Genomic Sequence of
Frequently, diseases in aquaculture have been fought indiscriminately with the use of antibiotics, which has led to the development and dissemination of (multiple) antibiotic resistances in bacteria. Consequently, it is necessary to look for alternative and complementary approaches to chemotheraphy...
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Online Access: | https://doi.org/10.3390/vetsci11080365 https://pubmed.ncbi.nlm.nih.gov/39195819 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359882/ |
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ftpubmed:39195819 2024-09-15T18:25:26+00:00 Genomic Sequence of Díaz-Formoso, Lara Contente, Diogo Feito, Javier Hernández, Pablo E Borrero, Juan Muñoz-Atienza, Estefanía Cintas, Luis M 2024 Aug 10 https://doi.org/10.3390/vetsci11080365 https://pubmed.ncbi.nlm.nih.gov/39195819 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359882/ eng eng MDPI https://doi.org/10.3390/vetsci11080365 https://pubmed.ncbi.nlm.nih.gov/39195819 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359882/ Vet Sci ISSN:2306-7381 Volume:11 Issue:8 antimicrobial peptides lactic acid bacteria (LAB) marine fish probiotics whole-genome sequencing (WGS) Journal Article 2024 ftpubmed https://doi.org/10.3390/vetsci11080365 2024-08-31T16:02:00Z Frequently, diseases in aquaculture have been fought indiscriminately with the use of antibiotics, which has led to the development and dissemination of (multiple) antibiotic resistances in bacteria. Consequently, it is necessary to look for alternative and complementary approaches to chemotheraphy that are safe for humans, animals, and the environment, such as the use of probiotics in fish farming. The objective of this work was the Whole-Genome Sequencing (WGS) and bioinformatic and functional analyses of S. salivarius MDI13 and L. sakei MEI5, two LAB strains isolated from the gut of commercial European hakes (M. merluccius, L.) caught in the Northeast Atlantic Ocean. The WGS and bioinformatic and functional analyses confirmed the lack of transferable antibiotic resistance genes, the lack of virulence and pathogenicity issues, and their potentially probiotic characteristics. Specifically, genes involved in adhesion and aggregation, vitamin biosynthesis, and amino acid metabolism were detected in both strains. In addition, genes related to lactic acid production, active metabolism, and/or adaptation to stress and adverse conditions in the host gastrointestinal tract were detected in L. sakei MEI5. Moreover, a gene cluster encoding three bacteriocins (SlvV, BlpK, and BlpE) was identified in the genome of S. salivarius MDI13. The in vitro-synthesized bacteriocin BlpK showed antimicrobial activity against the ichthyopathogens Lc. garvieae and S. parauberis. Altogether, our results suggest that S. salivarius MDI13 and L. sakei MEI5 have a strong potential as probiotics to prevent fish diseases in aquaculture as an appropriate alternative/complementary strategy to the use of antibiotics. Article in Journal/Newspaper Northeast Atlantic PubMed Central (PMC) Veterinary Sciences 11 8 365 |
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English |
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antimicrobial peptides lactic acid bacteria (LAB) marine fish probiotics whole-genome sequencing (WGS) |
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antimicrobial peptides lactic acid bacteria (LAB) marine fish probiotics whole-genome sequencing (WGS) Díaz-Formoso, Lara Contente, Diogo Feito, Javier Hernández, Pablo E Borrero, Juan Muñoz-Atienza, Estefanía Cintas, Luis M Genomic Sequence of |
topic_facet |
antimicrobial peptides lactic acid bacteria (LAB) marine fish probiotics whole-genome sequencing (WGS) |
description |
Frequently, diseases in aquaculture have been fought indiscriminately with the use of antibiotics, which has led to the development and dissemination of (multiple) antibiotic resistances in bacteria. Consequently, it is necessary to look for alternative and complementary approaches to chemotheraphy that are safe for humans, animals, and the environment, such as the use of probiotics in fish farming. The objective of this work was the Whole-Genome Sequencing (WGS) and bioinformatic and functional analyses of S. salivarius MDI13 and L. sakei MEI5, two LAB strains isolated from the gut of commercial European hakes (M. merluccius, L.) caught in the Northeast Atlantic Ocean. The WGS and bioinformatic and functional analyses confirmed the lack of transferable antibiotic resistance genes, the lack of virulence and pathogenicity issues, and their potentially probiotic characteristics. Specifically, genes involved in adhesion and aggregation, vitamin biosynthesis, and amino acid metabolism were detected in both strains. In addition, genes related to lactic acid production, active metabolism, and/or adaptation to stress and adverse conditions in the host gastrointestinal tract were detected in L. sakei MEI5. Moreover, a gene cluster encoding three bacteriocins (SlvV, BlpK, and BlpE) was identified in the genome of S. salivarius MDI13. The in vitro-synthesized bacteriocin BlpK showed antimicrobial activity against the ichthyopathogens Lc. garvieae and S. parauberis. Altogether, our results suggest that S. salivarius MDI13 and L. sakei MEI5 have a strong potential as probiotics to prevent fish diseases in aquaculture as an appropriate alternative/complementary strategy to the use of antibiotics. |
format |
Article in Journal/Newspaper |
author |
Díaz-Formoso, Lara Contente, Diogo Feito, Javier Hernández, Pablo E Borrero, Juan Muñoz-Atienza, Estefanía Cintas, Luis M |
author_facet |
Díaz-Formoso, Lara Contente, Diogo Feito, Javier Hernández, Pablo E Borrero, Juan Muñoz-Atienza, Estefanía Cintas, Luis M |
author_sort |
Díaz-Formoso, Lara |
title |
Genomic Sequence of |
title_short |
Genomic Sequence of |
title_full |
Genomic Sequence of |
title_fullStr |
Genomic Sequence of |
title_full_unstemmed |
Genomic Sequence of |
title_sort |
genomic sequence of |
publisher |
MDPI |
publishDate |
2024 |
url |
https://doi.org/10.3390/vetsci11080365 https://pubmed.ncbi.nlm.nih.gov/39195819 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359882/ |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Vet Sci ISSN:2306-7381 Volume:11 Issue:8 |
op_relation |
https://doi.org/10.3390/vetsci11080365 https://pubmed.ncbi.nlm.nih.gov/39195819 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359882/ |
op_doi |
https://doi.org/10.3390/vetsci11080365 |
container_title |
Veterinary Sciences |
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11 |
container_issue |
8 |
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365 |
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1810465953819918336 |