Whole-genome sequencing of 13 Arctic plants and draft genomes of Oxyria digyna and Cochlearia groenlandica.

• Published in: Scientific Data
• Main Authors: Kim, Jun, Lim, Jiseon, Kim, Moonkyo, Lee, Yoo Kyung
• Format: Dataset
• Language: English
• Published: Nature Publishing Group 2024
• Subjects: Cochlearia groenlandica; Greenland; Greenland scurvy-grass; Oxyria digyna; Svalbard
• Online Access: https://doi.org/10.1038/s41597-024-03569-6; https://pubmed.ncbi.nlm.nih.gov/39025921; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11258133/

To understand the genomic characteristics of Arctic plants, we generated 28-44 Gb of short-read sequencing data from 13 Arctic plants collected from the High Arctic Svalbard. We successfully estimated the genome sizes of eight species by using the k-mer-based method (180-894 Mb). Among these plants, the mountain sorrel (Oxyria digyna) and Greenland scurvy grass (Cochlearia groenlandica) had relatively small genome sizes and chromosome numbers. We obtained 45 × and 121 × high-fidelity long-read sequencing data. We assembled their reads into high-quality draft genomes (genome size: 561 and 250 Mb; contig N50 length: 36.9 and 14.8 Mb, respectively), and correspondingly annotated 43,105 and 29,675 genes using ~46 and ~85 million RNA sequencing reads. We identified 765,012 and 88,959 single-nucleotide variants, and 18,082 and 7,698 structural variants (variant size ≥ 50 bp). This study provided high-quality genome assemblies of O. digyna and C. groenlandica, which are valuable resources for the population and molecular genetic studies of these plants.