Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore.
DNA demethylase (DML) is involved in plant development and responses to biotic and abiotic stresses; however, its role in plant-herbivore interaction remains elusive. Here, we found that herbivory by the potato tuber moth, Phthorimaea operculella, rapidly induced the genome-wide DNA methylation and...
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ftpubmed:37781866 2024-09-15T18:03:46+00:00 Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore. Zhang, Yadong Zhong, Jian Munawar, Asim Cai, Yajie He, Wenjing Zhang, Yixin Guo, Han Gao, Yulin Zhu, Zengrong Zhou, Wenwu 2024-01-01 https://doi.org/10.1093/jxb/erad387 https://pubmed.ncbi.nlm.nih.gov/37781866 eng eng Silverchair Information Systems https://doi.org/10.1093/jxb/erad387 https://pubmed.ncbi.nlm.nih.gov/37781866 © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com. J Exp Bot ISSN:1460-2431 Volume:75 Issue:1 Phthorimaea operculella Solanum tuberosum Demeter-Like DNA demethylase jasmonate plant herbivore defense volatile organic compounds Journal Article Research Support, Non-U.S. Gov't 2024 ftpubmed https://doi.org/10.1093/jxb/erad387 2024-07-26T16:03:00Z DNA demethylase (DML) is involved in plant development and responses to biotic and abiotic stresses; however, its role in plant-herbivore interaction remains elusive. Here, we found that herbivory by the potato tuber moth, Phthorimaea operculella, rapidly induced the genome-wide DNA methylation and accumulation of DML gene transcripts in potato plants. Herbivory induction of DML transcripts was suppressed in jasmonate-deficient plants, whereas exogenous application of methyl jasmonate (MeJA) improved DML transcripts, indicating that the induction of DML transcripts by herbivory is associated with jasmonate signaling. Moreover, P. operculella larvae grew heavier on DML gene (StDML2) knockdown plants than on wild-type plants, and the decreased biosynthesis of jasmonates in the former may be responsible for this difference, since the larvae feeding on these two genotypes supplemented with MeJA showed similar growth. In addition, P. operculella adult moths preferred to oviposit on StDML2 knockdown plants than on wild-type plants, which was associated with the reduced emission of β-caryophyllene in the former. In addition, supplementing β-caryophyllene to these two genotypes further disrupted moths' oviposit choice preference for them. Interestingly, in StDML2 knockdown plants, hypermethylation was found at the promoter regions for the key genes StAOS and StAOC in the jasmonate biosynthetic pathway, as well as for the key gene StTPS12 in β-caryophyllene production. Our findings suggest that knocking down StDML2 can affect herbivore defense via jasmonate signaling and defense compound production in potato plants. Article in Journal/Newspaper DML PubMed Central (PMC) Journal of Experimental Botany 75 1 483 499 |
institution |
Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Phthorimaea operculella Solanum tuberosum Demeter-Like DNA demethylase jasmonate plant herbivore defense volatile organic compounds |
spellingShingle |
Phthorimaea operculella Solanum tuberosum Demeter-Like DNA demethylase jasmonate plant herbivore defense volatile organic compounds Zhang, Yadong Zhong, Jian Munawar, Asim Cai, Yajie He, Wenjing Zhang, Yixin Guo, Han Gao, Yulin Zhu, Zengrong Zhou, Wenwu Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore. |
topic_facet |
Phthorimaea operculella Solanum tuberosum Demeter-Like DNA demethylase jasmonate plant herbivore defense volatile organic compounds |
description |
DNA demethylase (DML) is involved in plant development and responses to biotic and abiotic stresses; however, its role in plant-herbivore interaction remains elusive. Here, we found that herbivory by the potato tuber moth, Phthorimaea operculella, rapidly induced the genome-wide DNA methylation and accumulation of DML gene transcripts in potato plants. Herbivory induction of DML transcripts was suppressed in jasmonate-deficient plants, whereas exogenous application of methyl jasmonate (MeJA) improved DML transcripts, indicating that the induction of DML transcripts by herbivory is associated with jasmonate signaling. Moreover, P. operculella larvae grew heavier on DML gene (StDML2) knockdown plants than on wild-type plants, and the decreased biosynthesis of jasmonates in the former may be responsible for this difference, since the larvae feeding on these two genotypes supplemented with MeJA showed similar growth. In addition, P. operculella adult moths preferred to oviposit on StDML2 knockdown plants than on wild-type plants, which was associated with the reduced emission of β-caryophyllene in the former. In addition, supplementing β-caryophyllene to these two genotypes further disrupted moths' oviposit choice preference for them. Interestingly, in StDML2 knockdown plants, hypermethylation was found at the promoter regions for the key genes StAOS and StAOC in the jasmonate biosynthetic pathway, as well as for the key gene StTPS12 in β-caryophyllene production. Our findings suggest that knocking down StDML2 can affect herbivore defense via jasmonate signaling and defense compound production in potato plants. |
format |
Article in Journal/Newspaper |
author |
Zhang, Yadong Zhong, Jian Munawar, Asim Cai, Yajie He, Wenjing Zhang, Yixin Guo, Han Gao, Yulin Zhu, Zengrong Zhou, Wenwu |
author_facet |
Zhang, Yadong Zhong, Jian Munawar, Asim Cai, Yajie He, Wenjing Zhang, Yixin Guo, Han Gao, Yulin Zhu, Zengrong Zhou, Wenwu |
author_sort |
Zhang, Yadong |
title |
Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore. |
title_short |
Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore. |
title_full |
Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore. |
title_fullStr |
Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore. |
title_full_unstemmed |
Knocking down a DNA demethylase gene affects potato plant defense against a specialist insect herbivore. |
title_sort |
knocking down a dna demethylase gene affects potato plant defense against a specialist insect herbivore. |
publisher |
Silverchair Information Systems |
publishDate |
2024 |
url |
https://doi.org/10.1093/jxb/erad387 https://pubmed.ncbi.nlm.nih.gov/37781866 |
genre |
DML |
genre_facet |
DML |
op_source |
J Exp Bot ISSN:1460-2431 Volume:75 Issue:1 |
op_relation |
https://doi.org/10.1093/jxb/erad387 https://pubmed.ncbi.nlm.nih.gov/37781866 |
op_rights |
© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com. |
op_doi |
https://doi.org/10.1093/jxb/erad387 |
container_title |
Journal of Experimental Botany |
container_volume |
75 |
container_issue |
1 |
container_start_page |
483 |
op_container_end_page |
499 |
_version_ |
1810441236902838272 |