A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas.
Trace amine-associated receptors (TAARs) are a class of G protein-coupled receptors, playing an immunomodulatory function in the neuroinflammatory responses. In the present study, a TAAR homologue with a 7tm_classA_rhodopsin-like domain (designated as CgTAAR1L) was identified in oyster Crassostrea g...
Published in: | Developmental & Comparative Immunology |
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ftpubmed:38537729 2024-06-09T07:45:30+00:00 A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas. Yang, Yuehong Qiao, Xue Yu, Simiao Zhao, Xinyu Jin, Yuhao Liu, Rui Li, Jie Wang, Lingling Song, Linsheng 2024 Jul https://doi.org/10.1016/j.dci.2024.105171 https://pubmed.ncbi.nlm.nih.gov/38537729 eng eng Elsevier Science https://doi.org/10.1016/j.dci.2024.105171 https://pubmed.ncbi.nlm.nih.gov/38537729 Copyright © 2024 Elsevier Ltd. All rights reserved. Dev Comp Immunol ISSN:1879-0089 Volume:156 Antimicrobial immune response Crassostrea gigas Defensin Interleukin TAAR1L Journal Article Research Support, Non-U.S. Gov't 2024 ftpubmed https://doi.org/10.1016/j.dci.2024.105171 2024-05-14T16:02:00Z Trace amine-associated receptors (TAARs) are a class of G protein-coupled receptors, playing an immunomodulatory function in the neuroinflammatory responses. In the present study, a TAAR homologue with a 7tm_classA_rhodopsin-like domain (designated as CgTAAR1L) was identified in oyster Crassostrea gigas. The abundant CgTAAR1L transcripts were detected in visceral ganglia and haemocytes compared to other tissues, which were 55.35-fold and 32.95-fold (p < 0.01) of those in adductor muscle, respectively. The mRNA expression level of CgTAAR1L in haemocytes significantly increased and reached the peak level at 3 h after LPS or Poly (I:C) stimulation, which was 4.55-fold and 12.35-fold of that in control group, respectively (p < 0.01). After the expression of CgTAAR1L was inhibited by the injection of its targeted siRNA, the mRNA expression levels of interleukin17s (CgIL17-1, CgIL17-5 and CgIL17-6), and defensin (Cgdefh1) significantly decreased at 3 h after LPS stimulation, which was 0.51-fold (p < 0.001), 0.39-fold (p < 0.01), 0.48-fold (p < 0.05) and 0.41-fold (p < 0.05) of that in the control group, respectively. The nuclear translocation of Cgp65 protein was suppressed in the CgTAAR1L-RNAi oysters. Furthermore, the number of Vibrio splendidus in the haemolymph of CgTAAR1L-RNAi oysters significantly increased (4.11-fold, p < 0.001) compared with that in the control group. In contrast, there was no significant difference in phagocytic rate of haemocytes to V. splendidus in the CgTAAR1L-RNAi oysters. These results indicated that CgTAAR1L played an important role in the immune defense against bacterial infection by inducing the expressions of interleukin and defensin. Article in Journal/Newspaper Crassostrea gigas PubMed Central (PMC) Developmental & Comparative Immunology 156 105171 |
institution |
Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Antimicrobial immune response Crassostrea gigas Defensin Interleukin TAAR1L |
spellingShingle |
Antimicrobial immune response Crassostrea gigas Defensin Interleukin TAAR1L Yang, Yuehong Qiao, Xue Yu, Simiao Zhao, Xinyu Jin, Yuhao Liu, Rui Li, Jie Wang, Lingling Song, Linsheng A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas. |
topic_facet |
Antimicrobial immune response Crassostrea gigas Defensin Interleukin TAAR1L |
description |
Trace amine-associated receptors (TAARs) are a class of G protein-coupled receptors, playing an immunomodulatory function in the neuroinflammatory responses. In the present study, a TAAR homologue with a 7tm_classA_rhodopsin-like domain (designated as CgTAAR1L) was identified in oyster Crassostrea gigas. The abundant CgTAAR1L transcripts were detected in visceral ganglia and haemocytes compared to other tissues, which were 55.35-fold and 32.95-fold (p < 0.01) of those in adductor muscle, respectively. The mRNA expression level of CgTAAR1L in haemocytes significantly increased and reached the peak level at 3 h after LPS or Poly (I:C) stimulation, which was 4.55-fold and 12.35-fold of that in control group, respectively (p < 0.01). After the expression of CgTAAR1L was inhibited by the injection of its targeted siRNA, the mRNA expression levels of interleukin17s (CgIL17-1, CgIL17-5 and CgIL17-6), and defensin (Cgdefh1) significantly decreased at 3 h after LPS stimulation, which was 0.51-fold (p < 0.001), 0.39-fold (p < 0.01), 0.48-fold (p < 0.05) and 0.41-fold (p < 0.05) of that in the control group, respectively. The nuclear translocation of Cgp65 protein was suppressed in the CgTAAR1L-RNAi oysters. Furthermore, the number of Vibrio splendidus in the haemolymph of CgTAAR1L-RNAi oysters significantly increased (4.11-fold, p < 0.001) compared with that in the control group. In contrast, there was no significant difference in phagocytic rate of haemocytes to V. splendidus in the CgTAAR1L-RNAi oysters. These results indicated that CgTAAR1L played an important role in the immune defense against bacterial infection by inducing the expressions of interleukin and defensin. |
format |
Article in Journal/Newspaper |
author |
Yang, Yuehong Qiao, Xue Yu, Simiao Zhao, Xinyu Jin, Yuhao Liu, Rui Li, Jie Wang, Lingling Song, Linsheng |
author_facet |
Yang, Yuehong Qiao, Xue Yu, Simiao Zhao, Xinyu Jin, Yuhao Liu, Rui Li, Jie Wang, Lingling Song, Linsheng |
author_sort |
Yang, Yuehong |
title |
A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas. |
title_short |
A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas. |
title_full |
A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas. |
title_fullStr |
A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas. |
title_full_unstemmed |
A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas. |
title_sort |
trace amine associated receptor mediates antimicrobial immune response in the oyster crassostrea gigas. |
publisher |
Elsevier Science |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.dci.2024.105171 https://pubmed.ncbi.nlm.nih.gov/38537729 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Dev Comp Immunol ISSN:1879-0089 Volume:156 |
op_relation |
https://doi.org/10.1016/j.dci.2024.105171 https://pubmed.ncbi.nlm.nih.gov/38537729 |
op_rights |
Copyright © 2024 Elsevier Ltd. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.dci.2024.105171 |
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Developmental & Comparative Immunology |
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156 |
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105171 |
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1801374887519977472 |