Microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming.
Physiological responses of soil microorganisms to global warming are important for soil ecosystem function and the terrestrial carbon cycle. Here, we investigate the effects of weeks, years, and decades of soil warming across seasons and time on the microbial protein biosynthesis machineries (i.e. r...
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Online Access: | https://doi.org/10.1093/ismejo/wrae081 https://pubmed.ncbi.nlm.nih.gov/38722823 |
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ftpubmed:38722823 2024-06-09T07:47:14+00:00 Microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming. Söllinger, Andrea Ahlers, Laureen S Dahl, Mathilde Borg Sigurðsson, Páll de Carlan, Coline Le Noir Bhattarai, Biplabi Gall, Christoph Martin, Victoria S Rottensteiner, Cornelia Motleleng, Liabo L Breines, Eva Marie Verbruggen, Erik Ostonen, Ivika Sigurdsson, Bjarni D Richter, Andreas Tveit, Alexander T 2024 May 09 https://doi.org/10.1093/ismejo/wrae081 https://pubmed.ncbi.nlm.nih.gov/38722823 eng eng Silverchair Information Systems https://doi.org/10.1093/ismejo/wrae081 https://pubmed.ncbi.nlm.nih.gov/38722823 © The Author(s) [2024]. Published by Oxford University Press on behalf of the International Society for Microbial Ecology. ISME J ISSN:1751-7370 DNA Iceland RNA forest soil grassland soil microbial physiology protein biosynthesis seasonal temperature changes soil warming temperature response Journal Article 2024 ftpubmed https://doi.org/10.1093/ismejo/wrae081 2024-05-10T16:03:00Z Physiological responses of soil microorganisms to global warming are important for soil ecosystem function and the terrestrial carbon cycle. Here, we investigate the effects of weeks, years, and decades of soil warming across seasons and time on the microbial protein biosynthesis machineries (i.e. ribosomes), the most abundant cellular macromolecular complexes, using RNA:DNA and RNA:MBC (microbial biomass carbon) ratios as proxies for cellular ribosome contents. We compared warmed soils and non-warmed controls of 15 replicated subarctic grassland and forest soil temperature gradients subject to natural geothermal warming. RNA:DNA ratios tended to be lower in the warmed soils during summer and autumn, independent of warming duration (6 weeks, 8-14 years, > 50 years), warming intensity (+3°C, +6°C, +9°C), and ecosystem type. With increasing temperatures RNA:MBC ratios were also decreasing. Additionally, seasonal RNA:DNA ratios of the consecutively sampled forest showed the same temperature-driven pattern. This suggests that subarctic soil microorganisms are depleted of ribosomes under warm conditions and the lack of consistent relationships with other physicochemical parameters besides temperature further suggests temperature as key driver. Furthermore, in incubation experiments, we measured significantly higher CO2 emission rates per unit of RNA from short- and long-term warmed soils compared to non-warmed controls. In conclusion, ribosome reduction may represent a widespread microbial physiological response to warming that offers a selective advantage at higher temperatures, as energy and matter can be reallocated from ribosome synthesis to other processes including substrate uptake and turnover. This way, ribosome reduction could have a substantial effect on soil carbon dynamics. Article in Journal/Newspaper Iceland Subarctic PubMed Central (PMC) The ISME Journal 18 1 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
DNA Iceland RNA forest soil grassland soil microbial physiology protein biosynthesis seasonal temperature changes soil warming temperature response |
spellingShingle |
DNA Iceland RNA forest soil grassland soil microbial physiology protein biosynthesis seasonal temperature changes soil warming temperature response Söllinger, Andrea Ahlers, Laureen S Dahl, Mathilde Borg Sigurðsson, Páll de Carlan, Coline Le Noir Bhattarai, Biplabi Gall, Christoph Martin, Victoria S Rottensteiner, Cornelia Motleleng, Liabo L Breines, Eva Marie Verbruggen, Erik Ostonen, Ivika Sigurdsson, Bjarni D Richter, Andreas Tveit, Alexander T Microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming. |
topic_facet |
DNA Iceland RNA forest soil grassland soil microbial physiology protein biosynthesis seasonal temperature changes soil warming temperature response |
description |
Physiological responses of soil microorganisms to global warming are important for soil ecosystem function and the terrestrial carbon cycle. Here, we investigate the effects of weeks, years, and decades of soil warming across seasons and time on the microbial protein biosynthesis machineries (i.e. ribosomes), the most abundant cellular macromolecular complexes, using RNA:DNA and RNA:MBC (microbial biomass carbon) ratios as proxies for cellular ribosome contents. We compared warmed soils and non-warmed controls of 15 replicated subarctic grassland and forest soil temperature gradients subject to natural geothermal warming. RNA:DNA ratios tended to be lower in the warmed soils during summer and autumn, independent of warming duration (6 weeks, 8-14 years, > 50 years), warming intensity (+3°C, +6°C, +9°C), and ecosystem type. With increasing temperatures RNA:MBC ratios were also decreasing. Additionally, seasonal RNA:DNA ratios of the consecutively sampled forest showed the same temperature-driven pattern. This suggests that subarctic soil microorganisms are depleted of ribosomes under warm conditions and the lack of consistent relationships with other physicochemical parameters besides temperature further suggests temperature as key driver. Furthermore, in incubation experiments, we measured significantly higher CO2 emission rates per unit of RNA from short- and long-term warmed soils compared to non-warmed controls. In conclusion, ribosome reduction may represent a widespread microbial physiological response to warming that offers a selective advantage at higher temperatures, as energy and matter can be reallocated from ribosome synthesis to other processes including substrate uptake and turnover. This way, ribosome reduction could have a substantial effect on soil carbon dynamics. |
format |
Article in Journal/Newspaper |
author |
Söllinger, Andrea Ahlers, Laureen S Dahl, Mathilde Borg Sigurðsson, Páll de Carlan, Coline Le Noir Bhattarai, Biplabi Gall, Christoph Martin, Victoria S Rottensteiner, Cornelia Motleleng, Liabo L Breines, Eva Marie Verbruggen, Erik Ostonen, Ivika Sigurdsson, Bjarni D Richter, Andreas Tveit, Alexander T |
author_facet |
Söllinger, Andrea Ahlers, Laureen S Dahl, Mathilde Borg Sigurðsson, Páll de Carlan, Coline Le Noir Bhattarai, Biplabi Gall, Christoph Martin, Victoria S Rottensteiner, Cornelia Motleleng, Liabo L Breines, Eva Marie Verbruggen, Erik Ostonen, Ivika Sigurdsson, Bjarni D Richter, Andreas Tveit, Alexander T |
author_sort |
Söllinger, Andrea |
title |
Microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming. |
title_short |
Microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming. |
title_full |
Microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming. |
title_fullStr |
Microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming. |
title_full_unstemmed |
Microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming. |
title_sort |
microorganisms in subarctic soils are depleted of ribosomes under short-, medium-, and long-term warming. |
publisher |
Silverchair Information Systems |
publishDate |
2024 |
url |
https://doi.org/10.1093/ismejo/wrae081 https://pubmed.ncbi.nlm.nih.gov/38722823 |
genre |
Iceland Subarctic |
genre_facet |
Iceland Subarctic |
op_source |
ISME J ISSN:1751-7370 |
op_relation |
https://doi.org/10.1093/ismejo/wrae081 https://pubmed.ncbi.nlm.nih.gov/38722823 |
op_rights |
© The Author(s) [2024]. Published by Oxford University Press on behalf of the International Society for Microbial Ecology. |
op_doi |
https://doi.org/10.1093/ismejo/wrae081 |
container_title |
The ISME Journal |
container_volume |
18 |
container_issue |
1 |
_version_ |
1801378189737459712 |