Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid
A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-l...
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ftpubmed:oai:pubmedcentral.nih.gov:10301472 2023-07-23T04:18:21+02:00 Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid Araujo, Pedro Iqbal, Sarah Arnø, Aleksander Espe, Marit Holen, Elisabeth 2023-06-13 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301472/ http://www.ncbi.nlm.nih.gov/pubmed/37375283 https://doi.org/10.3390/molecules28124728 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301472/ http://www.ncbi.nlm.nih.gov/pubmed/37375283 http://dx.doi.org/10.3390/molecules28124728 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Molecules Article Text 2023 ftpubmed https://doi.org/10.3390/molecules28124728 2023-07-02T01:16:01Z A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1–50 ng mL(−1)), limits of detection and quantification (0.05 and 0.1 ng mL(−1), respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses. Text Atlantic salmon PubMed Central (PMC) Molecules 28 12 4728 |
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Article Araujo, Pedro Iqbal, Sarah Arnø, Aleksander Espe, Marit Holen, Elisabeth Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
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A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1–50 ng mL(−1)), limits of detection and quantification (0.05 and 0.1 ng mL(−1), respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses. |
format |
Text |
author |
Araujo, Pedro Iqbal, Sarah Arnø, Aleksander Espe, Marit Holen, Elisabeth |
author_facet |
Araujo, Pedro Iqbal, Sarah Arnø, Aleksander Espe, Marit Holen, Elisabeth |
author_sort |
Araujo, Pedro |
title |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_short |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_full |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_fullStr |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_full_unstemmed |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_sort |
validation of a liquid–liquid extraction method to study the temporal production of d-series resolvins by head kidney cells from atlantic salmon (salmon salar) exposed to docosahexaenoic acid |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301472/ http://www.ncbi.nlm.nih.gov/pubmed/37375283 https://doi.org/10.3390/molecules28124728 |
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Atlantic salmon |
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Atlantic salmon |
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Molecules |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301472/ http://www.ncbi.nlm.nih.gov/pubmed/37375283 http://dx.doi.org/10.3390/molecules28124728 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_doi |
https://doi.org/10.3390/molecules28124728 |
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Molecules |
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