Application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a New Zealand abalone
Natural history collections worldwide contain a plethora of mollusc shells. Recent studies have detailed the sequencing of DNA extracted from shells up to thousands of years old and from various taphonomic and preservational contexts. However, previous approaches have largely addressed methodologica...
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ftpubmed:oai:pubmedcentral.nih.gov:10087340 2023-06-06T11:48:05+02:00 Application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a New Zealand abalone Walton, Kerry Scarsbrook, Lachie Mitchell, Kieren J. Verry, Alexander J. F. Marshall, Bruce A. Rawlence, Nicolas J. Spencer, Hamish G. 2022-08-21 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087340/ http://www.ncbi.nlm.nih.gov/pubmed/35951485 https://doi.org/10.1111/1755-0998.13696 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087340/ http://www.ncbi.nlm.nih.gov/pubmed/35951485 http://dx.doi.org/10.1111/1755-0998.13696 © 2022 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Mol Ecol Resour RESOURCE ARTICLES Text 2022 ftpubmed https://doi.org/10.1111/1755-0998.13696 2023-04-16T00:56:29Z Natural history collections worldwide contain a plethora of mollusc shells. Recent studies have detailed the sequencing of DNA extracted from shells up to thousands of years old and from various taphonomic and preservational contexts. However, previous approaches have largely addressed methodological rather than evolutionary research questions. Here, we report the generation of DNA sequence data from mollusc shells using such techniques, applied to Haliotis virginea Gmelin, 1791, a New Zealand abalone, in which morphological variation has led to the recognition of several forms and subspecies. We successfully recovered near‐complete mitogenomes from 22 specimens including 12 dry‐preserved shells up to 60 years old. We used a combination of palaeogenetic techniques that have not previously been applied to shell, including DNA extraction optimized for ultra‐short fragments and hybridization‐capture of single‐stranded DNA libraries. Phylogenetic analyses revealed three major, well‐supported clades comprising samples from: (1) The Three Kings Islands; (2) the Auckland, Chatham and Antipodes Islands; and (3) mainland New Zealand and Campbell Island. This phylogeographic structure does not correspond to the currently recognized forms. Critically, our nonreliance on freshly collected or ethanol‐preserved samples enabled inclusion of topotypes of all recognized subspecies as well as additional difficult‐to‐sample populations. Broader application of these comparatively cost‐effective and reliable methods to modern, historical, archaeological and palaeontological shell samples has the potential to revolutionize invertebrate genetic research. Text Antipodes Islands PubMed Central (PMC) Campbell Island ENVELOPE(169.500,169.500,-52.500,-52.500) New Zealand Molecular Ecology Resources 23 1 118 130 |
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RESOURCE ARTICLES Walton, Kerry Scarsbrook, Lachie Mitchell, Kieren J. Verry, Alexander J. F. Marshall, Bruce A. Rawlence, Nicolas J. Spencer, Hamish G. Application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a New Zealand abalone |
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RESOURCE ARTICLES |
description |
Natural history collections worldwide contain a plethora of mollusc shells. Recent studies have detailed the sequencing of DNA extracted from shells up to thousands of years old and from various taphonomic and preservational contexts. However, previous approaches have largely addressed methodological rather than evolutionary research questions. Here, we report the generation of DNA sequence data from mollusc shells using such techniques, applied to Haliotis virginea Gmelin, 1791, a New Zealand abalone, in which morphological variation has led to the recognition of several forms and subspecies. We successfully recovered near‐complete mitogenomes from 22 specimens including 12 dry‐preserved shells up to 60 years old. We used a combination of palaeogenetic techniques that have not previously been applied to shell, including DNA extraction optimized for ultra‐short fragments and hybridization‐capture of single‐stranded DNA libraries. Phylogenetic analyses revealed three major, well‐supported clades comprising samples from: (1) The Three Kings Islands; (2) the Auckland, Chatham and Antipodes Islands; and (3) mainland New Zealand and Campbell Island. This phylogeographic structure does not correspond to the currently recognized forms. Critically, our nonreliance on freshly collected or ethanol‐preserved samples enabled inclusion of topotypes of all recognized subspecies as well as additional difficult‐to‐sample populations. Broader application of these comparatively cost‐effective and reliable methods to modern, historical, archaeological and palaeontological shell samples has the potential to revolutionize invertebrate genetic research. |
format |
Text |
author |
Walton, Kerry Scarsbrook, Lachie Mitchell, Kieren J. Verry, Alexander J. F. Marshall, Bruce A. Rawlence, Nicolas J. Spencer, Hamish G. |
author_facet |
Walton, Kerry Scarsbrook, Lachie Mitchell, Kieren J. Verry, Alexander J. F. Marshall, Bruce A. Rawlence, Nicolas J. Spencer, Hamish G. |
author_sort |
Walton, Kerry |
title |
Application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a New Zealand abalone |
title_short |
Application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a New Zealand abalone |
title_full |
Application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a New Zealand abalone |
title_fullStr |
Application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a New Zealand abalone |
title_full_unstemmed |
Application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a New Zealand abalone |
title_sort |
application of palaeogenetic techniques to historic mollusc shells reveals phylogeographic structure in a new zealand abalone |
publisher |
John Wiley and Sons Inc. |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087340/ http://www.ncbi.nlm.nih.gov/pubmed/35951485 https://doi.org/10.1111/1755-0998.13696 |
long_lat |
ENVELOPE(169.500,169.500,-52.500,-52.500) |
geographic |
Campbell Island New Zealand |
geographic_facet |
Campbell Island New Zealand |
genre |
Antipodes Islands |
genre_facet |
Antipodes Islands |
op_source |
Mol Ecol Resour |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087340/ http://www.ncbi.nlm.nih.gov/pubmed/35951485 http://dx.doi.org/10.1111/1755-0998.13696 |
op_rights |
© 2022 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_doi |
https://doi.org/10.1111/1755-0998.13696 |
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Molecular Ecology Resources |
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23 |
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1 |
container_start_page |
118 |
op_container_end_page |
130 |
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1767953980754755584 |