Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota.
Actinomycetota have been widely described as valuable sources for the acquisition of secondary metabolites. Most microbial metabolites are produced via metabolic pathways encoded by biosynthetic gene clusters (BGCs). Although many secondary metabolites are not essential for the survival of bacteria,...
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ftpubmed:38717668 2024-06-09T07:41:09+00:00 Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota. Bruna, Pablo Núñez-Montero, Kattia Contreras, María José Leal, Karla García, Matías Abanto, Michel Barrientos, Leticia 2024 May 08 https://doi.org/10.1007/s00253-024-13154-x https://pubmed.ncbi.nlm.nih.gov/38717668 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078813/ eng eng Springer https://doi.org/10.1007/s00253-024-13154-x https://pubmed.ncbi.nlm.nih.gov/38717668 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078813/ © 2024. The Author(s). Appl Microbiol Biotechnol ISSN:1432-0614 Volume:108 Issue:1 Micrococcaceae Actinomycetota Antarctic Biosynthetic gene clusters Secondary metabolites Journal Article 2024 ftpubmed https://doi.org/10.1007/s00253-024-13154-x 2024-05-11T16:02:00Z Actinomycetota have been widely described as valuable sources for the acquisition of secondary metabolites. Most microbial metabolites are produced via metabolic pathways encoded by biosynthetic gene clusters (BGCs). Although many secondary metabolites are not essential for the survival of bacteria, they play an important role in their adaptation and interactions within microbial communities. This is how bacteria isolated from extreme environments such as Antarctica could facilitate the discovery of new BGCs with biotechnological potential. This study aimed to isolate rare Actinomycetota strains from Antarctic soil and sediment samples and identify their metabolic potential based on genome mining and exploration of biosynthetic gene clusters. To this end, the strains were sequenced using Illumina and Oxford Nanopore Technologies platforms. The assemblies were annotated and subjected to phylogenetic analysis. Finally, the BGCs present in each genome were identified using the antiSMASH tool, and the biosynthetic diversity of the Micrococcaceae family was evaluated. Taxonomic annotation revealed that seven strains were new and two were previously reported in the NCBI database. Additionally, BGCs encoding type III polyketide synthases (T3PKS), beta-lactones, siderophores, and non-ribosomal peptide synthetases (NRPS) have been identified, among others. In addition, the sequence similarity network showed a predominant type of BGCs in the family Micrococcaceae, and some genera were distinctly grouped. The BGCs identified in the isolated strains could be associated with applications such as antimicrobials, anticancer agents, and plant growth promoters, among others, positioning them as excellent candidates for future biotechnological applications and innovations. KEY POINTS: • Novel Antarctic rare Actinomycetota strains were isolated from soil and sediments • Genome-based taxonomic affiliation revealed seven potentially novel species • Genome mining showed metabolic potential for novel natural products. Article in Journal/Newspaper Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic Applied Microbiology and Biotechnology 108 1 |
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Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Micrococcaceae Actinomycetota Antarctic Biosynthetic gene clusters Secondary metabolites |
spellingShingle |
Micrococcaceae Actinomycetota Antarctic Biosynthetic gene clusters Secondary metabolites Bruna, Pablo Núñez-Montero, Kattia Contreras, María José Leal, Karla García, Matías Abanto, Michel Barrientos, Leticia Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota. |
topic_facet |
Micrococcaceae Actinomycetota Antarctic Biosynthetic gene clusters Secondary metabolites |
description |
Actinomycetota have been widely described as valuable sources for the acquisition of secondary metabolites. Most microbial metabolites are produced via metabolic pathways encoded by biosynthetic gene clusters (BGCs). Although many secondary metabolites are not essential for the survival of bacteria, they play an important role in their adaptation and interactions within microbial communities. This is how bacteria isolated from extreme environments such as Antarctica could facilitate the discovery of new BGCs with biotechnological potential. This study aimed to isolate rare Actinomycetota strains from Antarctic soil and sediment samples and identify their metabolic potential based on genome mining and exploration of biosynthetic gene clusters. To this end, the strains were sequenced using Illumina and Oxford Nanopore Technologies platforms. The assemblies were annotated and subjected to phylogenetic analysis. Finally, the BGCs present in each genome were identified using the antiSMASH tool, and the biosynthetic diversity of the Micrococcaceae family was evaluated. Taxonomic annotation revealed that seven strains were new and two were previously reported in the NCBI database. Additionally, BGCs encoding type III polyketide synthases (T3PKS), beta-lactones, siderophores, and non-ribosomal peptide synthetases (NRPS) have been identified, among others. In addition, the sequence similarity network showed a predominant type of BGCs in the family Micrococcaceae, and some genera were distinctly grouped. The BGCs identified in the isolated strains could be associated with applications such as antimicrobials, anticancer agents, and plant growth promoters, among others, positioning them as excellent candidates for future biotechnological applications and innovations. KEY POINTS: • Novel Antarctic rare Actinomycetota strains were isolated from soil and sediments • Genome-based taxonomic affiliation revealed seven potentially novel species • Genome mining showed metabolic potential for novel natural products. |
format |
Article in Journal/Newspaper |
author |
Bruna, Pablo Núñez-Montero, Kattia Contreras, María José Leal, Karla García, Matías Abanto, Michel Barrientos, Leticia |
author_facet |
Bruna, Pablo Núñez-Montero, Kattia Contreras, María José Leal, Karla García, Matías Abanto, Michel Barrientos, Leticia |
author_sort |
Bruna, Pablo |
title |
Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota. |
title_short |
Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota. |
title_full |
Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota. |
title_fullStr |
Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota. |
title_full_unstemmed |
Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota. |
title_sort |
biosynthetic gene clusters with biotechnological applications in novel antarctic isolates from actinomycetota. |
publisher |
Springer |
publishDate |
2024 |
url |
https://doi.org/10.1007/s00253-024-13154-x https://pubmed.ncbi.nlm.nih.gov/38717668 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078813/ |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Appl Microbiol Biotechnol ISSN:1432-0614 Volume:108 Issue:1 |
op_relation |
https://doi.org/10.1007/s00253-024-13154-x https://pubmed.ncbi.nlm.nih.gov/38717668 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078813/ |
op_rights |
© 2024. The Author(s). |
op_doi |
https://doi.org/10.1007/s00253-024-13154-x |
container_title |
Applied Microbiology and Biotechnology |
container_volume |
108 |
container_issue |
1 |
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1801369595125170176 |