Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods
Green extraction methods using a combination of mechanical, enzymatic, and green chemical treatments were evaluated for the sequential extraction of carotenoid pigments, protein, and chitin from crab processing discards. Key objectives included avoiding the use of hazardous chemical solvents, conduc...
Published in: | Marine Drugs |
---|---|
Main Authors: | , |
Format: | Text |
Language: | English |
Published: |
MDPI
2023
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303718/ http://www.ncbi.nlm.nih.gov/pubmed/37367691 https://doi.org/10.3390/md21060366 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:10303718 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:10303718 2023-07-23T04:18:50+02:00 Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods Burke, Heather J. Kerton, Francesca 2023-06-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303718/ http://www.ncbi.nlm.nih.gov/pubmed/37367691 https://doi.org/10.3390/md21060366 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303718/ http://www.ncbi.nlm.nih.gov/pubmed/37367691 http://dx.doi.org/10.3390/md21060366 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Mar Drugs Article Text 2023 ftpubmed https://doi.org/10.3390/md21060366 2023-07-02T01:22:44Z Green extraction methods using a combination of mechanical, enzymatic, and green chemical treatments were evaluated for the sequential extraction of carotenoid pigments, protein, and chitin from crab processing discards. Key objectives included avoiding the use of hazardous chemical solvents, conducting as close to a 100% green extraction as possible, and developing simple processes to facilitate implementation into processing plants without the need for complicated and expensive equipment. Three crab bio-products were obtained: pigmented vegetable oil, pigmented protein powder, and chitin. Carotenoid extractions were performed using vegetable oils (corn, canola, and sunflower oils), giving between 24.85% and 37.93% astaxanthin recovery. Citric acid was used to demineralize the remaining material and afforded a pigmented protein powder. Three different proteases were used to deproteinate and isolate chitin in yields between 17.06% and 19.15%. The chitin was still highly colored and therefore decolorization was attempted using hydrogen peroxide. Characterization studies were conducted on each of the crab bio-products isolated including powder X-ray diffraction analysis on the chitin (80.18% crystallinity index, CI, achieved using green methods). Overall, three valuable bio-products could be obtained but further research is needed to obtain pigment-free chitin in an environmentally friendly manner. Text Chionoecetes opilio Snow crab PubMed Central (PMC) Marine Drugs 21 6 366 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Burke, Heather J. Kerton, Francesca Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods |
topic_facet |
Article |
description |
Green extraction methods using a combination of mechanical, enzymatic, and green chemical treatments were evaluated for the sequential extraction of carotenoid pigments, protein, and chitin from crab processing discards. Key objectives included avoiding the use of hazardous chemical solvents, conducting as close to a 100% green extraction as possible, and developing simple processes to facilitate implementation into processing plants without the need for complicated and expensive equipment. Three crab bio-products were obtained: pigmented vegetable oil, pigmented protein powder, and chitin. Carotenoid extractions were performed using vegetable oils (corn, canola, and sunflower oils), giving between 24.85% and 37.93% astaxanthin recovery. Citric acid was used to demineralize the remaining material and afforded a pigmented protein powder. Three different proteases were used to deproteinate and isolate chitin in yields between 17.06% and 19.15%. The chitin was still highly colored and therefore decolorization was attempted using hydrogen peroxide. Characterization studies were conducted on each of the crab bio-products isolated including powder X-ray diffraction analysis on the chitin (80.18% crystallinity index, CI, achieved using green methods). Overall, three valuable bio-products could be obtained but further research is needed to obtain pigment-free chitin in an environmentally friendly manner. |
format |
Text |
author |
Burke, Heather J. Kerton, Francesca |
author_facet |
Burke, Heather J. Kerton, Francesca |
author_sort |
Burke, Heather J. |
title |
Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods |
title_short |
Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods |
title_full |
Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods |
title_fullStr |
Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods |
title_full_unstemmed |
Sequential Extraction of Valuable Bio-Products from Snow Crab (Chionoecetes opilio) Processing Discards Using Eco-Friendly Methods |
title_sort |
sequential extraction of valuable bio-products from snow crab (chionoecetes opilio) processing discards using eco-friendly methods |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303718/ http://www.ncbi.nlm.nih.gov/pubmed/37367691 https://doi.org/10.3390/md21060366 |
genre |
Chionoecetes opilio Snow crab |
genre_facet |
Chionoecetes opilio Snow crab |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303718/ http://www.ncbi.nlm.nih.gov/pubmed/37367691 http://dx.doi.org/10.3390/md21060366 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_doi |
https://doi.org/10.3390/md21060366 |
container_title |
Marine Drugs |
container_volume |
21 |
container_issue |
6 |
container_start_page |
366 |
_version_ |
1772181482283991040 |