Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infe...

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Published in:Animals
Main Authors: Šiljegović, Sara, Mouillaud, Théo, Jiolle, Davy, Petrić, Dušan, Ignjatović-Ćupina, Aleksandra, Vasić, Ana, Paupy, Christophe, Kavran, Mihaela
Format: Article in Journal/Newspaper
Language:English
Published: MDPI 2024
Subjects:
Online Access:https://doi.org/10.3390/ani14091255
https://pubmed.ncbi.nlm.nih.gov/38731259
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11083277/
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spelling ftpubmed:38731259 2024-06-09T07:50:00+00:00 Šiljegović, Sara Mouillaud, Théo Jiolle, Davy Petrić, Dušan Ignjatović-Ćupina, Aleksandra Vasić, Ana Paupy, Christophe Kavran, Mihaela 2024 Apr 23 https://doi.org/10.3390/ani14091255 https://pubmed.ncbi.nlm.nih.gov/38731259 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11083277/ eng eng MDPI https://doi.org/10.3390/ani14091255 https://pubmed.ncbi.nlm.nih.gov/38731259 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11083277/ Animals (Basel) ISSN:2076-2615 Volume:14 Issue:9 Cox1 gene Dirofilaria sp. Setaria tundra mosquito surveillance Journal Article 2024 ftpubmed https://doi.org/10.3390/ani14091255 2024-05-14T16:02:00Z Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to infect deer, has not yet been detected in Serbia but has been documented in neighboring countries. Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes of species found to be infected, in order to identify the potential source of parasite infection. A total of 2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene, 650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin (August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra. Article in Journal/Newspaper Tundra PubMed Central (PMC) Animals 14 9 1255
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Cox1 gene
Dirofilaria sp.
Setaria tundra
mosquito surveillance
spellingShingle Cox1 gene
Dirofilaria sp.
Setaria tundra
mosquito surveillance
Šiljegović, Sara
Mouillaud, Théo
Jiolle, Davy
Petrić, Dušan
Ignjatović-Ćupina, Aleksandra
Vasić, Ana
Paupy, Christophe
Kavran, Mihaela
topic_facet Cox1 gene
Dirofilaria sp.
Setaria tundra
mosquito surveillance
description Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to infect deer, has not yet been detected in Serbia but has been documented in neighboring countries. Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes of species found to be infected, in order to identify the potential source of parasite infection. A total of 2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene, 650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin (August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra.
format Article in Journal/Newspaper
author Šiljegović, Sara
Mouillaud, Théo
Jiolle, Davy
Petrić, Dušan
Ignjatović-Ćupina, Aleksandra
Vasić, Ana
Paupy, Christophe
Kavran, Mihaela
author_facet Šiljegović, Sara
Mouillaud, Théo
Jiolle, Davy
Petrić, Dušan
Ignjatović-Ćupina, Aleksandra
Vasić, Ana
Paupy, Christophe
Kavran, Mihaela
author_sort Šiljegović, Sara
publisher MDPI
publishDate 2024
url https://doi.org/10.3390/ani14091255
https://pubmed.ncbi.nlm.nih.gov/38731259
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11083277/
genre Tundra
genre_facet Tundra
op_source Animals (Basel)
ISSN:2076-2615
Volume:14
Issue:9
op_relation https://doi.org/10.3390/ani14091255
https://pubmed.ncbi.nlm.nih.gov/38731259
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11083277/
op_doi https://doi.org/10.3390/ani14091255
container_title Animals
container_volume 14
container_issue 9
container_start_page 1255
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