Long-term hypoxia-induced physiological response in turbot Scophthalmus maximus L.
Hypoxia affects fish's survival, growth, and physiological metabolism processes. In this study, turbot plasma glucose and cortisol contents, hepatic glycolysis (hexokinase [HK], phosphofructokinase [PFK], pyruvate kinase [PK]) and lipolysis (fatty acid synthetase [FAS], lipoprotein lipase [LPL]...
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ftpubmed:39190213 2024-09-15T18:34:00+00:00 Long-term hypoxia-induced physiological response in turbot Scophthalmus maximus L. Jia, Yudong Wang, Feng Chen, Shuaiyu Wang, Jiawei Gao, Yuntao 2024 Aug 27 https://doi.org/10.1007/s10695-024-01398-3 https://pubmed.ncbi.nlm.nih.gov/39190213 eng eng Springer https://doi.org/10.1007/s10695-024-01398-3 https://pubmed.ncbi.nlm.nih.gov/39190213 © 2024. The Author(s), under exclusive licence to Springer Nature B.V. Fish Physiol Biochem ISSN:1573-5168 Gill histology Hypoxia stress Metabolism Oxidative stress Turbot Scophthalmus maximus. L Journal Article 2024 ftpubmed https://doi.org/10.1007/s10695-024-01398-3 2024-08-27T16:03:00Z Hypoxia affects fish's survival, growth, and physiological metabolism processes. In this study, turbot plasma glucose and cortisol contents, hepatic glycolysis (hexokinase [HK], phosphofructokinase [PFK], pyruvate kinase [PK]) and lipolysis (fatty acid synthetase [FAS], lipoprotein lipase [LPL]) enzyme activities, anti-oxidant enzyme (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GSH-Px]) activities, malondialdehyde (MDA), lactate and glycogen contents, gill histological parameters (lamellar length [SLL], width [SLW], interlamellar distance [ID]), respiratory frequency (RF), the proportion of the secondary lamellae available for gas exchange (PAGE), and hifs (hif-1α, hif-2α, hif-3α) expression were determined during long-term hypoxia and reoxygenation. Results showed that long-term hypoxia (3.34 ± 0.17 mg L-1) significantly elevated plasma cortisol and glucose contents; increased hepatic HK, PK, PFK, FAS, and LPL activity; decreased hepatic glycogen, lactate contents, and lipid drop numbers; and caused changes of hepatocyte (vacuolation, pyknotic, and lytic nucleus) after treatment for 4 weeks. Hepatic SOD, CAT, GSH-Px activity, and MDA contents; lamellar perimeter, SLL, ID, RF, and PAGE; and hepatic hif-1α, hif-2α, and hif-3α manifested similar results. Meanwhile, hif-1α is significantly higher than hif-2α, and hif-3α. Interestingly, females and males demonstrated no sex dimorphism significantly different from the above parameters (except hepatic FAS, LPL activity, and lipid drop number) under hypoxia. The above parameters recovered to normal levels after reoxygenation treatment for 4 weeks. Thus, long-term hypoxia promotes turbot hepatic glycogenolysis and lipolysis, induces oxidative damage and stimulates hepatic antioxidant capacity, and alters gill morphology to satisfy insufficient energy demand and alleviate potential damage, while hif-1α plays critical roles in the above physiological process. Article in Journal/Newspaper Scophthalmus maximus Turbot PubMed Central (PMC) Fish Physiology and Biochemistry |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Gill histology Hypoxia stress Metabolism Oxidative stress Turbot Scophthalmus maximus. L |
spellingShingle |
Gill histology Hypoxia stress Metabolism Oxidative stress Turbot Scophthalmus maximus. L Jia, Yudong Wang, Feng Chen, Shuaiyu Wang, Jiawei Gao, Yuntao Long-term hypoxia-induced physiological response in turbot Scophthalmus maximus L. |
topic_facet |
Gill histology Hypoxia stress Metabolism Oxidative stress Turbot Scophthalmus maximus. L |
description |
Hypoxia affects fish's survival, growth, and physiological metabolism processes. In this study, turbot plasma glucose and cortisol contents, hepatic glycolysis (hexokinase [HK], phosphofructokinase [PFK], pyruvate kinase [PK]) and lipolysis (fatty acid synthetase [FAS], lipoprotein lipase [LPL]) enzyme activities, anti-oxidant enzyme (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GSH-Px]) activities, malondialdehyde (MDA), lactate and glycogen contents, gill histological parameters (lamellar length [SLL], width [SLW], interlamellar distance [ID]), respiratory frequency (RF), the proportion of the secondary lamellae available for gas exchange (PAGE), and hifs (hif-1α, hif-2α, hif-3α) expression were determined during long-term hypoxia and reoxygenation. Results showed that long-term hypoxia (3.34 ± 0.17 mg L-1) significantly elevated plasma cortisol and glucose contents; increased hepatic HK, PK, PFK, FAS, and LPL activity; decreased hepatic glycogen, lactate contents, and lipid drop numbers; and caused changes of hepatocyte (vacuolation, pyknotic, and lytic nucleus) after treatment for 4 weeks. Hepatic SOD, CAT, GSH-Px activity, and MDA contents; lamellar perimeter, SLL, ID, RF, and PAGE; and hepatic hif-1α, hif-2α, and hif-3α manifested similar results. Meanwhile, hif-1α is significantly higher than hif-2α, and hif-3α. Interestingly, females and males demonstrated no sex dimorphism significantly different from the above parameters (except hepatic FAS, LPL activity, and lipid drop number) under hypoxia. The above parameters recovered to normal levels after reoxygenation treatment for 4 weeks. Thus, long-term hypoxia promotes turbot hepatic glycogenolysis and lipolysis, induces oxidative damage and stimulates hepatic antioxidant capacity, and alters gill morphology to satisfy insufficient energy demand and alleviate potential damage, while hif-1α plays critical roles in the above physiological process. |
format |
Article in Journal/Newspaper |
author |
Jia, Yudong Wang, Feng Chen, Shuaiyu Wang, Jiawei Gao, Yuntao |
author_facet |
Jia, Yudong Wang, Feng Chen, Shuaiyu Wang, Jiawei Gao, Yuntao |
author_sort |
Jia, Yudong |
title |
Long-term hypoxia-induced physiological response in turbot Scophthalmus maximus L. |
title_short |
Long-term hypoxia-induced physiological response in turbot Scophthalmus maximus L. |
title_full |
Long-term hypoxia-induced physiological response in turbot Scophthalmus maximus L. |
title_fullStr |
Long-term hypoxia-induced physiological response in turbot Scophthalmus maximus L. |
title_full_unstemmed |
Long-term hypoxia-induced physiological response in turbot Scophthalmus maximus L. |
title_sort |
long-term hypoxia-induced physiological response in turbot scophthalmus maximus l. |
publisher |
Springer |
publishDate |
2024 |
url |
https://doi.org/10.1007/s10695-024-01398-3 https://pubmed.ncbi.nlm.nih.gov/39190213 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Fish Physiol Biochem ISSN:1573-5168 |
op_relation |
https://doi.org/10.1007/s10695-024-01398-3 https://pubmed.ncbi.nlm.nih.gov/39190213 |
op_rights |
© 2024. The Author(s), under exclusive licence to Springer Nature B.V. |
op_doi |
https://doi.org/10.1007/s10695-024-01398-3 |
container_title |
Fish Physiology and Biochemistry |
_version_ |
1810475730855788544 |