Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes
Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morphology, 18S...
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ftpubmed:oai:pubmedcentral.nih.gov:10246834 2023-07-02T03:33:14+02:00 Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes Suzuki, Hirono Détain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, René H Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J 2023-05-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246834/ http://www.ncbi.nlm.nih.gov/pubmed/37226528 https://doi.org/10.1093/femsec/fiad057 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246834/ http://www.ncbi.nlm.nih.gov/pubmed/37226528 http://dx.doi.org/10.1093/femsec/fiad057 © The Author(s) 2023. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. FEMS Microbiol Ecol Research Article Text 2023 ftpubmed https://doi.org/10.1093/femsec/fiad057 2023-06-11T01:07:36Z Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morphology, 18S rRNA gene and internal transcribed spacer 2 (ITS2) genetic markers. Phylogenetic and ITS2 rRNA secondary structure analyses assigned six isolates to the species Raphidonema nivale, Deuterostichococcus epilithicus, Chloromonas reticulata, and Xanthonema bristolianum. Two novel isolates belonging to the family Stichococcaceae (ARK-S05-19) and the genus Chloromonas (ARK-S08-19) were identified as potentially new species. In laboratory cultivation, differences in the growth rate and fatty acid profiles were observed between the strains. Chlorophyta were characterized by abundant C18:3n-3 fatty-acids with increases in C18:1n-9 in the stationary phase, whilst Xanthonema (Ochrophyta) was characterized by a large proportion of C20:5n-3, with increases in C16:1n-7 in the stationary phase. In a further experiment, lipid droplet formation was studied in C. reticulata at the single-cell level using imaging flow cytometry. Our study establishes new cultures of snow algae, reveals novel data on their biodiversity and biogeography, and provides an initial characterization of physiological traits that shape natural communities and their ecophysiological properties. Text Northern Norway PubMed Central (PMC) Norway FEMS Microbiology Ecology 99 6 |
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English |
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Research Article Suzuki, Hirono Détain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, René H Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
topic_facet |
Research Article |
description |
Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morphology, 18S rRNA gene and internal transcribed spacer 2 (ITS2) genetic markers. Phylogenetic and ITS2 rRNA secondary structure analyses assigned six isolates to the species Raphidonema nivale, Deuterostichococcus epilithicus, Chloromonas reticulata, and Xanthonema bristolianum. Two novel isolates belonging to the family Stichococcaceae (ARK-S05-19) and the genus Chloromonas (ARK-S08-19) were identified as potentially new species. In laboratory cultivation, differences in the growth rate and fatty acid profiles were observed between the strains. Chlorophyta were characterized by abundant C18:3n-3 fatty-acids with increases in C18:1n-9 in the stationary phase, whilst Xanthonema (Ochrophyta) was characterized by a large proportion of C20:5n-3, with increases in C16:1n-7 in the stationary phase. In a further experiment, lipid droplet formation was studied in C. reticulata at the single-cell level using imaging flow cytometry. Our study establishes new cultures of snow algae, reveals novel data on their biodiversity and biogeography, and provides an initial characterization of physiological traits that shape natural communities and their ecophysiological properties. |
format |
Text |
author |
Suzuki, Hirono Détain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, René H Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J |
author_facet |
Suzuki, Hirono Détain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, René H Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J |
author_sort |
Suzuki, Hirono |
title |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_short |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_full |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_fullStr |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_full_unstemmed |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_sort |
phylogeny and lipid profiles of snow-algae isolated from norwegian red-snow microbiomes |
publisher |
Oxford University Press |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246834/ http://www.ncbi.nlm.nih.gov/pubmed/37226528 https://doi.org/10.1093/femsec/fiad057 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
FEMS Microbiol Ecol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246834/ http://www.ncbi.nlm.nih.gov/pubmed/37226528 http://dx.doi.org/10.1093/femsec/fiad057 |
op_rights |
© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_doi |
https://doi.org/10.1093/femsec/fiad057 |
container_title |
FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
6 |
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1770273087224283136 |