Anisakid nematode species identification in harbour porpoises (
Harbour porpoises (Phocoena phocoena) are the only native cetacean species in the German North and Baltic Seas and the final host of Anisakis (A.) simplex, which infects their first and second gastric compartments and may cause chronic ulcerative gastritis. Anisakis simplex belongs to the family Ani...
Published in: | International Journal for Parasitology: Parasites and Wildlife |
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Online Access: | https://doi.org/10.1016/j.ijppaw.2020.05.004 https://pubmed.ncbi.nlm.nih.gov/32489854 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260678/ |
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ftpubmed:32489854 2024-09-15T18:10:43+00:00 Anisakid nematode species identification in harbour porpoises ( Lakemeyer, Jan Siebert, Ursula Abdulmawjood, Amir Ryeng, Kathrine A IJsseldijk, Lonneke L Lehnert, Kristina 2020 Aug https://doi.org/10.1016/j.ijppaw.2020.05.004 https://pubmed.ncbi.nlm.nih.gov/32489854 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260678/ eng eng PubMed Central https://doi.org/10.1016/j.ijppaw.2020.05.004 https://pubmed.ncbi.nlm.nih.gov/32489854 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260678/ © 2020 Published by Elsevier Ltd on behalf of Australian Society for Parasitology. Int J Parasitol Parasites Wildl ISSN:2213-2244 Volume:12 Anisakid nematodes Anisakis simplex Contracaecum osculatum Harbour porpoise (Phocoena phocoena) Hysterothylacium aduncum Pseudoterranova decipiens Restriction fragment length polymorphism (RFLP) Journal Article 2020 ftpubmed https://doi.org/10.1016/j.ijppaw.2020.05.004 2024-07-29T16:03:00Z Harbour porpoises (Phocoena phocoena) are the only native cetacean species in the German North and Baltic Seas and the final host of Anisakis (A.) simplex, which infects their first and second gastric compartments and may cause chronic ulcerative gastritis. Anisakis simplex belongs to the family Anisakidae (Ascaridoidea, Rhabditida) as well as the phocine gastric nematode species Pseudoterranova (P.) decipiens and Contracaecum (C.) osculatum. These nematode species are the main causative agents for the zoonosis anisakidosis. The taxonomy of these genus with life cycles including crustaceans and commercially important fish is complex because of the formation of sibling species. Little is known about anisakid species infecting porpoises in the study area. Mature nematodes and larval stages are often identifiable only by molecular methods due to high morphological and genetic similarity. The restriction fragment length polymorphism (RFLP) method is an alternative to sequencing and was applied to identify anisakid nematodes found in harbour porpoises from the North Sea, Baltic Sea and North Atlantic to species level for the first time. In the study areas, five gastric nematodes from different harbour porpoise hosts were selected to be investigated with restriction enzymes HinfI, RsaI and HaeIII, which were able to differentiate several anisakid nematode species by characteristic banding patterns. Anisakis simplex s. s. was the dominant species found in the North Sea and Baltic porpoises, identified by all three restriction enzymes. Additionally, a hybrid of A. simplex s. s. and A. pegreffii was determined by HinfI in the North Sea samples. Within the North Atlantic specimens, A. simplex s. s., P. decipiens s. s. and Hysterothylacium (H.) aduncum were identified by all enzymes. This demonstrates the value of the RFLP method and the chosen restriction enzymes for the species identification of a broad variety of anisakid nematodes affecting the health of marine mammals. Article in Journal/Newspaper Harbour porpoise North Atlantic Phocoena phocoena PubMed Central (PMC) International Journal for Parasitology: Parasites and Wildlife 12 93 98 |
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Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Anisakid nematodes Anisakis simplex Contracaecum osculatum Harbour porpoise (Phocoena phocoena) Hysterothylacium aduncum Pseudoterranova decipiens Restriction fragment length polymorphism (RFLP) |
spellingShingle |
Anisakid nematodes Anisakis simplex Contracaecum osculatum Harbour porpoise (Phocoena phocoena) Hysterothylacium aduncum Pseudoterranova decipiens Restriction fragment length polymorphism (RFLP) Lakemeyer, Jan Siebert, Ursula Abdulmawjood, Amir Ryeng, Kathrine A IJsseldijk, Lonneke L Lehnert, Kristina Anisakid nematode species identification in harbour porpoises ( |
topic_facet |
Anisakid nematodes Anisakis simplex Contracaecum osculatum Harbour porpoise (Phocoena phocoena) Hysterothylacium aduncum Pseudoterranova decipiens Restriction fragment length polymorphism (RFLP) |
description |
Harbour porpoises (Phocoena phocoena) are the only native cetacean species in the German North and Baltic Seas and the final host of Anisakis (A.) simplex, which infects their first and second gastric compartments and may cause chronic ulcerative gastritis. Anisakis simplex belongs to the family Anisakidae (Ascaridoidea, Rhabditida) as well as the phocine gastric nematode species Pseudoterranova (P.) decipiens and Contracaecum (C.) osculatum. These nematode species are the main causative agents for the zoonosis anisakidosis. The taxonomy of these genus with life cycles including crustaceans and commercially important fish is complex because of the formation of sibling species. Little is known about anisakid species infecting porpoises in the study area. Mature nematodes and larval stages are often identifiable only by molecular methods due to high morphological and genetic similarity. The restriction fragment length polymorphism (RFLP) method is an alternative to sequencing and was applied to identify anisakid nematodes found in harbour porpoises from the North Sea, Baltic Sea and North Atlantic to species level for the first time. In the study areas, five gastric nematodes from different harbour porpoise hosts were selected to be investigated with restriction enzymes HinfI, RsaI and HaeIII, which were able to differentiate several anisakid nematode species by characteristic banding patterns. Anisakis simplex s. s. was the dominant species found in the North Sea and Baltic porpoises, identified by all three restriction enzymes. Additionally, a hybrid of A. simplex s. s. and A. pegreffii was determined by HinfI in the North Sea samples. Within the North Atlantic specimens, A. simplex s. s., P. decipiens s. s. and Hysterothylacium (H.) aduncum were identified by all enzymes. This demonstrates the value of the RFLP method and the chosen restriction enzymes for the species identification of a broad variety of anisakid nematodes affecting the health of marine mammals. |
format |
Article in Journal/Newspaper |
author |
Lakemeyer, Jan Siebert, Ursula Abdulmawjood, Amir Ryeng, Kathrine A IJsseldijk, Lonneke L Lehnert, Kristina |
author_facet |
Lakemeyer, Jan Siebert, Ursula Abdulmawjood, Amir Ryeng, Kathrine A IJsseldijk, Lonneke L Lehnert, Kristina |
author_sort |
Lakemeyer, Jan |
title |
Anisakid nematode species identification in harbour porpoises ( |
title_short |
Anisakid nematode species identification in harbour porpoises ( |
title_full |
Anisakid nematode species identification in harbour porpoises ( |
title_fullStr |
Anisakid nematode species identification in harbour porpoises ( |
title_full_unstemmed |
Anisakid nematode species identification in harbour porpoises ( |
title_sort |
anisakid nematode species identification in harbour porpoises ( |
publisher |
PubMed Central |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.ijppaw.2020.05.004 https://pubmed.ncbi.nlm.nih.gov/32489854 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260678/ |
genre |
Harbour porpoise North Atlantic Phocoena phocoena |
genre_facet |
Harbour porpoise North Atlantic Phocoena phocoena |
op_source |
Int J Parasitol Parasites Wildl ISSN:2213-2244 Volume:12 |
op_relation |
https://doi.org/10.1016/j.ijppaw.2020.05.004 https://pubmed.ncbi.nlm.nih.gov/32489854 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260678/ |
op_rights |
© 2020 Published by Elsevier Ltd on behalf of Australian Society for Parasitology. |
op_doi |
https://doi.org/10.1016/j.ijppaw.2020.05.004 |
container_title |
International Journal for Parasitology: Parasites and Wildlife |
container_volume |
12 |
container_start_page |
93 |
op_container_end_page |
98 |
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1810448294852165632 |