A New Colorimetric Test for Accurate Determination of Plastic Biodegradation
As plastic waste is accumulating in both controlled waste management settings and natural settings, much research is devoted to search for solutions, also in the field of biodegradation. However, determining the biodegradability of plastics in natural environments remains a big challenge due to the...
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ftpubmed:oai:pubmedcentral.nih.gov:10221676 2023-06-18T03:38:09+02:00 A New Colorimetric Test for Accurate Determination of Plastic Biodegradation Mattelin, Valérie Verfaille, Lennert Kundu, Kankana De Wildeman, Stefaan Boon, Nico 2023-05-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221676/ http://www.ncbi.nlm.nih.gov/pubmed/37242886 https://doi.org/10.3390/polym15102311 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221676/ http://www.ncbi.nlm.nih.gov/pubmed/37242886 http://dx.doi.org/10.3390/polym15102311 © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Polymers (Basel) Article Text 2023 ftpubmed https://doi.org/10.3390/polym15102311 2023-06-04T01:01:47Z As plastic waste is accumulating in both controlled waste management settings and natural settings, much research is devoted to search for solutions, also in the field of biodegradation. However, determining the biodegradability of plastics in natural environments remains a big challenge due to the often very low biodegradation rates. Many standardised test methods for biodegradation in natural environments exist. These are often based on mineralisation rates in controlled conditions and are thus indirect measurements of biodegradation. It is of interest for both researchers and companies to have tests that are more rapid, easier, and more reliable to screen different ecosystems and/or niches for their plastic biodegradation potential. In this study, the goal is to validate a colorimetric test, based on carbon nanodots, to screen biodegradation of different types of plastics in natural environments. After introducing carbon nanodots into the matrix of the target plastic, a fluorescent signal is released upon plastic biodegradation. The in-house-made carbon nanodots were first confirmed regarding their biocompatibility and chemical and photostability. Subsequently, the effectivity of the developed method was evaluated positively by an enzymatic degradation test with polycaprolactone with Candida antarctica lipase B. Finally, validation experiments were performed with enriched microorganisms and real environmental samples (freshwater and seawater), of which the results were compared with parallel, frequently used biodegradation measures such as O(2) and CO(2), dissolved organic carbon, growth and pH, to assess the reliability of the test. Our results indicate that this colorimetric test is a good alternative to other methods, but a combination of different methods gives the most information. In conclusion, this colorimetric test is a good fit to screen, in high throughput, the depolymerisation of plastics in natural environments and under different conditions in the lab. Text Antarc* Antarctica PubMed Central (PMC) Polymers 15 10 2311 |
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Article Mattelin, Valérie Verfaille, Lennert Kundu, Kankana De Wildeman, Stefaan Boon, Nico A New Colorimetric Test for Accurate Determination of Plastic Biodegradation |
topic_facet |
Article |
description |
As plastic waste is accumulating in both controlled waste management settings and natural settings, much research is devoted to search for solutions, also in the field of biodegradation. However, determining the biodegradability of plastics in natural environments remains a big challenge due to the often very low biodegradation rates. Many standardised test methods for biodegradation in natural environments exist. These are often based on mineralisation rates in controlled conditions and are thus indirect measurements of biodegradation. It is of interest for both researchers and companies to have tests that are more rapid, easier, and more reliable to screen different ecosystems and/or niches for their plastic biodegradation potential. In this study, the goal is to validate a colorimetric test, based on carbon nanodots, to screen biodegradation of different types of plastics in natural environments. After introducing carbon nanodots into the matrix of the target plastic, a fluorescent signal is released upon plastic biodegradation. The in-house-made carbon nanodots were first confirmed regarding their biocompatibility and chemical and photostability. Subsequently, the effectivity of the developed method was evaluated positively by an enzymatic degradation test with polycaprolactone with Candida antarctica lipase B. Finally, validation experiments were performed with enriched microorganisms and real environmental samples (freshwater and seawater), of which the results were compared with parallel, frequently used biodegradation measures such as O(2) and CO(2), dissolved organic carbon, growth and pH, to assess the reliability of the test. Our results indicate that this colorimetric test is a good alternative to other methods, but a combination of different methods gives the most information. In conclusion, this colorimetric test is a good fit to screen, in high throughput, the depolymerisation of plastics in natural environments and under different conditions in the lab. |
format |
Text |
author |
Mattelin, Valérie Verfaille, Lennert Kundu, Kankana De Wildeman, Stefaan Boon, Nico |
author_facet |
Mattelin, Valérie Verfaille, Lennert Kundu, Kankana De Wildeman, Stefaan Boon, Nico |
author_sort |
Mattelin, Valérie |
title |
A New Colorimetric Test for Accurate Determination of Plastic Biodegradation |
title_short |
A New Colorimetric Test for Accurate Determination of Plastic Biodegradation |
title_full |
A New Colorimetric Test for Accurate Determination of Plastic Biodegradation |
title_fullStr |
A New Colorimetric Test for Accurate Determination of Plastic Biodegradation |
title_full_unstemmed |
A New Colorimetric Test for Accurate Determination of Plastic Biodegradation |
title_sort |
new colorimetric test for accurate determination of plastic biodegradation |
publisher |
MDPI |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221676/ http://www.ncbi.nlm.nih.gov/pubmed/37242886 https://doi.org/10.3390/polym15102311 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Polymers (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221676/ http://www.ncbi.nlm.nih.gov/pubmed/37242886 http://dx.doi.org/10.3390/polym15102311 |
op_rights |
© 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_doi |
https://doi.org/10.3390/polym15102311 |
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Polymers |
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15 |
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10 |
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2311 |
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