Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation.
The calcium-binding capacity and osteoblast proliferation and differentiation were studied in Alaska pollock surimi hydrolysate (APSH) using a system that mimics the gastrointestinal digestive system. Evaluation of the calcium absorption-promoting ability of APSH revealed that the best calcium-bindi...
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Online Access: | https://doi.org/10.1111/1750-3841.16988 https://pubmed.ncbi.nlm.nih.gov/38369943 |
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ftpubmed:38369943 2024-05-12T07:52:17+00:00 Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation. Eom, TaeKil Nam, Taek-Jeong 2024 Apr https://doi.org/10.1111/1750-3841.16988 https://pubmed.ncbi.nlm.nih.gov/38369943 eng eng Wiley https://doi.org/10.1111/1750-3841.16988 https://pubmed.ncbi.nlm.nih.gov/38369943 © 2024 The Authors. Journal of Food Science published by Wiley Periodicals LLC on behalf of Institute of Food Technologists. J Food Sci ISSN:1750-3841 Volume:89 Issue:4 Proteinhydrolysate calcium‐binding peptide osteogenesis Journal Article 2024 ftpubmed https://doi.org/10.1111/1750-3841.16988 2024-04-12T16:03:00Z The calcium-binding capacity and osteoblast proliferation and differentiation were studied in Alaska pollock surimi hydrolysate (APSH) using a system that mimics the gastrointestinal digestive system. Evaluation of the calcium absorption-promoting ability of APSH revealed that the best calcium-binding ability was achieved after hydrolysis with a combination of pepsin, α-chymotrypsin, and trypsin, and separation into <3 kDa (APSH-I), 3-5 kDa (APSH-II), 5-10 kDa (APSH-III), and <10 kDa (APSH-IV) fractions. Scanning electron microscopy with energy-dispersive X-ray spectroscopy analysis confirmed that the hydrolysate and calcium ions formed a complex. Comparison of the calcium absorption capacity using Caco-2 cells showed that calcium absorption was promoted by these hydrolysates. Measurement of the osteoblast activation revealed higher alkaline phosphatase activity, collagen synthesis, and mineralization effect for the low-molecular-weight hydrolysate (LMH) than for the other hydrolysates. In addition, LMH promoted the expression of osteocalcin, osteopontin, and bone morphogenetic protein-2 and -4, which are hormones related to bone formation. Expression of the Runx2 transcription factor, which regulates the expression of these hormones, also increased. These results suggest that Alaska pollock surimi protein hydrolysates prepared using a system that mimics gastrointestinal hydrolysis may result in better osteoblast proliferation and bone health than those prepared using other proteases. Article in Journal/Newspaper alaska pollock Alaska PubMed Central (PMC) Journal of Food Science 89 4 2482 2493 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Proteinhydrolysate calcium‐binding peptide osteogenesis |
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Proteinhydrolysate calcium‐binding peptide osteogenesis Eom, TaeKil Nam, Taek-Jeong Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation. |
topic_facet |
Proteinhydrolysate calcium‐binding peptide osteogenesis |
description |
The calcium-binding capacity and osteoblast proliferation and differentiation were studied in Alaska pollock surimi hydrolysate (APSH) using a system that mimics the gastrointestinal digestive system. Evaluation of the calcium absorption-promoting ability of APSH revealed that the best calcium-binding ability was achieved after hydrolysis with a combination of pepsin, α-chymotrypsin, and trypsin, and separation into <3 kDa (APSH-I), 3-5 kDa (APSH-II), 5-10 kDa (APSH-III), and <10 kDa (APSH-IV) fractions. Scanning electron microscopy with energy-dispersive X-ray spectroscopy analysis confirmed that the hydrolysate and calcium ions formed a complex. Comparison of the calcium absorption capacity using Caco-2 cells showed that calcium absorption was promoted by these hydrolysates. Measurement of the osteoblast activation revealed higher alkaline phosphatase activity, collagen synthesis, and mineralization effect for the low-molecular-weight hydrolysate (LMH) than for the other hydrolysates. In addition, LMH promoted the expression of osteocalcin, osteopontin, and bone morphogenetic protein-2 and -4, which are hormones related to bone formation. Expression of the Runx2 transcription factor, which regulates the expression of these hormones, also increased. These results suggest that Alaska pollock surimi protein hydrolysates prepared using a system that mimics gastrointestinal hydrolysis may result in better osteoblast proliferation and bone health than those prepared using other proteases. |
format |
Article in Journal/Newspaper |
author |
Eom, TaeKil Nam, Taek-Jeong |
author_facet |
Eom, TaeKil Nam, Taek-Jeong |
author_sort |
Eom, TaeKil |
title |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation. |
title_short |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation. |
title_full |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation. |
title_fullStr |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation. |
title_full_unstemmed |
Calcium absorption by Alaska pollock surimi protein hydrolysate promotes osteoblast differentiation. |
title_sort |
calcium absorption by alaska pollock surimi protein hydrolysate promotes osteoblast differentiation. |
publisher |
Wiley |
publishDate |
2024 |
url |
https://doi.org/10.1111/1750-3841.16988 https://pubmed.ncbi.nlm.nih.gov/38369943 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_source |
J Food Sci ISSN:1750-3841 Volume:89 Issue:4 |
op_relation |
https://doi.org/10.1111/1750-3841.16988 https://pubmed.ncbi.nlm.nih.gov/38369943 |
op_rights |
© 2024 The Authors. Journal of Food Science published by Wiley Periodicals LLC on behalf of Institute of Food Technologists. |
op_doi |
https://doi.org/10.1111/1750-3841.16988 |
container_title |
Journal of Food Science |
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89 |
container_issue |
4 |
container_start_page |
2482 |
op_container_end_page |
2493 |
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1798851445091467264 |