Methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum
Two isolates of aerobic methanotrophic bacteria, strains Sph1T and Sph2, were obtained from cold methane seeps in a floodplain of the river Mukhrinskaya, Irtysh basin, West Siberia. Another morphologically and phenotypically similar methanotroph, strain OZ2, was isolated from a sediment of a subarct...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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ftpubman:oai:pure.mpg.de:item_3271327 2023-08-27T04:06:24+02:00 Methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum Oshkin, I. Belova, S. Danilova, O. Miroshnikov, K. Rijpstra, W. Damste, J. Liesack, W. Dedysh, S. 2016-06 http://hdl.handle.net/21.11116/0000-0007-BBF7-0 unknown info:eu-repo/semantics/altIdentifier/doi/10.1099/ijsem.0.001046 http://hdl.handle.net/21.11116/0000-0007-BBF7-0 International Journal of Systematic and Evolutionary Microbiology info:eu-repo/semantics/article 2016 ftpubman https://doi.org/10.1099/ijsem.0.001046 2023-08-02T02:00:21Z Two isolates of aerobic methanotrophic bacteria, strains Sph1T and Sph2, were obtained from cold methane seeps in a floodplain of the river Mukhrinskaya, Irtysh basin, West Siberia. Another morphologically and phenotypically similar methanotroph, strain OZ2, was isolated from a sediment of a subarctic freshwater lake, Archangelsk region, northern Russia. Cells of these three strains were Gram-stain-negative, light-pink-pigmented, non-motile, encapsulated, large cocci that contained an intracytoplasmic membrane system typical of type I methanotrophs. They possessed a particulate methane monooxygenase enzyme and utilized only methane and methanol. Strains Sph1T, Sph2 and OZ2 were able to grow at a pH range of 4.0–8.9 (optimum at pH 6.0–7.0) and at temperatures between 2 and 36 °C. Although their temperature optimum was at 20–25 °C, these methanotrophs grew well at lower temperatures, down to 4 °C. The major cellular fatty acids were C16 : 1ω5c, C16 : 1ω6c, C16 : 1ω7c, C16 : 1ω8c, C16 : 0 and C14 : 0; the DNA G+C content was 51.4–51.9 mol%. Strains Sph1T, Sph2 and OZ2 displayed nearly identical (99.1–99.7 % similarity) 16S rRNA gene sequences and belonged to the family Methylococcaceae of the class Gammaproteobacteria . The most closely related organism was Methylovulum miyakonense HT12T (96.0–96.5 % 16S rRNA gene sequence similarity and 90 % pmoA sequence similarity). The novel isolates, however, differed from Methylovulum miyakonense HT12T by cell morphology, pigmentation, absence of soluble methane monooxygenase, more active growth at low temperatures, growth over a broader pH range and higher DNA G+C content. On the basis of these differences, we propose a novel species, Methylovulum psychrotolerans sp. nov., to accommodate these methanotrophs. Strain Sph1T (=LMG 29227T=VKM B-3018T) is the type strain. Article in Journal/Newspaper Archangelsk Subarctic Siberia Max Planck Society: MPG.PuRe International Journal of Systematic and Evolutionary Microbiology 66 6 2417 2423 |
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Max Planck Society: MPG.PuRe |
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Two isolates of aerobic methanotrophic bacteria, strains Sph1T and Sph2, were obtained from cold methane seeps in a floodplain of the river Mukhrinskaya, Irtysh basin, West Siberia. Another morphologically and phenotypically similar methanotroph, strain OZ2, was isolated from a sediment of a subarctic freshwater lake, Archangelsk region, northern Russia. Cells of these three strains were Gram-stain-negative, light-pink-pigmented, non-motile, encapsulated, large cocci that contained an intracytoplasmic membrane system typical of type I methanotrophs. They possessed a particulate methane monooxygenase enzyme and utilized only methane and methanol. Strains Sph1T, Sph2 and OZ2 were able to grow at a pH range of 4.0–8.9 (optimum at pH 6.0–7.0) and at temperatures between 2 and 36 °C. Although their temperature optimum was at 20–25 °C, these methanotrophs grew well at lower temperatures, down to 4 °C. The major cellular fatty acids were C16 : 1ω5c, C16 : 1ω6c, C16 : 1ω7c, C16 : 1ω8c, C16 : 0 and C14 : 0; the DNA G+C content was 51.4–51.9 mol%. Strains Sph1T, Sph2 and OZ2 displayed nearly identical (99.1–99.7 % similarity) 16S rRNA gene sequences and belonged to the family Methylococcaceae of the class Gammaproteobacteria . The most closely related organism was Methylovulum miyakonense HT12T (96.0–96.5 % 16S rRNA gene sequence similarity and 90 % pmoA sequence similarity). The novel isolates, however, differed from Methylovulum miyakonense HT12T by cell morphology, pigmentation, absence of soluble methane monooxygenase, more active growth at low temperatures, growth over a broader pH range and higher DNA G+C content. On the basis of these differences, we propose a novel species, Methylovulum psychrotolerans sp. nov., to accommodate these methanotrophs. Strain Sph1T (=LMG 29227T=VKM B-3018T) is the type strain. |
format |
Article in Journal/Newspaper |
author |
Oshkin, I. Belova, S. Danilova, O. Miroshnikov, K. Rijpstra, W. Damste, J. Liesack, W. Dedysh, S. |
spellingShingle |
Oshkin, I. Belova, S. Danilova, O. Miroshnikov, K. Rijpstra, W. Damste, J. Liesack, W. Dedysh, S. Methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum |
author_facet |
Oshkin, I. Belova, S. Danilova, O. Miroshnikov, K. Rijpstra, W. Damste, J. Liesack, W. Dedysh, S. |
author_sort |
Oshkin, I. |
title |
Methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum |
title_short |
Methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum |
title_full |
Methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum |
title_fullStr |
Methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum |
title_full_unstemmed |
Methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus Methylovulum |
title_sort |
methylovulum psychrotolerans sp nov:, a cold-adapted methanotroph from low-temperature terrestrial environments, and emended description of the genus methylovulum |
publishDate |
2016 |
url |
http://hdl.handle.net/21.11116/0000-0007-BBF7-0 |
genre |
Archangelsk Subarctic Siberia |
genre_facet |
Archangelsk Subarctic Siberia |
op_source |
International Journal of Systematic and Evolutionary Microbiology |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1099/ijsem.0.001046 http://hdl.handle.net/21.11116/0000-0007-BBF7-0 |
op_doi |
https://doi.org/10.1099/ijsem.0.001046 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
66 |
container_issue |
6 |
container_start_page |
2417 |
op_container_end_page |
2423 |
_version_ |
1775347326671388672 |