Green and efficient biosynthesis of indigo from indole by engineered myoglobins.
With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglob...
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ftpubmed:35548150 2024-09-15T18:37:35+00:00 Green and efficient biosynthesis of indigo from indole by engineered myoglobins. Liu, Can Xu, Jiakun Gao, Shu-Qin He, Bo Wei, Chuan-Wan Wang, Xiao-Juan Wang, Zhonghua Lin, Ying-Wu 2018 Sep 24 https://doi.org/10.1039/c8ra07825d https://pubmed.ncbi.nlm.nih.gov/35548150 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086478/ eng eng PubMed Central https://doi.org/10.1039/c8ra07825d https://pubmed.ncbi.nlm.nih.gov/35548150 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086478/ This journal is © The Royal Society of Chemistry. RSC Adv ISSN:2046-2069 Volume:8 Issue:58 Journal Article 2018 ftpubmed https://doi.org/10.1039/c8ra07825d 2024-08-27T16:03:00Z With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglobin, F43Y Mb, with a novel Tyr-heme cross-link. In this study, we found that it can efficiently catalyze the oxidation of indole to indigo, with a yield as high as 54% compared to the highest yield (∼20%) reported to date in the literature. By further modifying the heme active site, we engineered a double mutant of F43Y/H64D Mb, which exhibited the highest catalytic efficiency (198 M-1 s-1) among the artificial enzymes designed in Mb. Moreover, both F43Y Mb and F43Y/H64D Mb were found to produce the indigo product with a chemoselectivity as high as ∼80%. Based on the reaction system, we also established a convenient and green dyeing method by dyeing a cotton textile during the biosynthesis of indigo, followed by further spraying the concentrated indigo, without the need of strong acids/bases or any reducing agents. The successful application of dyeing a white cotton textile with a blue color further indicates that the designed enzyme and the dyeing method have practical applications in the future. Article in Journal/Newspaper Sperm whale PubMed Central (PMC) RSC Advances 8 58 33325 33330 |
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With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglobin, F43Y Mb, with a novel Tyr-heme cross-link. In this study, we found that it can efficiently catalyze the oxidation of indole to indigo, with a yield as high as 54% compared to the highest yield (∼20%) reported to date in the literature. By further modifying the heme active site, we engineered a double mutant of F43Y/H64D Mb, which exhibited the highest catalytic efficiency (198 M-1 s-1) among the artificial enzymes designed in Mb. Moreover, both F43Y Mb and F43Y/H64D Mb were found to produce the indigo product with a chemoselectivity as high as ∼80%. Based on the reaction system, we also established a convenient and green dyeing method by dyeing a cotton textile during the biosynthesis of indigo, followed by further spraying the concentrated indigo, without the need of strong acids/bases or any reducing agents. The successful application of dyeing a white cotton textile with a blue color further indicates that the designed enzyme and the dyeing method have practical applications in the future. |
format |
Article in Journal/Newspaper |
author |
Liu, Can Xu, Jiakun Gao, Shu-Qin He, Bo Wei, Chuan-Wan Wang, Xiao-Juan Wang, Zhonghua Lin, Ying-Wu |
spellingShingle |
Liu, Can Xu, Jiakun Gao, Shu-Qin He, Bo Wei, Chuan-Wan Wang, Xiao-Juan Wang, Zhonghua Lin, Ying-Wu Green and efficient biosynthesis of indigo from indole by engineered myoglobins. |
author_facet |
Liu, Can Xu, Jiakun Gao, Shu-Qin He, Bo Wei, Chuan-Wan Wang, Xiao-Juan Wang, Zhonghua Lin, Ying-Wu |
author_sort |
Liu, Can |
title |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins. |
title_short |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins. |
title_full |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins. |
title_fullStr |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins. |
title_full_unstemmed |
Green and efficient biosynthesis of indigo from indole by engineered myoglobins. |
title_sort |
green and efficient biosynthesis of indigo from indole by engineered myoglobins. |
publisher |
PubMed Central |
publishDate |
2018 |
url |
https://doi.org/10.1039/c8ra07825d https://pubmed.ncbi.nlm.nih.gov/35548150 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086478/ |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
RSC Adv ISSN:2046-2069 Volume:8 Issue:58 |
op_relation |
https://doi.org/10.1039/c8ra07825d https://pubmed.ncbi.nlm.nih.gov/35548150 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086478/ |
op_rights |
This journal is © The Royal Society of Chemistry. |
op_doi |
https://doi.org/10.1039/c8ra07825d |
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58 |
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33325 |
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33330 |
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1810481947689877504 |