New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves.
The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound influenc...
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ftpubmed:38666797 2024-09-15T17:47:03+00:00 New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves. Llorenç-Vicedo, Aitana Lluesma Gomez, Monica Zeising, Ole Kleiner, Thomas Freitag, Johannes Martinez-Hernandez, Francisco Wilhelms, Frank Martinez-Garcia, Manuel 2024 May 29 https://doi.org/10.1128/msphere.00073-24 https://pubmed.ncbi.nlm.nih.gov/38666797 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237435/ eng eng Atypon https://doi.org/10.1128/msphere.00073-24 https://pubmed.ncbi.nlm.nih.gov/38666797 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237435/ mSphere ISSN:2379-5042 Volume:9 Issue:5 Antarctic DNA sequencing bacteria ice shelf marine ice metagenomics microbiome single-cell genomics Journal Article 2024 ftpubmed https://doi.org/10.1128/msphere.00073-24 2024-07-14T16:03:00Z The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound influence on global carbon cycles. Beneath Antarctic ice shelves, marine ice stores valuable genetic information, where marine microbial communities before the industrial revolution are archived. Here, in this proof-of-concept, by employing a combination of single-cell technologiesand metagenomics, we have been able to sequence frozen microbial DNA (≈300 years old) stored in the marine ice core B15 collected from the Filchnner-Ronne Ice Shelf. Metagenomic data indicated that Proteobacteria and Thaumarchaeota (e.g., Nitrosopumilus spp.), followed by Actinobacteria (e.g., Actinomarinales), were abundant. Remarkably, our data allow us to "travel to the past" and calibrate genomic and genetic evolutionary changes for ecologically relevant microbes and functions, such as Nitrosopumilus spp., preserved in the marine ice (≈300 years old) with those collected recently in seawater under an ice shelf (year 2017). The evolutionary divergence for the ammonia monooxygenase gene amoA involved in chemolithoautotrophy was about 0.88 amino acid and 2.8 nucleotide substitution rate per 100 sites in a century, while the accumulated rate of genomic SNPs was 2,467 per 1 Mb of genome and 100 years. Whether these evolutionary changes remained constant over the last 300 years or accelerated during post-industrial periods remains an open question that will be further elucidated. Article in Journal/Newspaper Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf PubMed Central (PMC) mSphere 9 5 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Antarctic DNA sequencing bacteria ice shelf marine ice metagenomics microbiome single-cell genomics |
spellingShingle |
Antarctic DNA sequencing bacteria ice shelf marine ice metagenomics microbiome single-cell genomics Llorenç-Vicedo, Aitana Lluesma Gomez, Monica Zeising, Ole Kleiner, Thomas Freitag, Johannes Martinez-Hernandez, Francisco Wilhelms, Frank Martinez-Garcia, Manuel New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves. |
topic_facet |
Antarctic DNA sequencing bacteria ice shelf marine ice metagenomics microbiome single-cell genomics |
description |
The signs of climate change are undeniable, and the impact of these changes on ecosystem function heavily depends on the response of microbes that underpin the food web. Antarctic ice shelf is a massive mass of floating ice that extends from the continent into the ocean, exerting a profound influence on global carbon cycles. Beneath Antarctic ice shelves, marine ice stores valuable genetic information, where marine microbial communities before the industrial revolution are archived. Here, in this proof-of-concept, by employing a combination of single-cell technologiesand metagenomics, we have been able to sequence frozen microbial DNA (≈300 years old) stored in the marine ice core B15 collected from the Filchnner-Ronne Ice Shelf. Metagenomic data indicated that Proteobacteria and Thaumarchaeota (e.g., Nitrosopumilus spp.), followed by Actinobacteria (e.g., Actinomarinales), were abundant. Remarkably, our data allow us to "travel to the past" and calibrate genomic and genetic evolutionary changes for ecologically relevant microbes and functions, such as Nitrosopumilus spp., preserved in the marine ice (≈300 years old) with those collected recently in seawater under an ice shelf (year 2017). The evolutionary divergence for the ammonia monooxygenase gene amoA involved in chemolithoautotrophy was about 0.88 amino acid and 2.8 nucleotide substitution rate per 100 sites in a century, while the accumulated rate of genomic SNPs was 2,467 per 1 Mb of genome and 100 years. Whether these evolutionary changes remained constant over the last 300 years or accelerated during post-industrial periods remains an open question that will be further elucidated. |
format |
Article in Journal/Newspaper |
author |
Llorenç-Vicedo, Aitana Lluesma Gomez, Monica Zeising, Ole Kleiner, Thomas Freitag, Johannes Martinez-Hernandez, Francisco Wilhelms, Frank Martinez-Garcia, Manuel |
author_facet |
Llorenç-Vicedo, Aitana Lluesma Gomez, Monica Zeising, Ole Kleiner, Thomas Freitag, Johannes Martinez-Hernandez, Francisco Wilhelms, Frank Martinez-Garcia, Manuel |
author_sort |
Llorenç-Vicedo, Aitana |
title |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves. |
title_short |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves. |
title_full |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves. |
title_fullStr |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves. |
title_full_unstemmed |
New avenues for potentially seeking microbial responses to climate change beneath Antarctic ice shelves. |
title_sort |
new avenues for potentially seeking microbial responses to climate change beneath antarctic ice shelves. |
publisher |
Atypon |
publishDate |
2024 |
url |
https://doi.org/10.1128/msphere.00073-24 https://pubmed.ncbi.nlm.nih.gov/38666797 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237435/ |
genre |
Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf |
genre_facet |
Antarc* Antarctic ice core Ice Shelf Ice Shelves Ronne Ice Shelf |
op_source |
mSphere ISSN:2379-5042 Volume:9 Issue:5 |
op_relation |
https://doi.org/10.1128/msphere.00073-24 https://pubmed.ncbi.nlm.nih.gov/38666797 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237435/ |
op_doi |
https://doi.org/10.1128/msphere.00073-24 |
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mSphere |
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9 |
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5 |
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1810495583030345728 |