Exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of Crassostrea gigas.
The Notch signaling pathway plays a pivotal role in governing cell fate determinations within the gonadal niche. This study provides an extensive elucidation of the male and female gonadal niches within Crassostrea gigas. Examination via transmission electron microscopy revealed the presence of desm...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Online Access: | https://doi.org/10.1016/j.cbpa.2024.111639 https://pubmed.ncbi.nlm.nih.gov/38641164 |
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ftpubmed:38641164 2024-09-15T18:03:04+00:00 Exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of Crassostrea gigas. Wang, Huihui Yu, Hong Li, Qi 2024-08 https://doi.org/10.1016/j.cbpa.2024.111639 https://pubmed.ncbi.nlm.nih.gov/38641164 eng eng Elsevier Science https://doi.org/10.1016/j.cbpa.2024.111639 https://pubmed.ncbi.nlm.nih.gov/38641164 Copyright © 2024 Elsevier Inc. All rights reserved. Comp Biochem Physiol A Mol Integr Physiol ISSN:1531-4332 Volume:294 Crassostrea gigas Desmosome-like Gonadal niche Notch signaling pathway Journal Article Research Support, Non-U.S. Gov't 2024 ftpubmed https://doi.org/10.1016/j.cbpa.2024.111639 2024-07-04T16:01:00Z The Notch signaling pathway plays a pivotal role in governing cell fate determinations within the gonadal niche. This study provides an extensive elucidation of the male and female gonadal niches within Crassostrea gigas. Examination via transmission electron microscopy revealed the presence of desmosome-like connection not only between germ cells and niche cells but also among adjacent niche cells within the oyster gonad. Transcriptomic analysis identified several putative Notch pathway components, including CgJAG1, CgNOTCH1, CgSuh, and CgHey1. Phylogenetic analysis indicated a close evolutionary relationship between CgJAG1, CgNOTCH1, and CgHey1 and Notch members present in Drosophila. Expression profiling results indicated a notable abundance of CgHey1 in the gonads, while CgJAG1 and CgNOTCH1 displayed distinct expression patterns associated with sexual dimorphism. In situ hybridization findings corroborated the predominant expression of CgJAG1 in male niche cells, while CgNOTCH1 was expressed in both male and female germ cells, as well as female niche cells. These findings demonstrate the important role of the Notch signaling pathway in the gonadal niche of oysters. Article in Journal/Newspaper Crassostrea gigas PubMed Central (PMC) Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 294 111639 |
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PubMed Central (PMC) |
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language |
English |
topic |
Crassostrea gigas Desmosome-like Gonadal niche Notch signaling pathway |
spellingShingle |
Crassostrea gigas Desmosome-like Gonadal niche Notch signaling pathway Wang, Huihui Yu, Hong Li, Qi Exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of Crassostrea gigas. |
topic_facet |
Crassostrea gigas Desmosome-like Gonadal niche Notch signaling pathway |
description |
The Notch signaling pathway plays a pivotal role in governing cell fate determinations within the gonadal niche. This study provides an extensive elucidation of the male and female gonadal niches within Crassostrea gigas. Examination via transmission electron microscopy revealed the presence of desmosome-like connection not only between germ cells and niche cells but also among adjacent niche cells within the oyster gonad. Transcriptomic analysis identified several putative Notch pathway components, including CgJAG1, CgNOTCH1, CgSuh, and CgHey1. Phylogenetic analysis indicated a close evolutionary relationship between CgJAG1, CgNOTCH1, and CgHey1 and Notch members present in Drosophila. Expression profiling results indicated a notable abundance of CgHey1 in the gonads, while CgJAG1 and CgNOTCH1 displayed distinct expression patterns associated with sexual dimorphism. In situ hybridization findings corroborated the predominant expression of CgJAG1 in male niche cells, while CgNOTCH1 was expressed in both male and female germ cells, as well as female niche cells. These findings demonstrate the important role of the Notch signaling pathway in the gonadal niche of oysters. |
format |
Article in Journal/Newspaper |
author |
Wang, Huihui Yu, Hong Li, Qi |
author_facet |
Wang, Huihui Yu, Hong Li, Qi |
author_sort |
Wang, Huihui |
title |
Exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of Crassostrea gigas. |
title_short |
Exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of Crassostrea gigas. |
title_full |
Exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of Crassostrea gigas. |
title_fullStr |
Exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of Crassostrea gigas. |
title_full_unstemmed |
Exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of Crassostrea gigas. |
title_sort |
exploration of cell-cell interactions and the notch signaling pathway in the gonadal niche of crassostrea gigas. |
publisher |
Elsevier Science |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.cbpa.2024.111639 https://pubmed.ncbi.nlm.nih.gov/38641164 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Comp Biochem Physiol A Mol Integr Physiol ISSN:1531-4332 Volume:294 |
op_relation |
https://doi.org/10.1016/j.cbpa.2024.111639 https://pubmed.ncbi.nlm.nih.gov/38641164 |
op_rights |
Copyright © 2024 Elsevier Inc. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.cbpa.2024.111639 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
294 |
container_start_page |
111639 |
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1810440589701808128 |