On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum
Polygonum viviparum L. (PV) is a widely used resource plant with high medicinal, feeding and ecological values. Our studies show that PV has strong antioxidant activity. However, up to date, the antioxidant activity and components in other parts were not fully elucidated. In the present study, a new...
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ftpubmed:oai:pubmedcentral.nih.gov:10035567 2023-05-15T18:03:01+02:00 On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum Qian, Zheng-ming Cheng, Xin-jie Wang, Qiao Huang, Qi Jin, Li-ling Ma, Ya-fei Xie, Jia-sheng Li, De-qiang 2023-03-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035567/ https://doi.org/10.1039/d2ra08247k en eng The Royal Society of Chemistry http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035567/ http://dx.doi.org/10.1039/d2ra08247k This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ RSC Adv Chemistry Text 2023 ftpubmed https://doi.org/10.1039/d2ra08247k 2023-03-26T02:20:28Z Polygonum viviparum L. (PV) is a widely used resource plant with high medicinal, feeding and ecological values. Our studies show that PV has strong antioxidant activity. However, up to date, the antioxidant activity and components in other parts were not fully elucidated. In the present study, a new online pre-column ferric ion reducing antioxidant power (FRAP)-based antioxidant reaction coupled with high performance liquid chromatography-diode array detector-quadrupole-time-of-flight mass spectrometry (HPLC-DAD-TOF/MS) was developed for rapid and high-throughput screening of natural antioxidants from three different parts of PV including stems and leaves, fruits and rhizomes. In this procedure, it was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be greatly diminished or vanish after incubating with the FRAP. The online incubation conditions including mixed ratios of sample and FRAP solution and reaction times were firstly optimized with six standards. Then, the repeatability of the screening system was evaluated by analysis of the samples of stems and leaves of PV. As a result, a total of 21 compounds mainly including flavonoids and phenolic acids were screened from the three parts of PV. In conclusion, the present study provided a simple and effective strategy to rapidly screen antioxidants in natural products. Text Polygonum viviparum PubMed Central (PMC) RSC Advances 13 14 9585 9594 |
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Chemistry Qian, Zheng-ming Cheng, Xin-jie Wang, Qiao Huang, Qi Jin, Li-ling Ma, Ya-fei Xie, Jia-sheng Li, De-qiang On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum |
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Chemistry |
description |
Polygonum viviparum L. (PV) is a widely used resource plant with high medicinal, feeding and ecological values. Our studies show that PV has strong antioxidant activity. However, up to date, the antioxidant activity and components in other parts were not fully elucidated. In the present study, a new online pre-column ferric ion reducing antioxidant power (FRAP)-based antioxidant reaction coupled with high performance liquid chromatography-diode array detector-quadrupole-time-of-flight mass spectrometry (HPLC-DAD-TOF/MS) was developed for rapid and high-throughput screening of natural antioxidants from three different parts of PV including stems and leaves, fruits and rhizomes. In this procedure, it was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be greatly diminished or vanish after incubating with the FRAP. The online incubation conditions including mixed ratios of sample and FRAP solution and reaction times were firstly optimized with six standards. Then, the repeatability of the screening system was evaluated by analysis of the samples of stems and leaves of PV. As a result, a total of 21 compounds mainly including flavonoids and phenolic acids were screened from the three parts of PV. In conclusion, the present study provided a simple and effective strategy to rapidly screen antioxidants in natural products. |
format |
Text |
author |
Qian, Zheng-ming Cheng, Xin-jie Wang, Qiao Huang, Qi Jin, Li-ling Ma, Ya-fei Xie, Jia-sheng Li, De-qiang |
author_facet |
Qian, Zheng-ming Cheng, Xin-jie Wang, Qiao Huang, Qi Jin, Li-ling Ma, Ya-fei Xie, Jia-sheng Li, De-qiang |
author_sort |
Qian, Zheng-ming |
title |
On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum |
title_short |
On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum |
title_full |
On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum |
title_fullStr |
On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum |
title_full_unstemmed |
On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum |
title_sort |
on-line pre-column frap-based antioxidant reaction coupled with hplc-dad-tof/ms for rapid screening of natural antioxidants from different parts of polygonum viviparum |
publisher |
The Royal Society of Chemistry |
publishDate |
2023 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035567/ https://doi.org/10.1039/d2ra08247k |
genre |
Polygonum viviparum |
genre_facet |
Polygonum viviparum |
op_source |
RSC Adv |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035567/ http://dx.doi.org/10.1039/d2ra08247k |
op_rights |
This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
op_doi |
https://doi.org/10.1039/d2ra08247k |
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