Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility.
PIWI-interacting RNAs (piRNAs) are crucial for silencing transposable elements, germ cell development, and gametogenesis. Triploid Pacific oysters (Crassostrea gigas) are vital in the oyster aquaculture industry due to reduced fertility and rapid growth. This study integrates piRNA and mRNA expressi...
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ftpubmed:39073646 2024-09-09T19:37:22+00:00 Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility. Zhou, Yaru Yu, Hong Li, Qi Kong, Lingfeng Liu, Shikai Xu, Chengxun 2024 Jul 29 https://doi.org/10.1007/s10126-024-10351-7 https://pubmed.ncbi.nlm.nih.gov/39073646 eng eng Springer https://doi.org/10.1007/s10126-024-10351-7 https://pubmed.ncbi.nlm.nih.gov/39073646 © 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. Mar Biotechnol (NY) ISSN:1436-2236 Crassostrea gigas Sterility Triplod piRNA piRNA-mRNA interaction Journal Article 2024 ftpubmed https://doi.org/10.1007/s10126-024-10351-7 2024-07-29T16:03:00Z PIWI-interacting RNAs (piRNAs) are crucial for silencing transposable elements, germ cell development, and gametogenesis. Triploid Pacific oysters (Crassostrea gigas) are vital in the oyster aquaculture industry due to reduced fertility and rapid growth. This study integrates piRNA and mRNA expression analyses to elucidate their potential contributions to the sterility of triploid C. gigas. Bioinformatics analysis reveals a distinct U-bias at the 5' terminal of oyster piRNAs. The abundance of piRNA clusters is reduced in triploid gonads compared to diploid gonads, particularly in sterile gonads, with a significant decrease in piRNA numbers. A specific piRNA cluster is annotated with the PPP4R1 gene, which is downregulated in infertile female triploids and exhibits a negative correlation with three piRNAs within the cluster. Differential expression analysis identified 46 and 88 piRNAs in female and male comparison groups, respectively. In female sterile triploids, the expression of three target genes of differentially expressed piRNAs associated with cell division showed downregulation, suggesting the potential roles of piRNAs in the regulation of cell division-related genes, contributing to the gonad arrest observed in female triploid oysters. In male triploid oysters, piRNAs potentially interact with the target genes associated with spermatogenesis, including TSSK4, SPAG17, and CCDC81. This study provides a concise overview of piRNAs expression in oyster gonads, offering insights into the regulatory role of piRNAs in triploid sterility. Article in Journal/Newspaper Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Marine Biotechnology |
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Open Polar |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Crassostrea gigas Sterility Triplod piRNA piRNA-mRNA interaction |
spellingShingle |
Crassostrea gigas Sterility Triplod piRNA piRNA-mRNA interaction Zhou, Yaru Yu, Hong Li, Qi Kong, Lingfeng Liu, Shikai Xu, Chengxun Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility. |
topic_facet |
Crassostrea gigas Sterility Triplod piRNA piRNA-mRNA interaction |
description |
PIWI-interacting RNAs (piRNAs) are crucial for silencing transposable elements, germ cell development, and gametogenesis. Triploid Pacific oysters (Crassostrea gigas) are vital in the oyster aquaculture industry due to reduced fertility and rapid growth. This study integrates piRNA and mRNA expression analyses to elucidate their potential contributions to the sterility of triploid C. gigas. Bioinformatics analysis reveals a distinct U-bias at the 5' terminal of oyster piRNAs. The abundance of piRNA clusters is reduced in triploid gonads compared to diploid gonads, particularly in sterile gonads, with a significant decrease in piRNA numbers. A specific piRNA cluster is annotated with the PPP4R1 gene, which is downregulated in infertile female triploids and exhibits a negative correlation with three piRNAs within the cluster. Differential expression analysis identified 46 and 88 piRNAs in female and male comparison groups, respectively. In female sterile triploids, the expression of three target genes of differentially expressed piRNAs associated with cell division showed downregulation, suggesting the potential roles of piRNAs in the regulation of cell division-related genes, contributing to the gonad arrest observed in female triploid oysters. In male triploid oysters, piRNAs potentially interact with the target genes associated with spermatogenesis, including TSSK4, SPAG17, and CCDC81. This study provides a concise overview of piRNAs expression in oyster gonads, offering insights into the regulatory role of piRNAs in triploid sterility. |
format |
Article in Journal/Newspaper |
author |
Zhou, Yaru Yu, Hong Li, Qi Kong, Lingfeng Liu, Shikai Xu, Chengxun |
author_facet |
Zhou, Yaru Yu, Hong Li, Qi Kong, Lingfeng Liu, Shikai Xu, Chengxun |
author_sort |
Zhou, Yaru |
title |
Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility. |
title_short |
Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility. |
title_full |
Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility. |
title_fullStr |
Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility. |
title_full_unstemmed |
Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility. |
title_sort |
characterization of pirnas in diploid and triploid pacific oyster gonads: exploring their potential roles in triploid sterility. |
publisher |
Springer |
publishDate |
2024 |
url |
https://doi.org/10.1007/s10126-024-10351-7 https://pubmed.ncbi.nlm.nih.gov/39073646 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Mar Biotechnol (NY) ISSN:1436-2236 |
op_relation |
https://doi.org/10.1007/s10126-024-10351-7 https://pubmed.ncbi.nlm.nih.gov/39073646 |
op_rights |
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. |
op_doi |
https://doi.org/10.1007/s10126-024-10351-7 |
container_title |
Marine Biotechnology |
_version_ |
1809906542809448448 |