Erythroid Progenitor Cells in Atlantic Salmon (
Piscine orthoreovirus (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). The virus targets erythrocytes in the acute peak phase, followed by cardiomyocytes, before the infection subsides into persistence. The persistent phase is characterized by...
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ftpubmed:31491892 2024-09-09T19:30:10+00:00 Erythroid Progenitor Cells in Atlantic Salmon ( Malik, Muhammad Salman Bjørgen, Håvard Dhamotharan, Kannimuthu Wessel, Øystein Koppang, Erling Olaf Di Cicco, Emiliano Hansen, Elisabeth F Dahle, Maria K Rimstad, Espen 2019-09-05 https://doi.org/10.3390/v11090824 https://pubmed.ncbi.nlm.nih.gov/31491892 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784031/ eng eng MDPI https://doi.org/10.3390/v11090824 https://pubmed.ncbi.nlm.nih.gov/31491892 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784031/ Viruses ISSN:1999-4915 Volume:11 Issue:9 PRV-1 erythroid progenitor cells persistence piscine orthoreovirus Journal Article Research Support, Non-U.S. Gov't 2019 ftpubmed https://doi.org/10.3390/v11090824 2024-07-20T16:02:00Z Piscine orthoreovirus (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). The virus targets erythrocytes in the acute peak phase, followed by cardiomyocytes, before the infection subsides into persistence. The persistent phase is characterized by high level of viral RNA, but low level of viral protein. The origin and nature of persistent PRV-1 are not clear. Here, we analyzed for viral persistence and activity in various tissues and cell types in experimentally infected Atlantic salmon. Plasma contained PRV-1 genomic dsRNA throughout an 18-week long infection trial, indicating that viral particles are continuously produced and released. The highest level of PRV-1 RNA in the persistent phase was found in kidney. The level of PRV-1 ssRNA transcripts in kidney was significantly higher than that of blood cells in the persistent phase. In-situ hybridization assays confirmed that PRV-1 RNA was present in erythroid progenitor cells, erythrocytes, macrophages, melano-macrophages and in some additional un-characterized cells in kidney. These results show that PRV-1 establishes a productive, persistent infection in Atlantic salmon and that erythrocyte progenitor cells are PRV target cells. Article in Journal/Newspaper Atlantic salmon Salmo salar PubMed Central (PMC) Viruses 11 9 824 |
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PubMed Central (PMC) |
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English |
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PRV-1 erythroid progenitor cells persistence piscine orthoreovirus |
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PRV-1 erythroid progenitor cells persistence piscine orthoreovirus Malik, Muhammad Salman Bjørgen, Håvard Dhamotharan, Kannimuthu Wessel, Øystein Koppang, Erling Olaf Di Cicco, Emiliano Hansen, Elisabeth F Dahle, Maria K Rimstad, Espen Erythroid Progenitor Cells in Atlantic Salmon ( |
topic_facet |
PRV-1 erythroid progenitor cells persistence piscine orthoreovirus |
description |
Piscine orthoreovirus (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). The virus targets erythrocytes in the acute peak phase, followed by cardiomyocytes, before the infection subsides into persistence. The persistent phase is characterized by high level of viral RNA, but low level of viral protein. The origin and nature of persistent PRV-1 are not clear. Here, we analyzed for viral persistence and activity in various tissues and cell types in experimentally infected Atlantic salmon. Plasma contained PRV-1 genomic dsRNA throughout an 18-week long infection trial, indicating that viral particles are continuously produced and released. The highest level of PRV-1 RNA in the persistent phase was found in kidney. The level of PRV-1 ssRNA transcripts in kidney was significantly higher than that of blood cells in the persistent phase. In-situ hybridization assays confirmed that PRV-1 RNA was present in erythroid progenitor cells, erythrocytes, macrophages, melano-macrophages and in some additional un-characterized cells in kidney. These results show that PRV-1 establishes a productive, persistent infection in Atlantic salmon and that erythrocyte progenitor cells are PRV target cells. |
format |
Article in Journal/Newspaper |
author |
Malik, Muhammad Salman Bjørgen, Håvard Dhamotharan, Kannimuthu Wessel, Øystein Koppang, Erling Olaf Di Cicco, Emiliano Hansen, Elisabeth F Dahle, Maria K Rimstad, Espen |
author_facet |
Malik, Muhammad Salman Bjørgen, Håvard Dhamotharan, Kannimuthu Wessel, Øystein Koppang, Erling Olaf Di Cicco, Emiliano Hansen, Elisabeth F Dahle, Maria K Rimstad, Espen |
author_sort |
Malik, Muhammad Salman |
title |
Erythroid Progenitor Cells in Atlantic Salmon ( |
title_short |
Erythroid Progenitor Cells in Atlantic Salmon ( |
title_full |
Erythroid Progenitor Cells in Atlantic Salmon ( |
title_fullStr |
Erythroid Progenitor Cells in Atlantic Salmon ( |
title_full_unstemmed |
Erythroid Progenitor Cells in Atlantic Salmon ( |
title_sort |
erythroid progenitor cells in atlantic salmon ( |
publisher |
MDPI |
publishDate |
2019 |
url |
https://doi.org/10.3390/v11090824 https://pubmed.ncbi.nlm.nih.gov/31491892 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784031/ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Viruses ISSN:1999-4915 Volume:11 Issue:9 |
op_relation |
https://doi.org/10.3390/v11090824 https://pubmed.ncbi.nlm.nih.gov/31491892 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784031/ |
op_doi |
https://doi.org/10.3390/v11090824 |
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Viruses |
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11 |
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824 |
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