Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing.
Springtails (subclass: Collembola) represent one of the most extensively studied invertebrate groups in soil ecotoxicology. This is because of their ease of laboratory culture, significant ecological role, and sensitivity to environmental contaminants. Folsomia candida (family: Isotomidae) is a glob...
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ftpubmed:38837715 2024-09-15T18:41:50+00:00 Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing. Martin, William J Sibley, Paul K Prosser, Ryan S 2024 Aug https://doi.org/10.1002/etc.5898 https://pubmed.ncbi.nlm.nih.gov/38837715 eng eng Wiley https://doi.org/10.1002/etc.5898 https://pubmed.ncbi.nlm.nih.gov/38837715 © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Environ Toxicol Chem ISSN:1552-8618 Volume:43 Issue:8 Pesticides Soil ecotoxicology Soil invertebrates Terrestrial invertebrate toxicology Journal Article 2024 ftpubmed https://doi.org/10.1002/etc.5898 2024-07-26T16:03:00Z Springtails (subclass: Collembola) represent one of the most extensively studied invertebrate groups in soil ecotoxicology. This is because of their ease of laboratory culture, significant ecological role, and sensitivity to environmental contaminants. Folsomia candida (family: Isotomidae) is a globally widespread parthenogenetic species that is prevalent in laboratory toxicity testing with springtails. Conversely, Arrhopalites caecus (family: Arrhopalitidae), a parthenogenic globular springtail species, remains untested in soil ecotoxicology. This species is found in diverse habitats, including cave systems and forest leaf litter, and has a global distribution. The sensitivity of A. caecus to environmental contaminants, such as neonicotinoid insecticides, as well as its life history and optimal culturing conditions, are largely unknown. The present study describes the establishment of a pure A. caecus laboratory culture and characterization of its life cycle and culturing conditions. We assessed the sensitivity of A. caecus to various insecticides, including exposures to the neonicotinoid thiamethoxam in soil and through a novel feeding assay as well as to clothianidin and cyantraniliprole in spiked soil exposures. In 7- and 14-day exposures to thiamethoxam in agricultural soil, the 50% lethal concentration (LC50) values were determined to be 0.129 mg/kg dry weight and 0.010 mg/kg dry weight, respectively. The 14-day LC50 for exposure to thiamethoxam via spiked food was determined to be 0.307 mg/kg dry weight. In addition, the 28-day 50% effect concentration for inhibition of juvenile production from cyantraniliprole exposure in the same soil type was 0.055 mg/kg dry weight. Challenges encountered in using this species included susceptibility to mite infestation and low adult survival rates in the 28-day cyantraniliprole test. Environ Toxicol Chem 2024;43:1820-1835. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Article in Journal/Newspaper Mite Springtail PubMed Central (PMC) Environmental Toxicology and Chemistry 43 8 1820 1835 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Pesticides Soil ecotoxicology Soil invertebrates Terrestrial invertebrate toxicology |
spellingShingle |
Pesticides Soil ecotoxicology Soil invertebrates Terrestrial invertebrate toxicology Martin, William J Sibley, Paul K Prosser, Ryan S Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing. |
topic_facet |
Pesticides Soil ecotoxicology Soil invertebrates Terrestrial invertebrate toxicology |
description |
Springtails (subclass: Collembola) represent one of the most extensively studied invertebrate groups in soil ecotoxicology. This is because of their ease of laboratory culture, significant ecological role, and sensitivity to environmental contaminants. Folsomia candida (family: Isotomidae) is a globally widespread parthenogenetic species that is prevalent in laboratory toxicity testing with springtails. Conversely, Arrhopalites caecus (family: Arrhopalitidae), a parthenogenic globular springtail species, remains untested in soil ecotoxicology. This species is found in diverse habitats, including cave systems and forest leaf litter, and has a global distribution. The sensitivity of A. caecus to environmental contaminants, such as neonicotinoid insecticides, as well as its life history and optimal culturing conditions, are largely unknown. The present study describes the establishment of a pure A. caecus laboratory culture and characterization of its life cycle and culturing conditions. We assessed the sensitivity of A. caecus to various insecticides, including exposures to the neonicotinoid thiamethoxam in soil and through a novel feeding assay as well as to clothianidin and cyantraniliprole in spiked soil exposures. In 7- and 14-day exposures to thiamethoxam in agricultural soil, the 50% lethal concentration (LC50) values were determined to be 0.129 mg/kg dry weight and 0.010 mg/kg dry weight, respectively. The 14-day LC50 for exposure to thiamethoxam via spiked food was determined to be 0.307 mg/kg dry weight. In addition, the 28-day 50% effect concentration for inhibition of juvenile production from cyantraniliprole exposure in the same soil type was 0.055 mg/kg dry weight. Challenges encountered in using this species included susceptibility to mite infestation and low adult survival rates in the 28-day cyantraniliprole test. Environ Toxicol Chem 2024;43:1820-1835. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. |
format |
Article in Journal/Newspaper |
author |
Martin, William J Sibley, Paul K Prosser, Ryan S |
author_facet |
Martin, William J Sibley, Paul K Prosser, Ryan S |
author_sort |
Martin, William J |
title |
Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing. |
title_short |
Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing. |
title_full |
Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing. |
title_fullStr |
Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing. |
title_full_unstemmed |
Characterization of Springtail (Arrhopalites caecus) for Use in Soil Ecotoxicity Testing. |
title_sort |
characterization of springtail (arrhopalites caecus) for use in soil ecotoxicity testing. |
publisher |
Wiley |
publishDate |
2024 |
url |
https://doi.org/10.1002/etc.5898 https://pubmed.ncbi.nlm.nih.gov/38837715 |
genre |
Mite Springtail |
genre_facet |
Mite Springtail |
op_source |
Environ Toxicol Chem ISSN:1552-8618 Volume:43 Issue:8 |
op_relation |
https://doi.org/10.1002/etc.5898 https://pubmed.ncbi.nlm.nih.gov/38837715 |
op_rights |
© 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. |
op_doi |
https://doi.org/10.1002/etc.5898 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
43 |
container_issue |
8 |
container_start_page |
1820 |
op_container_end_page |
1835 |
_version_ |
1810486213116690432 |