Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1.
One of the most concerning environmental problems is represented by petroleum and its derivatives causing contamination of aquatic and underground environments. In this work, the degradation treatment of diesel using Antarctic bacteria is proposed. Marinomonas sp. ef1 is a bacterial strain isolated...
Published in: | RSC Advances |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
The Royal Society of Chemistry
2023
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291279/ http://www.ncbi.nlm.nih.gov/pubmed/37377865 https://doi.org/10.1039/d3ra02536e |
id |
ftpubmed:oai:pubmedcentral.nih.gov:10291279 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:10291279 2023-07-23T04:15:48+02:00 Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. Zannotti, Marco Ramasamy, Kesava Priyan Loggi, Valentina Vassallo, Alberto Pucciarelli, Sandra Giovannetti, Rita 2023-06-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291279/ http://www.ncbi.nlm.nih.gov/pubmed/37377865 https://doi.org/10.1039/d3ra02536e en eng The Royal Society of Chemistry http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291279/ http://www.ncbi.nlm.nih.gov/pubmed/37377865 http://dx.doi.org/10.1039/d3ra02536e This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ RSC Adv Chemistry Text 2023 ftpubmed https://doi.org/10.1039/d3ra02536e 2023-07-02T00:45:54Z One of the most concerning environmental problems is represented by petroleum and its derivatives causing contamination of aquatic and underground environments. In this work, the degradation treatment of diesel using Antarctic bacteria is proposed. Marinomonas sp. ef1 is a bacterial strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. Its potential in the degradation of hydrocarbons commonly present in diesel oil were studied. The bacterial growth was evaluated in culturing conditions that resembled the marine environment with 1% (v/v) of either diesel or biodiesel added; in both cases, Marinomonas sp. ef1 was able to grow. The chemical oxygen demand measured after the incubation of bacteria with diesel decreased, demonstrating the ability of bacteria to use diesel hydrocarbons as a carbon source and degrade them. The metabolic potential of Marinomonas to degrade aromatic compounds was supported by the identification in the genome of sequences encoding various enzymes involved in benzene and naphthalene degradation. Moreover, in the presence of biodiesel, a fluorescent yellow pigment was produced; this was isolated, purified and characterized by UV-vis and fluorescence spectroscopy, leading to its identification as a pyoverdine. These results suggest that Marinomonas sp. ef1 can be used in hydrocarbon bioremediation and in the transformation of these pollutants in molecules of interest. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic RSC Advances 13 28 19276 19285 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Chemistry |
spellingShingle |
Chemistry Zannotti, Marco Ramasamy, Kesava Priyan Loggi, Valentina Vassallo, Alberto Pucciarelli, Sandra Giovannetti, Rita Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
topic_facet |
Chemistry |
description |
One of the most concerning environmental problems is represented by petroleum and its derivatives causing contamination of aquatic and underground environments. In this work, the degradation treatment of diesel using Antarctic bacteria is proposed. Marinomonas sp. ef1 is a bacterial strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. Its potential in the degradation of hydrocarbons commonly present in diesel oil were studied. The bacterial growth was evaluated in culturing conditions that resembled the marine environment with 1% (v/v) of either diesel or biodiesel added; in both cases, Marinomonas sp. ef1 was able to grow. The chemical oxygen demand measured after the incubation of bacteria with diesel decreased, demonstrating the ability of bacteria to use diesel hydrocarbons as a carbon source and degrade them. The metabolic potential of Marinomonas to degrade aromatic compounds was supported by the identification in the genome of sequences encoding various enzymes involved in benzene and naphthalene degradation. Moreover, in the presence of biodiesel, a fluorescent yellow pigment was produced; this was isolated, purified and characterized by UV-vis and fluorescence spectroscopy, leading to its identification as a pyoverdine. These results suggest that Marinomonas sp. ef1 can be used in hydrocarbon bioremediation and in the transformation of these pollutants in molecules of interest. |
format |
Text |
author |
Zannotti, Marco Ramasamy, Kesava Priyan Loggi, Valentina Vassallo, Alberto Pucciarelli, Sandra Giovannetti, Rita |
author_facet |
Zannotti, Marco Ramasamy, Kesava Priyan Loggi, Valentina Vassallo, Alberto Pucciarelli, Sandra Giovannetti, Rita |
author_sort |
Zannotti, Marco |
title |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_short |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_full |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_fullStr |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_full_unstemmed |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_sort |
hydrocarbon degradation strategy and pyoverdine production using the salt tolerant antarctic bacterium marinomonas sp. ef1. |
publisher |
The Royal Society of Chemistry |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291279/ http://www.ncbi.nlm.nih.gov/pubmed/37377865 https://doi.org/10.1039/d3ra02536e |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
RSC Adv |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291279/ http://www.ncbi.nlm.nih.gov/pubmed/37377865 http://dx.doi.org/10.1039/d3ra02536e |
op_rights |
This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
op_doi |
https://doi.org/10.1039/d3ra02536e |
container_title |
RSC Advances |
container_volume |
13 |
container_issue |
28 |
container_start_page |
19276 |
op_container_end_page |
19285 |
_version_ |
1772176830284955648 |