://doi.org/10.1128/msystems.01238-22 |
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