Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for f...
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ftpubman:oai:pure.mpg.de:item_3331451 2023-08-27T04:08:04+02:00 Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome Runge, A. Hendy, J. Richter, K. Masson-MacLean, E. Britton, K. Mackie, M. McGrath, K. Collins, M. Cappellini, E. Speller, C. 2021-07-14 application/pdf http://hdl.handle.net/21.11116/0000-0008-E37F-A http://hdl.handle.net/21.11116/0000-0008-E381-5 eng eng info:eu-repo/semantics/altIdentifier/doi/10.1098/rspb.2021.0020 http://hdl.handle.net/21.11116/0000-0008-E37F-A http://hdl.handle.net/21.11116/0000-0008-E381-5 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Proceedings of the Royal Society B: Biological Sciences info:eu-repo/semantics/article 2021 ftpubman https://doi.org/10.1098/rspb.2021.0020 2023-08-02T00:39:37Z The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. 1. Introduction 2. Material and methods (a) Samples (b) Palaeoproteomics (c) Zooarchaeology by mass spectrometry 3. Results and discussion (a) Metaproteomics (b) Host proteins (c) Dietary proteins (d) Challenges and future directions 4. Conclusion Article in Journal/Newspaper Arctic permafrost Alaska Max Planck Society: MPG.PuRe Arctic Pacific Sockeye ENVELOPE(-130.143,-130.143,54.160,54.160) Proceedings of the Royal Society B: Biological Sciences 288 1954 20210020 |
institution |
Open Polar |
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Max Planck Society: MPG.PuRe |
op_collection_id |
ftpubman |
language |
English |
description |
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. 1. Introduction 2. Material and methods (a) Samples (b) Palaeoproteomics (c) Zooarchaeology by mass spectrometry 3. Results and discussion (a) Metaproteomics (b) Host proteins (c) Dietary proteins (d) Challenges and future directions 4. Conclusion |
format |
Article in Journal/Newspaper |
author |
Runge, A. Hendy, J. Richter, K. Masson-MacLean, E. Britton, K. Mackie, M. McGrath, K. Collins, M. Cappellini, E. Speller, C. |
spellingShingle |
Runge, A. Hendy, J. Richter, K. Masson-MacLean, E. Britton, K. Mackie, M. McGrath, K. Collins, M. Cappellini, E. Speller, C. Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
author_facet |
Runge, A. Hendy, J. Richter, K. Masson-MacLean, E. Britton, K. Mackie, M. McGrath, K. Collins, M. Cappellini, E. Speller, C. |
author_sort |
Runge, A. |
title |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_short |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_full |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_fullStr |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_full_unstemmed |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_sort |
palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
publishDate |
2021 |
url |
http://hdl.handle.net/21.11116/0000-0008-E37F-A http://hdl.handle.net/21.11116/0000-0008-E381-5 |
long_lat |
ENVELOPE(-130.143,-130.143,54.160,54.160) |
geographic |
Arctic Pacific Sockeye |
geographic_facet |
Arctic Pacific Sockeye |
genre |
Arctic permafrost Alaska |
genre_facet |
Arctic permafrost Alaska |
op_source |
Proceedings of the Royal Society B: Biological Sciences |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1098/rspb.2021.0020 http://hdl.handle.net/21.11116/0000-0008-E37F-A http://hdl.handle.net/21.11116/0000-0008-E381-5 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1098/rspb.2021.0020 |
container_title |
Proceedings of the Royal Society B: Biological Sciences |
container_volume |
288 |
container_issue |
1954 |
container_start_page |
20210020 |
_version_ |
1775348775258161152 |