Biocatalytic quantification of alpha-glucan in marine particulate organic matter
Marine algae drive the marine carbon cycle, converting carbon dioxide into organic material. A major component of this produced biomass is a variety of glycans. Marine alpha-glucans include a range of storage glycans from red and green algae, bacteria, fungi, and animals. Although these compounds ar...
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ftpubman:oai:pure.mpg.de:item_3396132 2023-08-27T04:10:57+02:00 Biocatalytic quantification of alpha-glucan in marine particulate organic matter Steinke, N. Vidal-Melgosa, S. Schultz-Johansen, M. Hehemann, J. 2022-06 application/pdf http://hdl.handle.net/21.11116/0000-000A-C1A0-6 http://hdl.handle.net/21.11116/0000-000A-C1A2-4 eng eng info:eu-repo/semantics/altIdentifier/doi/10.1002/mbo3.1289 http://hdl.handle.net/21.11116/0000-000A-C1A0-6 http://hdl.handle.net/21.11116/0000-000A-C1A2-4 info:eu-repo/semantics/openAccess MICROBIOLOGYOPEN info:eu-repo/semantics/article 2022 ftpubman https://doi.org/10.1002/mbo3.1289 2023-08-02T01:09:46Z Marine algae drive the marine carbon cycle, converting carbon dioxide into organic material. A major component of this produced biomass is a variety of glycans. Marine alpha-glucans include a range of storage glycans from red and green algae, bacteria, fungi, and animals. Although these compounds are likely to account for a high amount of the carbon stored in the oceans they have not been quantified in marine samples so far. Here we present a method to extract and quantify alpha-glucans (and compare it with the beta-glucan laminarin) in particulate organic matter from algal cultures and environmental samples using sequential physicochemical extraction and enzymes as alpha-glucan-specific probes. This enzymatic assay is more specific and less susceptible to side reactions than chemical hydrolysis. Using HPAEC-PAD to detect the hydrolysis products allows for a glycan quantification in particulate marine samples down to a concentration of approximate to 2 mu g/L. We measured glucans in three cultured microalgae as well as in marine particulate organic matter from the North Sea and western North Atlantic Ocean. While the beta-glucan laminarin from diatoms and brown algae is an essential component of marine carbon turnover, our results further indicate the significant contribution of starch-like alpha-glucans to marine particulate organic matter. Henceforth, the combination of glycan-linkage-specific enzymes and chromatographic hydrolysis product detection can provide a powerful tool in the exploration of marine glycans and their role in the global carbon cycle. Article in Journal/Newspaper North Atlantic Max Planck Society: MPG.PuRe MicrobiologyOpen 11 3 |
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Max Planck Society: MPG.PuRe |
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ftpubman |
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English |
description |
Marine algae drive the marine carbon cycle, converting carbon dioxide into organic material. A major component of this produced biomass is a variety of glycans. Marine alpha-glucans include a range of storage glycans from red and green algae, bacteria, fungi, and animals. Although these compounds are likely to account for a high amount of the carbon stored in the oceans they have not been quantified in marine samples so far. Here we present a method to extract and quantify alpha-glucans (and compare it with the beta-glucan laminarin) in particulate organic matter from algal cultures and environmental samples using sequential physicochemical extraction and enzymes as alpha-glucan-specific probes. This enzymatic assay is more specific and less susceptible to side reactions than chemical hydrolysis. Using HPAEC-PAD to detect the hydrolysis products allows for a glycan quantification in particulate marine samples down to a concentration of approximate to 2 mu g/L. We measured glucans in three cultured microalgae as well as in marine particulate organic matter from the North Sea and western North Atlantic Ocean. While the beta-glucan laminarin from diatoms and brown algae is an essential component of marine carbon turnover, our results further indicate the significant contribution of starch-like alpha-glucans to marine particulate organic matter. Henceforth, the combination of glycan-linkage-specific enzymes and chromatographic hydrolysis product detection can provide a powerful tool in the exploration of marine glycans and their role in the global carbon cycle. |
format |
Article in Journal/Newspaper |
author |
Steinke, N. Vidal-Melgosa, S. Schultz-Johansen, M. Hehemann, J. |
spellingShingle |
Steinke, N. Vidal-Melgosa, S. Schultz-Johansen, M. Hehemann, J. Biocatalytic quantification of alpha-glucan in marine particulate organic matter |
author_facet |
Steinke, N. Vidal-Melgosa, S. Schultz-Johansen, M. Hehemann, J. |
author_sort |
Steinke, N. |
title |
Biocatalytic quantification of alpha-glucan in marine particulate organic matter |
title_short |
Biocatalytic quantification of alpha-glucan in marine particulate organic matter |
title_full |
Biocatalytic quantification of alpha-glucan in marine particulate organic matter |
title_fullStr |
Biocatalytic quantification of alpha-glucan in marine particulate organic matter |
title_full_unstemmed |
Biocatalytic quantification of alpha-glucan in marine particulate organic matter |
title_sort |
biocatalytic quantification of alpha-glucan in marine particulate organic matter |
publishDate |
2022 |
url |
http://hdl.handle.net/21.11116/0000-000A-C1A0-6 http://hdl.handle.net/21.11116/0000-000A-C1A2-4 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
MICROBIOLOGYOPEN |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/mbo3.1289 http://hdl.handle.net/21.11116/0000-000A-C1A0-6 http://hdl.handle.net/21.11116/0000-000A-C1A2-4 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1002/mbo3.1289 |
container_title |
MicrobiologyOpen |
container_volume |
11 |
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3 |
_version_ |
1775353352850243584 |