Transcriptomic and Physiological Analysis Reveal Melanin Synthesis-Related Genes and Pathways in Pacific Oysters (Crassostrea gigas).
Shell color is one of the shell traits of molluscs, which has been regarded as an economic trait in some bivalves. Pacific oysters (Crassostrea gigas) are important aquaculture shellfish worldwide. In the past decade, several shell color strains of C. gigas were developed through selective breeding,...
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ftpubmed:38483671 2024-06-02T08:05:35+00:00 Transcriptomic and Physiological Analysis Reveal Melanin Synthesis-Related Genes and Pathways in Pacific Oysters (Crassostrea gigas). Jiang, Kunyin Xu, Chengxun Yu, Hong Kong, Lingfeng Liu, Shikai Li, Qi 2024 Apr https://doi.org/10.1007/s10126-024-10302-2 https://pubmed.ncbi.nlm.nih.gov/38483671 eng eng Springer https://doi.org/10.1007/s10126-024-10302-2 https://pubmed.ncbi.nlm.nih.gov/38483671 © 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. Mar Biotechnol (NY) ISSN:1436-2236 Volume:26 Issue:2 Crassostrea gigas Mantle cell culture Melanin synthesis Shell color Transcriptomic and physiological analysis Journal Article 2024 ftpubmed https://doi.org/10.1007/s10126-024-10302-2 2024-05-07T16:02:00Z Shell color is one of the shell traits of molluscs, which has been regarded as an economic trait in some bivalves. Pacific oysters (Crassostrea gigas) are important aquaculture shellfish worldwide. In the past decade, several shell color strains of C. gigas were developed through selective breeding, which provides valuable materials for research on the inheritance pattern and regulation mechanisms of shell color. The inheritance patterns of different shell colors in C. gigas have been identified in certain research; however, the regulation mechanism of oyster pigmentation and shell color formation remains unclear. In this study, we performed transcriptomic and physiological analyses using black and white shell oysters to investigate the molecular mechanism of melanin synthesis in C. gigas. Several pigmentation-related pathways, such as cytochrome P450, melanogenesis, tyrosine metabolism, and the cAMP signaling pathway were found. The majority of differentially expressed genes and some signaling molecules from these pathways exhibited a higher level in the black shell oysters than in the white, especially after L-tyrosine feeding, suggesting that those differences may cause a variation of tyrosine metabolism and melanin synthesis. In addition, the in vitro assay using primary cells from mantle tissue showed that L-tyrosine incubation increased cAMP level, gene and protein expression, and melanin content. This study reveals the difference in tyrosine metabolism and melanin synthesis in black and white shell oysters and provides evidence for the potential regulatory mechanism of shell color in oysters. Article in Journal/Newspaper Crassostrea gigas PubMed Central (PMC) Pacific Marine Biotechnology 26 2 364 379 |
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language |
English |
topic |
Crassostrea gigas Mantle cell culture Melanin synthesis Shell color Transcriptomic and physiological analysis |
spellingShingle |
Crassostrea gigas Mantle cell culture Melanin synthesis Shell color Transcriptomic and physiological analysis Jiang, Kunyin Xu, Chengxun Yu, Hong Kong, Lingfeng Liu, Shikai Li, Qi Transcriptomic and Physiological Analysis Reveal Melanin Synthesis-Related Genes and Pathways in Pacific Oysters (Crassostrea gigas). |
topic_facet |
Crassostrea gigas Mantle cell culture Melanin synthesis Shell color Transcriptomic and physiological analysis |
description |
Shell color is one of the shell traits of molluscs, which has been regarded as an economic trait in some bivalves. Pacific oysters (Crassostrea gigas) are important aquaculture shellfish worldwide. In the past decade, several shell color strains of C. gigas were developed through selective breeding, which provides valuable materials for research on the inheritance pattern and regulation mechanisms of shell color. The inheritance patterns of different shell colors in C. gigas have been identified in certain research; however, the regulation mechanism of oyster pigmentation and shell color formation remains unclear. In this study, we performed transcriptomic and physiological analyses using black and white shell oysters to investigate the molecular mechanism of melanin synthesis in C. gigas. Several pigmentation-related pathways, such as cytochrome P450, melanogenesis, tyrosine metabolism, and the cAMP signaling pathway were found. The majority of differentially expressed genes and some signaling molecules from these pathways exhibited a higher level in the black shell oysters than in the white, especially after L-tyrosine feeding, suggesting that those differences may cause a variation of tyrosine metabolism and melanin synthesis. In addition, the in vitro assay using primary cells from mantle tissue showed that L-tyrosine incubation increased cAMP level, gene and protein expression, and melanin content. This study reveals the difference in tyrosine metabolism and melanin synthesis in black and white shell oysters and provides evidence for the potential regulatory mechanism of shell color in oysters. |
format |
Article in Journal/Newspaper |
author |
Jiang, Kunyin Xu, Chengxun Yu, Hong Kong, Lingfeng Liu, Shikai Li, Qi |
author_facet |
Jiang, Kunyin Xu, Chengxun Yu, Hong Kong, Lingfeng Liu, Shikai Li, Qi |
author_sort |
Jiang, Kunyin |
title |
Transcriptomic and Physiological Analysis Reveal Melanin Synthesis-Related Genes and Pathways in Pacific Oysters (Crassostrea gigas). |
title_short |
Transcriptomic and Physiological Analysis Reveal Melanin Synthesis-Related Genes and Pathways in Pacific Oysters (Crassostrea gigas). |
title_full |
Transcriptomic and Physiological Analysis Reveal Melanin Synthesis-Related Genes and Pathways in Pacific Oysters (Crassostrea gigas). |
title_fullStr |
Transcriptomic and Physiological Analysis Reveal Melanin Synthesis-Related Genes and Pathways in Pacific Oysters (Crassostrea gigas). |
title_full_unstemmed |
Transcriptomic and Physiological Analysis Reveal Melanin Synthesis-Related Genes and Pathways in Pacific Oysters (Crassostrea gigas). |
title_sort |
transcriptomic and physiological analysis reveal melanin synthesis-related genes and pathways in pacific oysters (crassostrea gigas). |
publisher |
Springer |
publishDate |
2024 |
url |
https://doi.org/10.1007/s10126-024-10302-2 https://pubmed.ncbi.nlm.nih.gov/38483671 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Mar Biotechnol (NY) ISSN:1436-2236 Volume:26 Issue:2 |
op_relation |
https://doi.org/10.1007/s10126-024-10302-2 https://pubmed.ncbi.nlm.nih.gov/38483671 |
op_rights |
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. |
op_doi |
https://doi.org/10.1007/s10126-024-10302-2 |
container_title |
Marine Biotechnology |
container_volume |
26 |
container_issue |
2 |
container_start_page |
364 |
op_container_end_page |
379 |
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1800750438649167872 |