Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems.
Actinomycetes are a phylogenetically diverse bacterial group which are widely distributed across terrestrial and aquatic ecosystems. Within this order, the genus Pseudonocardia and their specialised metabolites have been the focus of previous ecological studies due to their antagonistic interactions...
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Online Access: | https://doi.org/10.1111/1462-2920.16635 https://pubmed.ncbi.nlm.nih.gov/38899724 |
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ftpubmed:38899724 2024-09-15T18:37:17+00:00 Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. Parra, Jonathan Jarmusch, Scott A Duncan, Katherine R 2024 Jun https://doi.org/10.1111/1462-2920.16635 https://pubmed.ncbi.nlm.nih.gov/38899724 eng eng Wiley https://doi.org/10.1111/1462-2920.16635 https://pubmed.ncbi.nlm.nih.gov/38899724 © 2024 The Author(s). Environmental Microbiology published by John Wiley & Sons Ltd. Environ Microbiol ISSN:1462-2920 Volume:26 Issue:6 Journal Article 2024 ftpubmed https://doi.org/10.1111/1462-2920.16635 2024-07-20T16:02:00Z Actinomycetes are a phylogenetically diverse bacterial group which are widely distributed across terrestrial and aquatic ecosystems. Within this order, the genus Pseudonocardia and their specialised metabolites have been the focus of previous ecological studies due to their antagonistic interactions with other microorganisms and their mutualistic interactions with insects. However, the chemical ecology of free-living Pseudonocardia remains understudied. This study applies a multi-omics approach to investigate the chemical ecology of free-living actinomycetes from the genus Pseudonocardia. In a comparative genomics analysis, it was observed that the biosynthetic gene cluster family distribution was influenced mainly by phylogenetic distance rather than the geographic or ecological origin of strains. This finding was also observed in the mass spectrometry-based metabolomic profiles of nine Pseudonocardia species isolated from marine sediments and two terrestrial species. Antagonist interactions between these 11 species were examined, and matrix-assisted laser desorption/ionisation-mass spectrometry imaging was used to examine in situ chemical interactions between the Southern Ocean strains and their phylogenetically close relatives. Overall, it was demonstrated that phylogeny was the main predictor of antagonistic interactions among free-living Pseudonocardia. Moreover, two features at m/z 441.15 and m/z 332.20 were identified as metabolites related to these interspecies interactions. Article in Journal/Newspaper Southern Ocean PubMed Central (PMC) Environmental Microbiology 26 6 |
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English |
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Actinomycetes are a phylogenetically diverse bacterial group which are widely distributed across terrestrial and aquatic ecosystems. Within this order, the genus Pseudonocardia and their specialised metabolites have been the focus of previous ecological studies due to their antagonistic interactions with other microorganisms and their mutualistic interactions with insects. However, the chemical ecology of free-living Pseudonocardia remains understudied. This study applies a multi-omics approach to investigate the chemical ecology of free-living actinomycetes from the genus Pseudonocardia. In a comparative genomics analysis, it was observed that the biosynthetic gene cluster family distribution was influenced mainly by phylogenetic distance rather than the geographic or ecological origin of strains. This finding was also observed in the mass spectrometry-based metabolomic profiles of nine Pseudonocardia species isolated from marine sediments and two terrestrial species. Antagonist interactions between these 11 species were examined, and matrix-assisted laser desorption/ionisation-mass spectrometry imaging was used to examine in situ chemical interactions between the Southern Ocean strains and their phylogenetically close relatives. Overall, it was demonstrated that phylogeny was the main predictor of antagonistic interactions among free-living Pseudonocardia. Moreover, two features at m/z 441.15 and m/z 332.20 were identified as metabolites related to these interspecies interactions. |
format |
Article in Journal/Newspaper |
author |
Parra, Jonathan Jarmusch, Scott A Duncan, Katherine R |
spellingShingle |
Parra, Jonathan Jarmusch, Scott A Duncan, Katherine R Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. |
author_facet |
Parra, Jonathan Jarmusch, Scott A Duncan, Katherine R |
author_sort |
Parra, Jonathan |
title |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. |
title_short |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. |
title_full |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. |
title_fullStr |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. |
title_full_unstemmed |
Multi-omics analysis of antagonistic interactions among free-living Pseudonocardia from diverse ecosystems. |
title_sort |
multi-omics analysis of antagonistic interactions among free-living pseudonocardia from diverse ecosystems. |
publisher |
Wiley |
publishDate |
2024 |
url |
https://doi.org/10.1111/1462-2920.16635 https://pubmed.ncbi.nlm.nih.gov/38899724 |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Environ Microbiol ISSN:1462-2920 Volume:26 Issue:6 |
op_relation |
https://doi.org/10.1111/1462-2920.16635 https://pubmed.ncbi.nlm.nih.gov/38899724 |
op_rights |
© 2024 The Author(s). Environmental Microbiology published by John Wiley & Sons Ltd. |
op_doi |
https://doi.org/10.1111/1462-2920.16635 |
container_title |
Environmental Microbiology |
container_volume |
26 |
container_issue |
6 |
_version_ |
1810481637162483712 |