Familial Alzheimer’s Disease-Related Mutations Differentially Alter Stability of Amyloid-Beta Aggregates
Amyloid-beta (Aβ) deposition as senile plaques is a pathological hallmark of Alzheimer’s disease (AD). AD is characterized by a large level of heterogeneity in amyloid pathology, whose molecular origin is poorly understood. Here, we employ NMR spectroscopy and MD simulation at ambient and high press...
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ftpubman:oai:pure.mpg.de:item_3500554 2023-08-27T04:07:51+02:00 Familial Alzheimer’s Disease-Related Mutations Differentially Alter Stability of Amyloid-Beta Aggregates Rezaei-Ghaleh, N. Amininasab, M. Giller, K. Becker, S. 2023-02-16 application/pdf http://hdl.handle.net/21.11116/0000-000C-C7DE-A http://hdl.handle.net/21.11116/0000-000C-C7E0-6 eng eng info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jpclett.2c03729 http://hdl.handle.net/21.11116/0000-000C-C7DE-A http://hdl.handle.net/21.11116/0000-000C-C7E0-6 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ The Journal of Physical Chemistry Letters info:eu-repo/semantics/article 2023 ftpubman https://doi.org/10.1021/acs.jpclett.2c03729 2023-08-02T01:43:48Z Amyloid-beta (Aβ) deposition as senile plaques is a pathological hallmark of Alzheimer’s disease (AD). AD is characterized by a large level of heterogeneity in amyloid pathology, whose molecular origin is poorly understood. Here, we employ NMR spectroscopy and MD simulation at ambient and high pressures and investigate how AD-related mutations in Aβ peptide influence the stability of Aβ aggregates. The pressure-induced monomer dissociation from Aβ aggregates monitored by NMR demonstrated that the Iowa (D23N), Arctic (E22G), and Osaka (ΔE22) mutations altered the pressure stability of Aβ40 aggregates in distinct manners. While the NMR data of monomeric Aβ40 showed only small localized effects of mutations, the MD simulation of mutated Aβ fibrils revealed their distinct susceptibility to elevated pressure. Our data propose a structural basis for the distinct stability of various Aβ fibrils and highlights “stability” as a molecular property potentially contributing to the large heterogeneity of amyloid pathology in AD. Article in Journal/Newspaper Arctic Max Planck Society: MPG.PuRe Arctic The Journal of Physical Chemistry Letters 14 6 1427 1435 |
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English |
description |
Amyloid-beta (Aβ) deposition as senile plaques is a pathological hallmark of Alzheimer’s disease (AD). AD is characterized by a large level of heterogeneity in amyloid pathology, whose molecular origin is poorly understood. Here, we employ NMR spectroscopy and MD simulation at ambient and high pressures and investigate how AD-related mutations in Aβ peptide influence the stability of Aβ aggregates. The pressure-induced monomer dissociation from Aβ aggregates monitored by NMR demonstrated that the Iowa (D23N), Arctic (E22G), and Osaka (ΔE22) mutations altered the pressure stability of Aβ40 aggregates in distinct manners. While the NMR data of monomeric Aβ40 showed only small localized effects of mutations, the MD simulation of mutated Aβ fibrils revealed their distinct susceptibility to elevated pressure. Our data propose a structural basis for the distinct stability of various Aβ fibrils and highlights “stability” as a molecular property potentially contributing to the large heterogeneity of amyloid pathology in AD. |
format |
Article in Journal/Newspaper |
author |
Rezaei-Ghaleh, N. Amininasab, M. Giller, K. Becker, S. |
spellingShingle |
Rezaei-Ghaleh, N. Amininasab, M. Giller, K. Becker, S. Familial Alzheimer’s Disease-Related Mutations Differentially Alter Stability of Amyloid-Beta Aggregates |
author_facet |
Rezaei-Ghaleh, N. Amininasab, M. Giller, K. Becker, S. |
author_sort |
Rezaei-Ghaleh, N. |
title |
Familial Alzheimer’s Disease-Related Mutations Differentially Alter Stability of Amyloid-Beta Aggregates |
title_short |
Familial Alzheimer’s Disease-Related Mutations Differentially Alter Stability of Amyloid-Beta Aggregates |
title_full |
Familial Alzheimer’s Disease-Related Mutations Differentially Alter Stability of Amyloid-Beta Aggregates |
title_fullStr |
Familial Alzheimer’s Disease-Related Mutations Differentially Alter Stability of Amyloid-Beta Aggregates |
title_full_unstemmed |
Familial Alzheimer’s Disease-Related Mutations Differentially Alter Stability of Amyloid-Beta Aggregates |
title_sort |
familial alzheimer’s disease-related mutations differentially alter stability of amyloid-beta aggregates |
publishDate |
2023 |
url |
http://hdl.handle.net/21.11116/0000-000C-C7DE-A http://hdl.handle.net/21.11116/0000-000C-C7E0-6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
The Journal of Physical Chemistry Letters |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jpclett.2c03729 http://hdl.handle.net/21.11116/0000-000C-C7DE-A http://hdl.handle.net/21.11116/0000-000C-C7E0-6 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1021/acs.jpclett.2c03729 |
container_title |
The Journal of Physical Chemistry Letters |
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14 |
container_issue |
6 |
container_start_page |
1427 |
op_container_end_page |
1435 |
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1775348568692883456 |