Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis.
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of...
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ftpubmed:35614207 2024-09-15T18:35:26+00:00 Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis. Freyria, Nastasia J Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W Grigoriev, Igor V Lovejoy, Connie 2022-05-25 https://doi.org/10.1038/s42003-022-03461-2 https://pubmed.ncbi.nlm.nih.gov/35614207 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ eng eng Nature Publishing Group https://doi.org/10.1038/s42003-022-03461-2 https://pubmed.ncbi.nlm.nih.gov/35614207 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ © 2022. The Author(s). Commun Biol ISSN:2399-3642 Volume:5 Issue:1 Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't 2022 ftpubmed https://doi.org/10.1038/s42003-022-03461-2 2024-08-28T16:03:00Z Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na+-H+ antiporters and Na+ -Pi symporters as salinity decreases, but the K+ channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. Article in Journal/Newspaper Sea ice PubMed Central (PMC) Communications Biology 5 1 |
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English |
description |
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na+-H+ antiporters and Na+ -Pi symporters as salinity decreases, but the K+ channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. |
format |
Article in Journal/Newspaper |
author |
Freyria, Nastasia J Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W Grigoriev, Igor V Lovejoy, Connie |
spellingShingle |
Freyria, Nastasia J Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W Grigoriev, Igor V Lovejoy, Connie Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis. |
author_facet |
Freyria, Nastasia J Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W Grigoriev, Igor V Lovejoy, Connie |
author_sort |
Freyria, Nastasia J |
title |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis. |
title_short |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis. |
title_full |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis. |
title_fullStr |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis. |
title_full_unstemmed |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis. |
title_sort |
salinity tolerance mechanisms of an arctic pelagophyte using comparative transcriptomic and gene expression analysis. |
publisher |
Nature Publishing Group |
publishDate |
2022 |
url |
https://doi.org/10.1038/s42003-022-03461-2 https://pubmed.ncbi.nlm.nih.gov/35614207 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ |
genre |
Sea ice |
genre_facet |
Sea ice |
op_source |
Commun Biol ISSN:2399-3642 Volume:5 Issue:1 |
op_relation |
https://doi.org/10.1038/s42003-022-03461-2 https://pubmed.ncbi.nlm.nih.gov/35614207 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133084/ |
op_rights |
© 2022. The Author(s). |
op_doi |
https://doi.org/10.1038/s42003-022-03461-2 |
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Communications Biology |
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5 |
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1 |
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1810478618111901696 |