The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.).
In the present study, Fourier-transform infrared spectroscopy (FTIR) is investigated as a method to measure connective tissue components that are important for the quality of Atlantic cod filets (Gadus morhua L.). The Atlantic cod used in this study originated from a feeding trial, which found that...
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Online Access: | https://doi.org/10.1002/jbio.201800436 https://pubmed.ncbi.nlm.nih.gov/31162834 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065610/ |
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ftpubmed:31162834 2024-09-09T19:29:40+00:00 The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.). Sanden, Karen W Kohler, Achim Afseth, Nils K Böcker, Ulrike Rønning, Sissel B Liland, Kristian H Pedersen, Mona E 2019-09 https://doi.org/10.1002/jbio.201800436 https://pubmed.ncbi.nlm.nih.gov/31162834 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065610/ eng eng Wiley https://doi.org/10.1002/jbio.201800436 https://pubmed.ncbi.nlm.nih.gov/31162834 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065610/ © 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. J Biophotonics ISSN:1864-0648 Volume:12 Issue:9 FTIR micro spectroscopy chondroitin sulfate collagen connective tissue proteoglycans Journal Article Research Support, Non-U.S. Gov't 2019 ftpubmed https://doi.org/10.1002/jbio.201800436 2024-07-27T16:02:00Z In the present study, Fourier-transform infrared spectroscopy (FTIR) is investigated as a method to measure connective tissue components that are important for the quality of Atlantic cod filets (Gadus morhua L.). The Atlantic cod used in this study originated from a feeding trial, which found that fish fed a high starch diet contained relative more collagen type I, while fish fed a low starch (LS) diet contained relative more glycosaminoglycans (GAGs) in the connective tissue. FTIR spectra of pure commercial collagen type I and GAGs were acquired to identify spectral markers and compare them with FTIR spectra and images from connective tissue. Using principal component analysis, high and LS diets were separated due to collagen type I in the spectral region 1800 to 800 cm-1 . The spatial distribution of collagen type I and GAGs were further investigated by FTIR imaging in combination with immunohistochemistry. Pixel-wise correlation images were calculated between preprocessed connective tissue images and preprocessed pure components spectra of collagen type I and GAGs, respectively. For collagen, the FTIR images reveal a collagen distribution that closely resembles the collagen distribution as imaged by immunohistochemistry. For GAGs, the concentration is very low. Still, the FTIR images detect the most GAGs rich regions. Article in Journal/Newspaper atlantic cod Gadus morhua PubMed Central (PMC) Journal of Biophotonics 12 9 |
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Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
FTIR micro spectroscopy chondroitin sulfate collagen connective tissue proteoglycans |
spellingShingle |
FTIR micro spectroscopy chondroitin sulfate collagen connective tissue proteoglycans Sanden, Karen W Kohler, Achim Afseth, Nils K Böcker, Ulrike Rønning, Sissel B Liland, Kristian H Pedersen, Mona E The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.). |
topic_facet |
FTIR micro spectroscopy chondroitin sulfate collagen connective tissue proteoglycans |
description |
In the present study, Fourier-transform infrared spectroscopy (FTIR) is investigated as a method to measure connective tissue components that are important for the quality of Atlantic cod filets (Gadus morhua L.). The Atlantic cod used in this study originated from a feeding trial, which found that fish fed a high starch diet contained relative more collagen type I, while fish fed a low starch (LS) diet contained relative more glycosaminoglycans (GAGs) in the connective tissue. FTIR spectra of pure commercial collagen type I and GAGs were acquired to identify spectral markers and compare them with FTIR spectra and images from connective tissue. Using principal component analysis, high and LS diets were separated due to collagen type I in the spectral region 1800 to 800 cm-1 . The spatial distribution of collagen type I and GAGs were further investigated by FTIR imaging in combination with immunohistochemistry. Pixel-wise correlation images were calculated between preprocessed connective tissue images and preprocessed pure components spectra of collagen type I and GAGs, respectively. For collagen, the FTIR images reveal a collagen distribution that closely resembles the collagen distribution as imaged by immunohistochemistry. For GAGs, the concentration is very low. Still, the FTIR images detect the most GAGs rich regions. |
format |
Article in Journal/Newspaper |
author |
Sanden, Karen W Kohler, Achim Afseth, Nils K Böcker, Ulrike Rønning, Sissel B Liland, Kristian H Pedersen, Mona E |
author_facet |
Sanden, Karen W Kohler, Achim Afseth, Nils K Böcker, Ulrike Rønning, Sissel B Liland, Kristian H Pedersen, Mona E |
author_sort |
Sanden, Karen W |
title |
The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.). |
title_short |
The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.). |
title_full |
The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.). |
title_fullStr |
The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.). |
title_full_unstemmed |
The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.). |
title_sort |
use of fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of atlantic cod (gadus morhua l.). |
publisher |
Wiley |
publishDate |
2019 |
url |
https://doi.org/10.1002/jbio.201800436 https://pubmed.ncbi.nlm.nih.gov/31162834 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065610/ |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
J Biophotonics ISSN:1864-0648 Volume:12 Issue:9 |
op_relation |
https://doi.org/10.1002/jbio.201800436 https://pubmed.ncbi.nlm.nih.gov/31162834 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065610/ |
op_rights |
© 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
op_doi |
https://doi.org/10.1002/jbio.201800436 |
container_title |
Journal of Biophotonics |
container_volume |
12 |
container_issue |
9 |
_version_ |
1809898794175692800 |