Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater
BACKGROUND: Yeasts are gaining attention as alternative ingredients in aquafeeds. However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited. Thus, the present study investigates the effects of yeast and processing on composition, div...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074822/ http://www.ncbi.nlm.nih.gov/pubmed/37016467 https://doi.org/10.1186/s42523-023-00242-y |
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ftpubmed:oai:pubmedcentral.nih.gov:10074822 2023-05-15T15:32:13+02:00 Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater Agboola, Jeleel O. Rocha, Sérgio D. C. Mensah, Dominic D. Hansen, Jon Ø. Øyås, Ove Lapeña, David Mydland, Liv T. Arntzen, Magnus Ø. Horn, Svein J. Øverland, Margareth 2023-04-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074822/ http://www.ncbi.nlm.nih.gov/pubmed/37016467 https://doi.org/10.1186/s42523-023-00242-y en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074822/ http://www.ncbi.nlm.nih.gov/pubmed/37016467 http://dx.doi.org/10.1186/s42523-023-00242-y © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . Anim Microbiome Research Text 2023 ftpubmed https://doi.org/10.1186/s42523-023-00242-y 2023-04-09T01:02:29Z BACKGROUND: Yeasts are gaining attention as alternative ingredients in aquafeeds. However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited. Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet. Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA). In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA). Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models. RESULTS: The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet. The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets). Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish. The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets. The gut microbiota of fish fed AWA diet was highly dominated by the family Bacillaceae. CONCLUSIONS: The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish. The inclusion of inactivated yeasts did not alter the modulation ... Text Atlantic salmon Salmo salar PubMed Central (PMC) Animal Microbiome 5 1 |
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Research Agboola, Jeleel O. Rocha, Sérgio D. C. Mensah, Dominic D. Hansen, Jon Ø. Øyås, Ove Lapeña, David Mydland, Liv T. Arntzen, Magnus Ø. Horn, Svein J. Øverland, Margareth Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater |
topic_facet |
Research |
description |
BACKGROUND: Yeasts are gaining attention as alternative ingredients in aquafeeds. However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited. Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet. Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA). In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA). Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models. RESULTS: The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet. The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets). Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish. The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets. The gut microbiota of fish fed AWA diet was highly dominated by the family Bacillaceae. CONCLUSIONS: The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish. The inclusion of inactivated yeasts did not alter the modulation ... |
format |
Text |
author |
Agboola, Jeleel O. Rocha, Sérgio D. C. Mensah, Dominic D. Hansen, Jon Ø. Øyås, Ove Lapeña, David Mydland, Liv T. Arntzen, Magnus Ø. Horn, Svein J. Øverland, Margareth |
author_facet |
Agboola, Jeleel O. Rocha, Sérgio D. C. Mensah, Dominic D. Hansen, Jon Ø. Øyås, Ove Lapeña, David Mydland, Liv T. Arntzen, Magnus Ø. Horn, Svein J. Øverland, Margareth |
author_sort |
Agboola, Jeleel O. |
title |
Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater |
title_short |
Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater |
title_full |
Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater |
title_fullStr |
Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater |
title_full_unstemmed |
Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater |
title_sort |
effect of yeast species and processing on intestinal microbiota of atlantic salmon (salmo salar) fed soybean meal-based diets in seawater |
publisher |
BioMed Central |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074822/ http://www.ncbi.nlm.nih.gov/pubmed/37016467 https://doi.org/10.1186/s42523-023-00242-y |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Anim Microbiome |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074822/ http://www.ncbi.nlm.nih.gov/pubmed/37016467 http://dx.doi.org/10.1186/s42523-023-00242-y |
op_rights |
© The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_doi |
https://doi.org/10.1186/s42523-023-00242-y |
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Animal Microbiome |
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