Fast and cost-efficient species identification of Atlantic salmon (Salmo salar L.), brown trout (Salmo trutta), and their hybrids using a single SNP marker.

A workflow for developing a cost- and time-efficient, single nucleotide polymorphism (SNP)-based assay for species and hybrid identification is described. In a reference set (n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon (Salmo salar L., n =...

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Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Aykanat, Tutku, Balatsou, Athina, Kähkönen, Kirsi, Syrjänen, Jukka T, Janhunen, Matti, Leinonen, Tuomas, Prokkola, Jenni M, Norrgård, Johnny R, Piccolo, John J
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2025
Subjects:
KASP SNP‐assays
conservation genetics
diagnostic locus
hybridization
species identification
Atlantic salmon
Salmo salar
Online Access:https://doi.org/10.1111/jfb.16032
https://pubmed.ncbi.nlm.nih.gov/39689975
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038774/
Description
Summary:A workflow for developing a cost- and time-efficient, single nucleotide polymorphism (SNP)-based assay for species and hybrid identification is described. In a reference set (n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon (Salmo salar L., n = 23) and brown trout (Salmo trutta, n = 23), with 100% accuracy. Furthermore, species and hybrid identification using field-collected embryos had 98.1% concordance (155/158) to more expensive and time-consuming methods that utilized multiple SNP markers. The method can be integrated into management and conservation plans to quantify species' spawning distribution and hybridization rates.

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