Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel.
Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergi...
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ftpubmed:32634160 2024-09-15T17:39:46+00:00 Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel. Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna 2020 https://doi.org/10.1371/journal.pone.0235617 https://pubmed.ncbi.nlm.nih.gov/32634160 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340298/ eng eng Public Library of Science https://doi.org/10.1371/journal.pone.0235617 https://pubmed.ncbi.nlm.nih.gov/32634160 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340298/ PLoS One ISSN:1932-6203 Volume:15 Issue:7 Journal Article Research Support, Non-U.S. Gov't 2020 ftpubmed https://doi.org/10.1371/journal.pone.0235617 2024-07-30T16:03:00Z Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergic inhibition of the neuroendocrine system, preventing gonadotropin secretion and gonadal development. The present study used either CPE or SPE to induce vitellogenesis in female European eel and compared impacts on egg quality and offspring developmental competence with emphasis on the maternal-to-zygotic transition (MZT). Females treated with SPE produced significantly higher proportions of floating eggs with fewer cleavage abnormalities and higher embryonic survival. These findings related successful embryogenesis to higher abundance of mRNA transcripts of genes involved in cell adhesion, activation of MZT, and immune response (dcbld1, epcam, oct4, igm) throughout embryonic development. The abundance of mRNA transcripts of cldnd, foxr1, cea, ccna1, ccnb1, ccnb2, zar1, oct4, and npm2 was relatively stable during the first eight hours, followed by a drop during MZT and low levels thereafter, indicating transfer and subsequent clearance of maternal mRNA. mRNA abundance of zar1, epcam, and dicer1 was associated with cleavage abnormalities, while mRNA abundance of zar1, sox2, foxr1, cldnd, phb2, neurod4, and neurog1 (before MZT) was associated with subsequent embryonic survival. In a second pattern, low initial mRNA abundance with an increase during MZT and higher levels persisting thereafter indicating the activation of zygotic transcription. mRNA abundance of ccna1, npm2, oct4, neurod4, and neurog1 during later embryonic development was associated with hatch success. A deviating pattern was observed for dcbld1, which mRNA levels followed the maternal-effect gene pattern but only for embryos from SPE treated females. Together, the differences in offspring production and performance reported in this study show that PE composition impacts egg quality and embryogenesis and in particular, the transition from initial maternal transcripts to zygotic transcription. Article in Journal/Newspaper Anguilla anguilla PubMed Central (PMC) PLOS ONE 15 7 e0235617 |
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PubMed Central (PMC) |
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English |
description |
Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergic inhibition of the neuroendocrine system, preventing gonadotropin secretion and gonadal development. The present study used either CPE or SPE to induce vitellogenesis in female European eel and compared impacts on egg quality and offspring developmental competence with emphasis on the maternal-to-zygotic transition (MZT). Females treated with SPE produced significantly higher proportions of floating eggs with fewer cleavage abnormalities and higher embryonic survival. These findings related successful embryogenesis to higher abundance of mRNA transcripts of genes involved in cell adhesion, activation of MZT, and immune response (dcbld1, epcam, oct4, igm) throughout embryonic development. The abundance of mRNA transcripts of cldnd, foxr1, cea, ccna1, ccnb1, ccnb2, zar1, oct4, and npm2 was relatively stable during the first eight hours, followed by a drop during MZT and low levels thereafter, indicating transfer and subsequent clearance of maternal mRNA. mRNA abundance of zar1, epcam, and dicer1 was associated with cleavage abnormalities, while mRNA abundance of zar1, sox2, foxr1, cldnd, phb2, neurod4, and neurog1 (before MZT) was associated with subsequent embryonic survival. In a second pattern, low initial mRNA abundance with an increase during MZT and higher levels persisting thereafter indicating the activation of zygotic transcription. mRNA abundance of ccna1, npm2, oct4, neurod4, and neurog1 during later embryonic development was associated with hatch success. A deviating pattern was observed for dcbld1, which mRNA levels followed the maternal-effect gene pattern but only for embryos from SPE treated females. Together, the differences in offspring production and performance reported in this study show that PE composition impacts egg quality and embryogenesis and in particular, the transition from initial maternal transcripts to zygotic transcription. |
format |
Article in Journal/Newspaper |
author |
Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna |
spellingShingle |
Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel. |
author_facet |
Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna |
author_sort |
Kottmann, Johanna S |
title |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel. |
title_short |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel. |
title_full |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel. |
title_fullStr |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel. |
title_full_unstemmed |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel. |
title_sort |
differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in european eel. |
publisher |
Public Library of Science |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pone.0235617 https://pubmed.ncbi.nlm.nih.gov/32634160 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340298/ |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
PLoS One ISSN:1932-6203 Volume:15 Issue:7 |
op_relation |
https://doi.org/10.1371/journal.pone.0235617 https://pubmed.ncbi.nlm.nih.gov/32634160 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340298/ |
op_doi |
https://doi.org/10.1371/journal.pone.0235617 |
container_title |
PLOS ONE |
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15 |
container_issue |
7 |
container_start_page |
e0235617 |
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1810482385710481408 |