Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers.
In recent years micro- and nanoplastics and metal-oxide nanomaterials have been found in several environmental compartments. The Antarctic soft clam Laternula elliptica is an endemic Antarctic species having a wide distribution in the Southern Ocean. Being a filter-feeder, it could act as suitable b...
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Online Access: | https://doi.org/10.1016/j.ecoenv.2024.116523 https://pubmed.ncbi.nlm.nih.gov/38850707 |
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ftpubmed:38850707 2024-09-09T19:12:07+00:00 Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers. Rondon, Rodolfo Valdés, Catalina Cosseau, Céline Bergami, Elisa Cárdenas, César Antonio Balbi, Teresa Pérez-Toledo, Carolina Garrido, Ignacio Perrois, Garance Chaparro, Cristian Corre, Erwan Corsi, Ilaria González-Aravena, Marcelo 2024 Jul 15 https://doi.org/10.1016/j.ecoenv.2024.116523 https://pubmed.ncbi.nlm.nih.gov/38850707 eng eng Elsevier Science https://doi.org/10.1016/j.ecoenv.2024.116523 https://pubmed.ncbi.nlm.nih.gov/38850707 Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved. Ecotoxicol Environ Saf ISSN:1090-2414 Volume:280 Antarctic benthos Biomarkers Gene expression Nanomaterials Nanoplastic Toxicity Journal Article 2024 ftpubmed https://doi.org/10.1016/j.ecoenv.2024.116523 2024-06-24T16:01:00Z In recent years micro- and nanoplastics and metal-oxide nanomaterials have been found in several environmental compartments. The Antarctic soft clam Laternula elliptica is an endemic Antarctic species having a wide distribution in the Southern Ocean. Being a filter-feeder, it could act as suitable bioindicator of pollution from nanoparticles also considering its sensitivity to various sources of stress. The present study aims to assess the impact of polystyrene nanoparticles (PS-NP) and the nanometal titanium-dioxide (n-TiO2) on genome-wide transcript expression of L. elliptica either alone and in combination and at two toxicological relevant concentrations (5 and 50 µg/L) during 96 h exposure. Transcript-target qRT-PCR was performed with the aim to identify suitable biomarkers of exposure and effects. As expected, at the highest concentration tested, the clustering was clearer between control and exposed clams. A total of 221 genes resulted differentially expressed in exposed clams and control ones, and 21 of them had functional annotation such as ribosomal proteins, antioxidant, ion transport (osmoregulation), acid-base balance, immunity, lipid metabolism, cell adhesion, cytoskeleton, apoptosis, chromatin condensation and cell signaling. At functional level, relevant transcripts were shared among some treatments and could be considered as general stress due to nanoparticle exposure. After applying transcript-target approach duplicating the number of clam samples, four ecologically relevant transcripts were revealed as biomarkers for PS-NP, n-TiO2 and their combination at 50 µg/L, that could be used for monitoring clams' health status in different Antarctic localities. Article in Journal/Newspaper Antarc* Antarctic Southern Ocean PubMed Central (PMC) Antarctic Southern Ocean The Antarctic Ecotoxicology and Environmental Safety 280 116523 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Antarctic benthos Biomarkers Gene expression Nanomaterials Nanoplastic Toxicity |
spellingShingle |
Antarctic benthos Biomarkers Gene expression Nanomaterials Nanoplastic Toxicity Rondon, Rodolfo Valdés, Catalina Cosseau, Céline Bergami, Elisa Cárdenas, César Antonio Balbi, Teresa Pérez-Toledo, Carolina Garrido, Ignacio Perrois, Garance Chaparro, Cristian Corre, Erwan Corsi, Ilaria González-Aravena, Marcelo Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers. |
topic_facet |
Antarctic benthos Biomarkers Gene expression Nanomaterials Nanoplastic Toxicity |
description |
In recent years micro- and nanoplastics and metal-oxide nanomaterials have been found in several environmental compartments. The Antarctic soft clam Laternula elliptica is an endemic Antarctic species having a wide distribution in the Southern Ocean. Being a filter-feeder, it could act as suitable bioindicator of pollution from nanoparticles also considering its sensitivity to various sources of stress. The present study aims to assess the impact of polystyrene nanoparticles (PS-NP) and the nanometal titanium-dioxide (n-TiO2) on genome-wide transcript expression of L. elliptica either alone and in combination and at two toxicological relevant concentrations (5 and 50 µg/L) during 96 h exposure. Transcript-target qRT-PCR was performed with the aim to identify suitable biomarkers of exposure and effects. As expected, at the highest concentration tested, the clustering was clearer between control and exposed clams. A total of 221 genes resulted differentially expressed in exposed clams and control ones, and 21 of them had functional annotation such as ribosomal proteins, antioxidant, ion transport (osmoregulation), acid-base balance, immunity, lipid metabolism, cell adhesion, cytoskeleton, apoptosis, chromatin condensation and cell signaling. At functional level, relevant transcripts were shared among some treatments and could be considered as general stress due to nanoparticle exposure. After applying transcript-target approach duplicating the number of clam samples, four ecologically relevant transcripts were revealed as biomarkers for PS-NP, n-TiO2 and their combination at 50 µg/L, that could be used for monitoring clams' health status in different Antarctic localities. |
format |
Article in Journal/Newspaper |
author |
Rondon, Rodolfo Valdés, Catalina Cosseau, Céline Bergami, Elisa Cárdenas, César Antonio Balbi, Teresa Pérez-Toledo, Carolina Garrido, Ignacio Perrois, Garance Chaparro, Cristian Corre, Erwan Corsi, Ilaria González-Aravena, Marcelo |
author_facet |
Rondon, Rodolfo Valdés, Catalina Cosseau, Céline Bergami, Elisa Cárdenas, César Antonio Balbi, Teresa Pérez-Toledo, Carolina Garrido, Ignacio Perrois, Garance Chaparro, Cristian Corre, Erwan Corsi, Ilaria González-Aravena, Marcelo |
author_sort |
Rondon, Rodolfo |
title |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers. |
title_short |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers. |
title_full |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers. |
title_fullStr |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers. |
title_full_unstemmed |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers. |
title_sort |
transcriptomic responses of antarctic clam laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers. |
publisher |
Elsevier Science |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.ecoenv.2024.116523 https://pubmed.ncbi.nlm.nih.gov/38850707 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_source |
Ecotoxicol Environ Saf ISSN:1090-2414 Volume:280 |
op_relation |
https://doi.org/10.1016/j.ecoenv.2024.116523 https://pubmed.ncbi.nlm.nih.gov/38850707 |
op_rights |
Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.ecoenv.2024.116523 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
280 |
container_start_page |
116523 |
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1809752655465021440 |