Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors.
Edwardsiella piscicida causes edwardsiellosis in a variety of fish species and leads to tremendous economic losses in the global aquaculture industries. Thus, effective and safe prevention and control of this bacterium are urgently needed to combat the related infections. Live attenuated vaccines (L...
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Online Access: | https://doi.org/10.1016/j.fsi.2020.07.021 https://pubmed.ncbi.nlm.nih.gov/32763422 |
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ftpubmed:32763422 2024-06-09T07:50:03+00:00 Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors. Yin, Kaiyu Ma, Jiabao Jin, Peng Sun, Xiang Liu, Xiaohong Wang, Qiyao 2020 Nov https://doi.org/10.1016/j.fsi.2020.07.021 https://pubmed.ncbi.nlm.nih.gov/32763422 eng eng Elsevier Science https://doi.org/10.1016/j.fsi.2020.07.021 https://pubmed.ncbi.nlm.nih.gov/32763422 Copyright © 2020 Elsevier Ltd. All rights reserved. Fish Shellfish Immunol ISSN:1095-9947 Volume:106 Adaptive immune response Edwardsiella piscicida Effectors LAV T3SS Journal Article 2020 ftpubmed https://doi.org/10.1016/j.fsi.2020.07.021 2024-05-12T16:03:00Z Edwardsiella piscicida causes edwardsiellosis in a variety of fish species and leads to tremendous economic losses in the global aquaculture industries. Thus, effective and safe prevention and control of this bacterium are urgently needed to combat the related infections. Live attenuated vaccines (LAVs) effectively prevent infectious diseases. However, most of the existing E. piscicida LAVs are based on the deletion of genes encoding the translocon components of the type III secretion system (T3SS), the core virulence system, which is the most prominent protective bacterial antigen with the strongest immunogenicity. In this study, we systematically deleted all of the 9 established T3SS effectors in E. piscicida (aka 9Δ) and the rpoS gene encoding the alternative sigma factor, the esrB repressor (10Δ), then we overexpressed esrB and T3SS in E. piscicida to obtain the recombinant strain 10Δ/esrBOE. The modified strains 10Δ and 10Δ/esrBOE exhibited severe attenuation and in vivo colonization defects. Additionally, vaccination by intraperitoneal injection with 10Δ and 10Δ/esrBOE could significantly upregulate the expression of the antigen recognition related gene (TLR5) and the adaptive immune response-related gene (MHC II) in the spleen/kidney of turbot fish, and it also enhanced the hosts' serum bactericidal capacity. Finally, vaccination with 10Δ/esrBOE led to increased immune protection against the challenge of wild type E. piscicida EIB202 in turbot fish. Collectively, these findings demonstrated that 10Δ/esrBOE was a novel LAV strain and therefore a potential novel strategy for the construction of LAVs against bacterial pathogens. Article in Journal/Newspaper Turbot PubMed Central (PMC) Fish & Shellfish Immunology 106 536 545 |
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Open Polar |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Adaptive immune response Edwardsiella piscicida Effectors LAV T3SS |
spellingShingle |
Adaptive immune response Edwardsiella piscicida Effectors LAV T3SS Yin, Kaiyu Ma, Jiabao Jin, Peng Sun, Xiang Liu, Xiaohong Wang, Qiyao Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors. |
topic_facet |
Adaptive immune response Edwardsiella piscicida Effectors LAV T3SS |
description |
Edwardsiella piscicida causes edwardsiellosis in a variety of fish species and leads to tremendous economic losses in the global aquaculture industries. Thus, effective and safe prevention and control of this bacterium are urgently needed to combat the related infections. Live attenuated vaccines (LAVs) effectively prevent infectious diseases. However, most of the existing E. piscicida LAVs are based on the deletion of genes encoding the translocon components of the type III secretion system (T3SS), the core virulence system, which is the most prominent protective bacterial antigen with the strongest immunogenicity. In this study, we systematically deleted all of the 9 established T3SS effectors in E. piscicida (aka 9Δ) and the rpoS gene encoding the alternative sigma factor, the esrB repressor (10Δ), then we overexpressed esrB and T3SS in E. piscicida to obtain the recombinant strain 10Δ/esrBOE. The modified strains 10Δ and 10Δ/esrBOE exhibited severe attenuation and in vivo colonization defects. Additionally, vaccination by intraperitoneal injection with 10Δ and 10Δ/esrBOE could significantly upregulate the expression of the antigen recognition related gene (TLR5) and the adaptive immune response-related gene (MHC II) in the spleen/kidney of turbot fish, and it also enhanced the hosts' serum bactericidal capacity. Finally, vaccination with 10Δ/esrBOE led to increased immune protection against the challenge of wild type E. piscicida EIB202 in turbot fish. Collectively, these findings demonstrated that 10Δ/esrBOE was a novel LAV strain and therefore a potential novel strategy for the construction of LAVs against bacterial pathogens. |
format |
Article in Journal/Newspaper |
author |
Yin, Kaiyu Ma, Jiabao Jin, Peng Sun, Xiang Liu, Xiaohong Wang, Qiyao |
author_facet |
Yin, Kaiyu Ma, Jiabao Jin, Peng Sun, Xiang Liu, Xiaohong Wang, Qiyao |
author_sort |
Yin, Kaiyu |
title |
Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors. |
title_short |
Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors. |
title_full |
Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors. |
title_fullStr |
Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors. |
title_full_unstemmed |
Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors. |
title_sort |
characterization of a novel live attenuated edwardsiella piscicida vaccine based on the overexpressed type iii secretion system and systematic deletion of the associated effectors. |
publisher |
Elsevier Science |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.fsi.2020.07.021 https://pubmed.ncbi.nlm.nih.gov/32763422 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Fish Shellfish Immunol ISSN:1095-9947 Volume:106 |
op_relation |
https://doi.org/10.1016/j.fsi.2020.07.021 https://pubmed.ncbi.nlm.nih.gov/32763422 |
op_rights |
Copyright © 2020 Elsevier Ltd. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.fsi.2020.07.021 |
container_title |
Fish & Shellfish Immunology |
container_volume |
106 |
container_start_page |
536 |
op_container_end_page |
545 |
_version_ |
1801383036246294528 |