Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan
Cold-adapted or psychrotrophic fermentative anaerobic bacteria were isolated from rice field soil in a temperate area in Japan using anaerobic enrichment cultures incubated at 5°C. Most isolates were obligately anaerobic, spore-forming rods and affiliated with different lineages of the genus Clostri...
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Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
2023
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308237/ http://www.ncbi.nlm.nih.gov/pubmed/37164688 https://doi.org/10.1264/jsme2.ME22109 |
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ftpubmed:oai:pubmedcentral.nih.gov:10308237 2023-07-23T04:21:22+02:00 Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan Honma, Sachi Ueki, Atsuko Ichimura, Akio Suzuki, Kouki Kaku, Nobuo Ueki, Katsuji 2023 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308237/ http://www.ncbi.nlm.nih.gov/pubmed/37164688 https://doi.org/10.1264/jsme2.ME22109 en eng Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308237/ http://www.ncbi.nlm.nih.gov/pubmed/37164688 http://dx.doi.org/10.1264/jsme2.ME22109 2023 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Microbes Environ Regular Paper Text 2023 ftpubmed https://doi.org/10.1264/jsme2.ME22109 2023-07-02T01:32:50Z Cold-adapted or psychrotrophic fermentative anaerobic bacteria were isolated from rice field soil in a temperate area in Japan using anaerobic enrichment cultures incubated at 5°C. Most isolates were obligately anaerobic, spore-forming rods and affiliated with different lineages of the genus Clostridium based on 16S rRNA gene sequences. The growth temperature ranges and physiological properties of three representative clostridial isolates (C5S7, C5S11(T), and C5S18) were examined. Strain C5S7 grew at 0°C, but not at 20°C, and was identified as Clostridium estertheticum, a psychrophile isolated from spoiled, vacuum-packed, chilled meat (blown pack spoilage, BPS). Strain C5S7 produced butyrate, n-butanol, and abundant gases (H(2) and CO(2)) as major fermentation products from the carbohydrates utilized. Strain C5S11(T), which was recently described as Clostridium gelidum sp. nov., possessed psychrotrophic properties and grew at temperatures between 0 and 25°C. Strain C5S11(T) was saccharolytic, decomposed polysaccharides, such as inulin, pectin, and xylan, and produced acetate, butyrate, and gases. Strain C5S18 also grew at 0°C and the optimum growth temperature was 15°C. Strain C5S18 did not ferment carbohydrates and grew in a manner that was dependent on proteinaceous substrates. This strain was identified as the psychrotolerant species, Clostridium tagluense, originally isolated from a permafrost sample. Collectively, the present results indicate that psychrotrophic anaerobic bacteria with different physiological properties actively degrade organic matter in rice field soil, even in midwinter, in a cooperative manner using different substrates. Furthermore, different psychrotrophic species of the genus Clostridium with the ability to cause BPS inhabit cultivated soil in Japan. Text permafrost PubMed Central (PMC) Midwinter ENVELOPE(139.931,139.931,-66.690,-66.690) Microbes and Environments 38 2 n/a |
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Regular Paper |
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Regular Paper Honma, Sachi Ueki, Atsuko Ichimura, Akio Suzuki, Kouki Kaku, Nobuo Ueki, Katsuji Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan |
topic_facet |
Regular Paper |
description |
Cold-adapted or psychrotrophic fermentative anaerobic bacteria were isolated from rice field soil in a temperate area in Japan using anaerobic enrichment cultures incubated at 5°C. Most isolates were obligately anaerobic, spore-forming rods and affiliated with different lineages of the genus Clostridium based on 16S rRNA gene sequences. The growth temperature ranges and physiological properties of three representative clostridial isolates (C5S7, C5S11(T), and C5S18) were examined. Strain C5S7 grew at 0°C, but not at 20°C, and was identified as Clostridium estertheticum, a psychrophile isolated from spoiled, vacuum-packed, chilled meat (blown pack spoilage, BPS). Strain C5S7 produced butyrate, n-butanol, and abundant gases (H(2) and CO(2)) as major fermentation products from the carbohydrates utilized. Strain C5S11(T), which was recently described as Clostridium gelidum sp. nov., possessed psychrotrophic properties and grew at temperatures between 0 and 25°C. Strain C5S11(T) was saccharolytic, decomposed polysaccharides, such as inulin, pectin, and xylan, and produced acetate, butyrate, and gases. Strain C5S18 also grew at 0°C and the optimum growth temperature was 15°C. Strain C5S18 did not ferment carbohydrates and grew in a manner that was dependent on proteinaceous substrates. This strain was identified as the psychrotolerant species, Clostridium tagluense, originally isolated from a permafrost sample. Collectively, the present results indicate that psychrotrophic anaerobic bacteria with different physiological properties actively degrade organic matter in rice field soil, even in midwinter, in a cooperative manner using different substrates. Furthermore, different psychrotrophic species of the genus Clostridium with the ability to cause BPS inhabit cultivated soil in Japan. |
format |
Text |
author |
Honma, Sachi Ueki, Atsuko Ichimura, Akio Suzuki, Kouki Kaku, Nobuo Ueki, Katsuji |
author_facet |
Honma, Sachi Ueki, Atsuko Ichimura, Akio Suzuki, Kouki Kaku, Nobuo Ueki, Katsuji |
author_sort |
Honma, Sachi |
title |
Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan |
title_short |
Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan |
title_full |
Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan |
title_fullStr |
Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan |
title_full_unstemmed |
Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan |
title_sort |
phylogeny and physiological diversity of cold-adapted anaerobic bacteria isolated from rice field soil in japan |
publisher |
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308237/ http://www.ncbi.nlm.nih.gov/pubmed/37164688 https://doi.org/10.1264/jsme2.ME22109 |
long_lat |
ENVELOPE(139.931,139.931,-66.690,-66.690) |
geographic |
Midwinter |
geographic_facet |
Midwinter |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
Microbes Environ |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308237/ http://www.ncbi.nlm.nih.gov/pubmed/37164688 http://dx.doi.org/10.1264/jsme2.ME22109 |
op_rights |
2023 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_doi |
https://doi.org/10.1264/jsme2.ME22109 |
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Microbes and Environments |
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38 |
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2 |
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1772186847021105152 |