Biocatalytic quantification of α-glucan in marine particulate organic matter.

• Published in: MicrobiologyOpen
• Main Authors: Steinke, Nicola, Vidal-Melgosa, Silvia, Schultz-Johansen, Mikkel, Hehemann, Jan-Hendrik
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2022
• Subjects: algae; enzymatic hydrolysis; glucans; marine particulate organic matter; polysaccharides; quantification; North Atlantic
• Online Access: https://doi.org/10.1002/mbo3.1289; https://pubmed.ncbi.nlm.nih.gov/35765187; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134812/

Marine algae drive the marine carbon cycle, converting carbon dioxide into organic material. A major component of this produced biomass is a variety of glycans. Marine α-glucans include a range of storage glycans from red and green algae, bacteria, fungi, and animals. Although these compounds are likely to account for a high amount of the carbon stored in the oceans they have not been quantified in marine samples so far. Here we present a method to extract and quantify α-glucans (and compare it with the β-glucan laminarin) in particulate organic matter from algal cultures and environmental samples using sequential physicochemical extraction and enzymes as α-glucan-specific probes. This enzymatic assay is more specific and less susceptible to side reactions than chemical hydrolysis. Using HPAEC-PAD to detect the hydrolysis products allows for a glycan quantification in particulate marine samples down to a concentration of ≈2 µg/L. We measured glucans in three cultured microalgae as well as in marine particulate organic matter from the North Sea and western North Atlantic Ocean. While the β-glucan laminarin from diatoms and brown algae is an essential component of marine carbon turnover, our results further indicate the significant contribution of starch-like α-glucans to marine particulate organic matter. Henceforth, the combination of glycan-linkage-specific enzymes and chromatographic hydrolysis product detection can provide a powerful tool in the exploration of marine glycans and their role in the global carbon cycle.