A novel surface marker CD49d promotes TNF expression in oyster agranulocytes by mediating the MAPK pathway.
CD49d, encoded by the gene Integrin α4, is a significant member of cell adhesion receptors, which is widely expressed in various immune cells to trigger immune responses against invading pathogens. In the present study, the expression of CgCD49d and its regulatory role in TNF expression were investi...
Published in: | Fish & Shellfish Immunology |
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ftpubmed:38897309 2024-09-15T18:03:09+00:00 A novel surface marker CD49d promotes TNF expression in oyster agranulocytes by mediating the MAPK pathway. Dong, Miren Wang, Weilin Wu, Wei Cheng, Xuemei Cheng, Junlei Wang, Lingling Song, Linsheng 2024 Aug https://doi.org/10.1016/j.fsi.2024.109702 https://pubmed.ncbi.nlm.nih.gov/38897309 eng eng Elsevier Science https://doi.org/10.1016/j.fsi.2024.109702 https://pubmed.ncbi.nlm.nih.gov/38897309 Copyright © 2024. Published by Elsevier Ltd. Fish Shellfish Immunol ISSN:1095-9947 Volume:151 Agranulocytes CD49d Crassostrea gigas MAPK/JNK pathway TNF Journal Article 2024 ftpubmed https://doi.org/10.1016/j.fsi.2024.109702 2024-07-22T16:02:00Z CD49d, encoded by the gene Integrin α4, is a significant member of cell adhesion receptors, which is widely expressed in various immune cells to trigger immune responses against invading pathogens. In the present study, the expression of CgCD49d and its regulatory role in TNF expression were investigated in the Pacific oyster Crassostrea gigas. There were five Int-alpha domains, an Integrin_alpha2 region and a unique FG-GAP repeat region inserted identified in CgCD49d. CgCD49d transcript was specifically expressed in haemocytes, and its mRNA expression level in haemocytes increased after LPS and Vibrio splendidus stimulation. After CgCD49d was blocked by using its antibody, the phosphorylation level of CgJNK in the MAPK signaling pathway and CgTNF transcripts decreased significantly post V. splendidus stimulation. After phosphorylation level of CgJNK was inhibited by using its inhibitor, the nuclear translocation of CgRel was restrained and CgTNF transcripts also decreased significantly post V. splendidus stimulation. Furthermore, CgCD49d was found to be mainly expressed in the agranulocyte subpopulation, and Alexa Fluor 488-conjugated CgCD49d antibody labeled agranulocytes with a circle of green fluorescence signals on CgCD49d+ agranulocyte surface under Confocal microscopy, which accounted for 24.9 ± 4.53% of total haemocytes. Collectively, these results suggested that CgCD49d promoted TNF expression in oyster haemocytes against bacterial invasion by mediating MAPK pathway, and it could be used as a surface marker to type and sort a subset of agranulocyte subpopulation among haemocytes. Article in Journal/Newspaper Crassostrea gigas Pacific oyster PubMed Central (PMC) Fish & Shellfish Immunology 151 109702 |
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Open Polar |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Agranulocytes CD49d Crassostrea gigas MAPK/JNK pathway TNF |
spellingShingle |
Agranulocytes CD49d Crassostrea gigas MAPK/JNK pathway TNF Dong, Miren Wang, Weilin Wu, Wei Cheng, Xuemei Cheng, Junlei Wang, Lingling Song, Linsheng A novel surface marker CD49d promotes TNF expression in oyster agranulocytes by mediating the MAPK pathway. |
topic_facet |
Agranulocytes CD49d Crassostrea gigas MAPK/JNK pathway TNF |
description |
CD49d, encoded by the gene Integrin α4, is a significant member of cell adhesion receptors, which is widely expressed in various immune cells to trigger immune responses against invading pathogens. In the present study, the expression of CgCD49d and its regulatory role in TNF expression were investigated in the Pacific oyster Crassostrea gigas. There were five Int-alpha domains, an Integrin_alpha2 region and a unique FG-GAP repeat region inserted identified in CgCD49d. CgCD49d transcript was specifically expressed in haemocytes, and its mRNA expression level in haemocytes increased after LPS and Vibrio splendidus stimulation. After CgCD49d was blocked by using its antibody, the phosphorylation level of CgJNK in the MAPK signaling pathway and CgTNF transcripts decreased significantly post V. splendidus stimulation. After phosphorylation level of CgJNK was inhibited by using its inhibitor, the nuclear translocation of CgRel was restrained and CgTNF transcripts also decreased significantly post V. splendidus stimulation. Furthermore, CgCD49d was found to be mainly expressed in the agranulocyte subpopulation, and Alexa Fluor 488-conjugated CgCD49d antibody labeled agranulocytes with a circle of green fluorescence signals on CgCD49d+ agranulocyte surface under Confocal microscopy, which accounted for 24.9 ± 4.53% of total haemocytes. Collectively, these results suggested that CgCD49d promoted TNF expression in oyster haemocytes against bacterial invasion by mediating MAPK pathway, and it could be used as a surface marker to type and sort a subset of agranulocyte subpopulation among haemocytes. |
format |
Article in Journal/Newspaper |
author |
Dong, Miren Wang, Weilin Wu, Wei Cheng, Xuemei Cheng, Junlei Wang, Lingling Song, Linsheng |
author_facet |
Dong, Miren Wang, Weilin Wu, Wei Cheng, Xuemei Cheng, Junlei Wang, Lingling Song, Linsheng |
author_sort |
Dong, Miren |
title |
A novel surface marker CD49d promotes TNF expression in oyster agranulocytes by mediating the MAPK pathway. |
title_short |
A novel surface marker CD49d promotes TNF expression in oyster agranulocytes by mediating the MAPK pathway. |
title_full |
A novel surface marker CD49d promotes TNF expression in oyster agranulocytes by mediating the MAPK pathway. |
title_fullStr |
A novel surface marker CD49d promotes TNF expression in oyster agranulocytes by mediating the MAPK pathway. |
title_full_unstemmed |
A novel surface marker CD49d promotes TNF expression in oyster agranulocytes by mediating the MAPK pathway. |
title_sort |
novel surface marker cd49d promotes tnf expression in oyster agranulocytes by mediating the mapk pathway. |
publisher |
Elsevier Science |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.fsi.2024.109702 https://pubmed.ncbi.nlm.nih.gov/38897309 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Fish Shellfish Immunol ISSN:1095-9947 Volume:151 |
op_relation |
https://doi.org/10.1016/j.fsi.2024.109702 https://pubmed.ncbi.nlm.nih.gov/38897309 |
op_rights |
Copyright © 2024. Published by Elsevier Ltd. |
op_doi |
https://doi.org/10.1016/j.fsi.2024.109702 |
container_title |
Fish & Shellfish Immunology |
container_volume |
151 |
container_start_page |
109702 |
_version_ |
1810440674435137536 |