Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems.
Passive samplers are enabling the scaling of environmental DNA (eDNA) biomonitoring in our oceans, by circumventing the time-consuming process of water filtration. Designing a novel passive sampler that does not require extensive sample handling time and can be connected to ocean-going vessels witho...
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ftpubmed:38955269 2024-09-15T17:48:35+00:00 Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems. Jeunen, Gert-Jan Mills, Sadie Mariani, Stefano Treece, Jackson Ferreira, Sara Stanton, Jo-Ann L Durán-Vinet, Benjamín Duffy, Grant A Gemmell, Neil J Lamare, Miles 2024 Oct 10 https://doi.org/10.1016/j.scitotenv.2024.174354 https://pubmed.ncbi.nlm.nih.gov/38955269 eng eng Elsevier Science https://doi.org/10.1016/j.scitotenv.2024.174354 https://pubmed.ncbi.nlm.nih.gov/38955269 Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved. Sci Total Environ ISSN:1879-1026 Volume:946 Antarctica Artificial sponges COI metabarcoding Environmental DNA Southern Ocean Journal Article 2024 ftpubmed https://doi.org/10.1016/j.scitotenv.2024.174354 2024-07-20T16:02:00Z Passive samplers are enabling the scaling of environmental DNA (eDNA) biomonitoring in our oceans, by circumventing the time-consuming process of water filtration. Designing a novel passive sampler that does not require extensive sample handling time and can be connected to ocean-going vessels without impeding normal underway activities has potential to rapidly upscale global biomonitoring efforts onboard the world's oceanic fleet. Here, we demonstrate the utility of an artificial sponge sampler connected to the continuous pump underway seawater system as a means to enable oceanic biomonitoring. We compared the performance of this passive sampling protocol with standard water filtration at six locations during a research voyage from New Zealand to Antarctica in early 2023. Eukaryote metabarcoding of the mitochondrial COI gene revealed no significant difference in phylogenetic α-diversity between sampling methods and both methods delineated a progressive reduction in number of Zero-Radius Operational Taxonomic Units (ZOTUs) with increased latitudes. While both sampling methods revealed comparable trends in geographical community compositions, distinct clusters were identified for passive samplers and water filtration at each location. Additionally, greater variability between replicates was observed for passive samplers, resulting in an increased estimated level of replication needed to recover 90 % of the biodiversity. Furthermore, traditional water filtration failed to detect three phyla observed by passive samplers and extrapolation analysis estimated passive samplers recover a larger number of ZOTUs compared to water filtration for all six locations. Our results demonstrate the potential of this passive eDNA sampler protocol and highlight areas where this emerging technology could be improved, thereby enabling large-scale offshore marine eDNA biomonitoring by leveraging the world's oceanic fleet without interfering with onboard activities. Article in Journal/Newspaper Antarc* Antarctica Southern Ocean PubMed Central (PMC) Science of The Total Environment 946 174354 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Antarctica Artificial sponges COI metabarcoding Environmental DNA Southern Ocean |
spellingShingle |
Antarctica Artificial sponges COI metabarcoding Environmental DNA Southern Ocean Jeunen, Gert-Jan Mills, Sadie Mariani, Stefano Treece, Jackson Ferreira, Sara Stanton, Jo-Ann L Durán-Vinet, Benjamín Duffy, Grant A Gemmell, Neil J Lamare, Miles Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems. |
topic_facet |
Antarctica Artificial sponges COI metabarcoding Environmental DNA Southern Ocean |
description |
Passive samplers are enabling the scaling of environmental DNA (eDNA) biomonitoring in our oceans, by circumventing the time-consuming process of water filtration. Designing a novel passive sampler that does not require extensive sample handling time and can be connected to ocean-going vessels without impeding normal underway activities has potential to rapidly upscale global biomonitoring efforts onboard the world's oceanic fleet. Here, we demonstrate the utility of an artificial sponge sampler connected to the continuous pump underway seawater system as a means to enable oceanic biomonitoring. We compared the performance of this passive sampling protocol with standard water filtration at six locations during a research voyage from New Zealand to Antarctica in early 2023. Eukaryote metabarcoding of the mitochondrial COI gene revealed no significant difference in phylogenetic α-diversity between sampling methods and both methods delineated a progressive reduction in number of Zero-Radius Operational Taxonomic Units (ZOTUs) with increased latitudes. While both sampling methods revealed comparable trends in geographical community compositions, distinct clusters were identified for passive samplers and water filtration at each location. Additionally, greater variability between replicates was observed for passive samplers, resulting in an increased estimated level of replication needed to recover 90 % of the biodiversity. Furthermore, traditional water filtration failed to detect three phyla observed by passive samplers and extrapolation analysis estimated passive samplers recover a larger number of ZOTUs compared to water filtration for all six locations. Our results demonstrate the potential of this passive eDNA sampler protocol and highlight areas where this emerging technology could be improved, thereby enabling large-scale offshore marine eDNA biomonitoring by leveraging the world's oceanic fleet without interfering with onboard activities. |
format |
Article in Journal/Newspaper |
author |
Jeunen, Gert-Jan Mills, Sadie Mariani, Stefano Treece, Jackson Ferreira, Sara Stanton, Jo-Ann L Durán-Vinet, Benjamín Duffy, Grant A Gemmell, Neil J Lamare, Miles |
author_facet |
Jeunen, Gert-Jan Mills, Sadie Mariani, Stefano Treece, Jackson Ferreira, Sara Stanton, Jo-Ann L Durán-Vinet, Benjamín Duffy, Grant A Gemmell, Neil J Lamare, Miles |
author_sort |
Jeunen, Gert-Jan |
title |
Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems. |
title_short |
Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems. |
title_full |
Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems. |
title_fullStr |
Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems. |
title_full_unstemmed |
Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems. |
title_sort |
streamlining large-scale oceanic biomonitoring using passive edna samplers integrated into vessel's continuous pump underway seawater systems. |
publisher |
Elsevier Science |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.scitotenv.2024.174354 https://pubmed.ncbi.nlm.nih.gov/38955269 |
genre |
Antarc* Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctica Southern Ocean |
op_source |
Sci Total Environ ISSN:1879-1026 Volume:946 |
op_relation |
https://doi.org/10.1016/j.scitotenv.2024.174354 https://pubmed.ncbi.nlm.nih.gov/38955269 |
op_rights |
Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.scitotenv.2024.174354 |
container_title |
Science of The Total Environment |
container_volume |
946 |
container_start_page |
174354 |
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1810289977639043072 |