A yellow pigmented, Gram-stain-positive, motile, facultatively anaerobic and irregular rod-shaped bacteria (strain M0-14T) was isolated from a till sample collected from the foreland of a high Arctic glacier near the settlement of Ny-Ålesund in the Svalbard Archipelago, Norway. Phylogenetic analysis...
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Online Access: | https://doi.org/10.1099/ijsem.0.006368 https://pubmed.ncbi.nlm.nih.gov/38722773 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165906/ |
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ftpubmed:38722773 2024-06-23T07:50:16+00:00 Xie, Jialin Ren, Lvzhi Wei, Ziyan Peng, Xiaoya Qin, Kun Peng, Fang 2024 May https://doi.org/10.1099/ijsem.0.006368 https://pubmed.ncbi.nlm.nih.gov/38722773 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165906/ eng eng Ingenta plc https://doi.org/10.1099/ijsem.0.006368 https://pubmed.ncbi.nlm.nih.gov/38722773 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165906/ Int J Syst Evol Microbiol ISSN:1466-5034 Volume:74 Issue:5 Pengzhenrongella phosphoraccumulans Svalbard Archipelago high-Arctic glacial till Journal Article 2024 ftpubmed https://doi.org/10.1099/ijsem.0.006368 2024-06-13T16:02:00Z A yellow pigmented, Gram-stain-positive, motile, facultatively anaerobic and irregular rod-shaped bacteria (strain M0-14T) was isolated from a till sample collected from the foreland of a high Arctic glacier near the settlement of Ny-Ålesund in the Svalbard Archipelago, Norway. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that M0-14T formed a lineage within the family Cellulomonadaceae, suborder Micrococcineae. M0-14T represented a novel member of the genus Pengzhenrongella and had highest 16S rRNA gene sequence similarity to Pengzhenrongella sicca LRZ-2T (97.3 %). Growth occurred at 4-25 °C (optimum 4-18 °C), at pH 6.0-9.0 (optimum pH 7.0), and in the presence of 0-5 % (w/v) NaCl. The predominant menaquinone was MK-9(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major polar lipids were phosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylinositol, one undefined phospholipid and five undefined phosphoglycolipids. The cell-wall diamino acid was l-ornithine whereas rhamnose and mannose were the cell-wall sugars. Polyphosphate particles were found inside the cells of M0-14T. Polyphosphate kinase and polyphosphate-dependent glucokinase genes were detected during genomic sequencing of M0-14. In addition, the complete pstSCAB gene cluster and phnCDE synthesis genes, which are important for the uptake and transport of phosphorus in cells, were annotated in the genomic data. According to the genomic data, M0-14T has a metabolic pathway related to phosphorus accumulation. The DNA G+C content of the genomic DNA was 70.8 %. On the basis of its phylogenetic relationship, phenotypic properties and chemotaxonomic distinctiveness, strain M0-14T represents a novel species of the genus Pengzhenrongella, for which the name Pengzhenrongella phosphoraccumulans sp. nov. is proposed. The type strain is M0-14T (= CCTCC AB 2012967T = NRRL B-59105T). Article in Journal/Newspaper Arctic glacier glacier Ny Ålesund Ny-Ålesund Svalbard PubMed Central (PMC) Arctic Norway Ny-Ålesund Svalbard Svalbard Archipelago International Journal of Systematic and Evolutionary Microbiology 74 5 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Pengzhenrongella phosphoraccumulans Svalbard Archipelago high-Arctic glacial till |
spellingShingle |
Pengzhenrongella phosphoraccumulans Svalbard Archipelago high-Arctic glacial till Xie, Jialin Ren, Lvzhi Wei, Ziyan Peng, Xiaoya Qin, Kun Peng, Fang |
topic_facet |
Pengzhenrongella phosphoraccumulans Svalbard Archipelago high-Arctic glacial till |
description |
A yellow pigmented, Gram-stain-positive, motile, facultatively anaerobic and irregular rod-shaped bacteria (strain M0-14T) was isolated from a till sample collected from the foreland of a high Arctic glacier near the settlement of Ny-Ålesund in the Svalbard Archipelago, Norway. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that M0-14T formed a lineage within the family Cellulomonadaceae, suborder Micrococcineae. M0-14T represented a novel member of the genus Pengzhenrongella and had highest 16S rRNA gene sequence similarity to Pengzhenrongella sicca LRZ-2T (97.3 %). Growth occurred at 4-25 °C (optimum 4-18 °C), at pH 6.0-9.0 (optimum pH 7.0), and in the presence of 0-5 % (w/v) NaCl. The predominant menaquinone was MK-9(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major polar lipids were phosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylinositol, one undefined phospholipid and five undefined phosphoglycolipids. The cell-wall diamino acid was l-ornithine whereas rhamnose and mannose were the cell-wall sugars. Polyphosphate particles were found inside the cells of M0-14T. Polyphosphate kinase and polyphosphate-dependent glucokinase genes were detected during genomic sequencing of M0-14. In addition, the complete pstSCAB gene cluster and phnCDE synthesis genes, which are important for the uptake and transport of phosphorus in cells, were annotated in the genomic data. According to the genomic data, M0-14T has a metabolic pathway related to phosphorus accumulation. The DNA G+C content of the genomic DNA was 70.8 %. On the basis of its phylogenetic relationship, phenotypic properties and chemotaxonomic distinctiveness, strain M0-14T represents a novel species of the genus Pengzhenrongella, for which the name Pengzhenrongella phosphoraccumulans sp. nov. is proposed. The type strain is M0-14T (= CCTCC AB 2012967T = NRRL B-59105T). |
format |
Article in Journal/Newspaper |
author |
Xie, Jialin Ren, Lvzhi Wei, Ziyan Peng, Xiaoya Qin, Kun Peng, Fang |
author_facet |
Xie, Jialin Ren, Lvzhi Wei, Ziyan Peng, Xiaoya Qin, Kun Peng, Fang |
author_sort |
Xie, Jialin |
publisher |
Ingenta plc |
publishDate |
2024 |
url |
https://doi.org/10.1099/ijsem.0.006368 https://pubmed.ncbi.nlm.nih.gov/38722773 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165906/ |
geographic |
Arctic Norway Ny-Ålesund Svalbard Svalbard Archipelago |
geographic_facet |
Arctic Norway Ny-Ålesund Svalbard Svalbard Archipelago |
genre |
Arctic glacier glacier Ny Ålesund Ny-Ålesund Svalbard |
genre_facet |
Arctic glacier glacier Ny Ålesund Ny-Ålesund Svalbard |
op_source |
Int J Syst Evol Microbiol ISSN:1466-5034 Volume:74 Issue:5 |
op_relation |
https://doi.org/10.1099/ijsem.0.006368 https://pubmed.ncbi.nlm.nih.gov/38722773 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11165906/ |
op_doi |
https://doi.org/10.1099/ijsem.0.006368 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
74 |
container_issue |
5 |
_version_ |
1802641136028745728 |