Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil.
A total of 231 blood samples from wild mammals belonging to the orders Rodentia (n = 142) and Didelphimorphia (n = 89) were screened by real-time PCR assay (qPCR), being six Rhipidomys sp., 118 Thrichomys laurentius, nine Rattus rattus, four Kerodon rupestris, five Necromys lasiurus, 42 Didelphis al...
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ftpubmed:39127138 2024-09-30T14:41:44+00:00 Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil. Torres-Santos, Paula Talita Evaristo, Anna Maria da Cruz Ferreira Santos, Josenilton Rodrigues Collere, Flávia Carolina Meira Vieira, Thállitha Samih Wischral Jayme Pereira, Luiz Cezar Machado Nicola, Patricia Avello Vieira, Rafael Felipe da Costa Horta, Mauricio Claudio 2024 Oct https://doi.org/10.1016/j.actatropica.2024.107334 https://pubmed.ncbi.nlm.nih.gov/39127138 eng eng Elsevier Science https://doi.org/10.1016/j.actatropica.2024.107334 https://pubmed.ncbi.nlm.nih.gov/39127138 Copyright © 2024. Published by Elsevier B.V. Acta Trop ISSN:1873-6254 Volume:258 16S rRNA 23S rRNA Hemotropic mycoplasmas Sequencing qPCR Journal Article 2024 ftpubmed https://doi.org/10.1016/j.actatropica.2024.107334 2024-09-01T16:02:00Z A total of 231 blood samples from wild mammals belonging to the orders Rodentia (n = 142) and Didelphimorphia (n = 89) were screened by real-time PCR assay (qPCR), being six Rhipidomys sp., 118 Thrichomys laurentius, nine Rattus rattus, four Kerodon rupestris, five Necromys lasiurus, 42 Didelphis albiventris and 47 Monodelphis domestica. Results using qPCR showed that 32 of the total 231 (13.85 %) samples were positive for hemoplasma sequences of the 16S rRNA gene. Sequences from two D. albiventris showed 99.77-99.89 % identity with 'Candidatus Mycoplasma haemoalbiventris' and 99.09 % with 'Candidatus Mycoplasma haemodidelphidis', respectively. Furthermore, one M. domestica and five T. laurentius showed 99.72-99.77 % identity with Mycoplasma sp., and one K. rupestris showed 98.13 % identity with 'Candidatus Mycoplasma haematohydrochaerus'; and from two Rattus rattus showed 99.65-99.89 % identity with Mycoplasma sp. and 'Candidatus Mycoplasma haemomuris'. The 23S rRNA gene sequences obtained from the two D. albiventris showed 100 % identity with 'Ca. M. haemoalbiventris' whereas the sequences from the R. rattus showed only 85.31 % identity with 'Candidatus Mycoplasma haematohydrochaerus'. Two T. laurentius and one K. rupestris showed 84.66-92.97 % identity with 'Candidatus Mycoplasma haemosphiggurus'. Based on phylogenetic and Neighbor-Net network analyses of the 16S and 23S rRNA genes, potential novel species are described. In addition, 'Ca. M. haemoalbiventris' was detected in Didelphis albiventris, and Mycoplasma sp. was detected in Rattus sp. rodents from the Caatinga biome, Brazil. Article in Journal/Newspaper Rattus rattus PubMed Central (PMC) Acta Tropica 258 107334 |
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Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
16S rRNA 23S rRNA Hemotropic mycoplasmas Sequencing qPCR |
spellingShingle |
16S rRNA 23S rRNA Hemotropic mycoplasmas Sequencing qPCR Torres-Santos, Paula Talita Evaristo, Anna Maria da Cruz Ferreira Santos, Josenilton Rodrigues Collere, Flávia Carolina Meira Vieira, Thállitha Samih Wischral Jayme Pereira, Luiz Cezar Machado Nicola, Patricia Avello Vieira, Rafael Felipe da Costa Horta, Mauricio Claudio Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil. |
topic_facet |
16S rRNA 23S rRNA Hemotropic mycoplasmas Sequencing qPCR |
description |
A total of 231 blood samples from wild mammals belonging to the orders Rodentia (n = 142) and Didelphimorphia (n = 89) were screened by real-time PCR assay (qPCR), being six Rhipidomys sp., 118 Thrichomys laurentius, nine Rattus rattus, four Kerodon rupestris, five Necromys lasiurus, 42 Didelphis albiventris and 47 Monodelphis domestica. Results using qPCR showed that 32 of the total 231 (13.85 %) samples were positive for hemoplasma sequences of the 16S rRNA gene. Sequences from two D. albiventris showed 99.77-99.89 % identity with 'Candidatus Mycoplasma haemoalbiventris' and 99.09 % with 'Candidatus Mycoplasma haemodidelphidis', respectively. Furthermore, one M. domestica and five T. laurentius showed 99.72-99.77 % identity with Mycoplasma sp., and one K. rupestris showed 98.13 % identity with 'Candidatus Mycoplasma haematohydrochaerus'; and from two Rattus rattus showed 99.65-99.89 % identity with Mycoplasma sp. and 'Candidatus Mycoplasma haemomuris'. The 23S rRNA gene sequences obtained from the two D. albiventris showed 100 % identity with 'Ca. M. haemoalbiventris' whereas the sequences from the R. rattus showed only 85.31 % identity with 'Candidatus Mycoplasma haematohydrochaerus'. Two T. laurentius and one K. rupestris showed 84.66-92.97 % identity with 'Candidatus Mycoplasma haemosphiggurus'. Based on phylogenetic and Neighbor-Net network analyses of the 16S and 23S rRNA genes, potential novel species are described. In addition, 'Ca. M. haemoalbiventris' was detected in Didelphis albiventris, and Mycoplasma sp. was detected in Rattus sp. rodents from the Caatinga biome, Brazil. |
format |
Article in Journal/Newspaper |
author |
Torres-Santos, Paula Talita Evaristo, Anna Maria da Cruz Ferreira Santos, Josenilton Rodrigues Collere, Flávia Carolina Meira Vieira, Thállitha Samih Wischral Jayme Pereira, Luiz Cezar Machado Nicola, Patricia Avello Vieira, Rafael Felipe da Costa Horta, Mauricio Claudio |
author_facet |
Torres-Santos, Paula Talita Evaristo, Anna Maria da Cruz Ferreira Santos, Josenilton Rodrigues Collere, Flávia Carolina Meira Vieira, Thállitha Samih Wischral Jayme Pereira, Luiz Cezar Machado Nicola, Patricia Avello Vieira, Rafael Felipe da Costa Horta, Mauricio Claudio |
author_sort |
Torres-Santos, Paula Talita |
title |
Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil. |
title_short |
Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil. |
title_full |
Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil. |
title_fullStr |
Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil. |
title_full_unstemmed |
Hemoplasmas in wild rodents and marsupials from the Caatinga Biome, Brazil. |
title_sort |
hemoplasmas in wild rodents and marsupials from the caatinga biome, brazil. |
publisher |
Elsevier Science |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.actatropica.2024.107334 https://pubmed.ncbi.nlm.nih.gov/39127138 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Acta Trop ISSN:1873-6254 Volume:258 |
op_relation |
https://doi.org/10.1016/j.actatropica.2024.107334 https://pubmed.ncbi.nlm.nih.gov/39127138 |
op_rights |
Copyright © 2024. Published by Elsevier B.V. |
op_doi |
https://doi.org/10.1016/j.actatropica.2024.107334 |
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Acta Tropica |
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258 |
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107334 |
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1811644187738636288 |