Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod
In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It allows an or...
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ftpubmed:oai:pubmedcentral.nih.gov:10290532 2023-07-23T04:21:58+02:00 Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H 2023-06-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10290532/ http://www.ncbi.nlm.nih.gov/pubmed/37361914 https://doi.org/10.1093/iob/obad020 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10290532/ http://www.ncbi.nlm.nih.gov/pubmed/37361914 http://dx.doi.org/10.1093/iob/obad020 © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Integrative and Comparative Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Integr Org Biol Article Text 2023 ftpubmed https://doi.org/10.1093/iob/obad020 2023-07-02T00:43:37Z In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It allows an organism to optimize the timing of reproduction by synchronizing offspring growth and development with periods of high food availability. In species that enter dormancy as pre-adults or adults, termination of diapause is marked by the resumption of physiological processes, an increase in metabolic rates and once transitioned into adulthood for females, the initiation of oogenesis. In many cases, individuals start feeding again and newly acquired resources become available to fuel egg production. However, in the subarctic capital-breeding copepod Neocalanus flemingeri, feeding is decoupled from oogenesis. Thus, optimizing reproduction limited by fixed resources such that all eggs are of high quality and fully-provisioned, requires regulation of the number of oocytes. However, it is unknown if and how this copepod limits oocyte formation. In this study, the phase in oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2′-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU-labeled cells peaking at 72 hours following diapause termination. Cell labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. The results suggest that oogenesis is sequential in N. flemingeri with formation of new oocytes starting within 24 hours of diapause termination and limited to the first few weeks. Lipid consumption during diapause was minimal and relatively modest initially. This early phase in the reproductive program precedes mid-oogenesis and vitellogenesis 2, when oocytes increase in ... Text Subarctic PubMed Central (PMC) Integrative Organismal Biology 5 1 |
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Article Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
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description |
In high-latitude environments where seasonal changes include periods of harsh conditions, many arthropods enter diapause, a period of dormancy that is hormonally regulated. Diapause is characterized by very low metabolism, resistance to environmental stress, and developmental arrest. It allows an organism to optimize the timing of reproduction by synchronizing offspring growth and development with periods of high food availability. In species that enter dormancy as pre-adults or adults, termination of diapause is marked by the resumption of physiological processes, an increase in metabolic rates and once transitioned into adulthood for females, the initiation of oogenesis. In many cases, individuals start feeding again and newly acquired resources become available to fuel egg production. However, in the subarctic capital-breeding copepod Neocalanus flemingeri, feeding is decoupled from oogenesis. Thus, optimizing reproduction limited by fixed resources such that all eggs are of high quality and fully-provisioned, requires regulation of the number of oocytes. However, it is unknown if and how this copepod limits oocyte formation. In this study, the phase in oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2′-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU-labeled cells peaking at 72 hours following diapause termination. Cell labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. The results suggest that oogenesis is sequential in N. flemingeri with formation of new oocytes starting within 24 hours of diapause termination and limited to the first few weeks. Lipid consumption during diapause was minimal and relatively modest initially. This early phase in the reproductive program precedes mid-oogenesis and vitellogenesis 2, when oocytes increase in ... |
format |
Text |
author |
Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H |
author_facet |
Monell, K J Roncalli, V Hopcroft, R R Hartline, D K Lenz, P H |
author_sort |
Monell, K J |
title |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_short |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_full |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_fullStr |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_full_unstemmed |
Post-Diapause DNA Replication during Oogenesis in a Capital-Breeding Copepod |
title_sort |
post-diapause dna replication during oogenesis in a capital-breeding copepod |
publisher |
Oxford University Press |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10290532/ http://www.ncbi.nlm.nih.gov/pubmed/37361914 https://doi.org/10.1093/iob/obad020 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Integr Org Biol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10290532/ http://www.ncbi.nlm.nih.gov/pubmed/37361914 http://dx.doi.org/10.1093/iob/obad020 |
op_rights |
© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Integrative and Comparative Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_doi |
https://doi.org/10.1093/iob/obad020 |
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Integrative Organismal Biology |
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5 |
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1772188372432846848 |