Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments
Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the delta subclass of Proteobacteria. and were widely distributed within this group, indicating that psychr...
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ftpubman:oai:pure.mpg.de:item_3154143 2023-08-27T04:07:34+02:00 Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments Sahm, K. Knoblauch, C. Amann, R. 1999 application/pdf http://hdl.handle.net/21.11116/0000-0005-4EA5-A http://hdl.handle.net/21.11116/0000-0005-4EA7-8 eng eng http://hdl.handle.net/21.11116/0000-0005-4EA5-A http://hdl.handle.net/21.11116/0000-0005-4EA7-8 info:eu-repo/semantics/openAccess Applied and Environmental Microbiology info:eu-repo/semantics/article 1999 ftpubman 2023-08-02T00:10:20Z Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the delta subclass of Proteobacteria. and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes,was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing :bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3.5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments. Article in Journal/Newspaper Arctic Hornsund Svalbard Spitsbergen Max Planck Society: MPG.PuRe Arctic Arctic Island ENVELOPE(-74.766,-74.766,62.234,62.234) Hornsund ENVELOPE(15.865,15.865,76.979,76.979) Svalbard |
institution |
Open Polar |
collection |
Max Planck Society: MPG.PuRe |
op_collection_id |
ftpubman |
language |
English |
description |
Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the delta subclass of Proteobacteria. and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes,was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing :bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3.5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments. |
format |
Article in Journal/Newspaper |
author |
Sahm, K. Knoblauch, C. Amann, R. |
spellingShingle |
Sahm, K. Knoblauch, C. Amann, R. Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments |
author_facet |
Sahm, K. Knoblauch, C. Amann, R. |
author_sort |
Sahm, K. |
title |
Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments |
title_short |
Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments |
title_full |
Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments |
title_fullStr |
Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments |
title_full_unstemmed |
Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments |
title_sort |
phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine arctic sediments |
publishDate |
1999 |
url |
http://hdl.handle.net/21.11116/0000-0005-4EA5-A http://hdl.handle.net/21.11116/0000-0005-4EA7-8 |
long_lat |
ENVELOPE(-74.766,-74.766,62.234,62.234) ENVELOPE(15.865,15.865,76.979,76.979) |
geographic |
Arctic Arctic Island Hornsund Svalbard |
geographic_facet |
Arctic Arctic Island Hornsund Svalbard |
genre |
Arctic Hornsund Svalbard Spitsbergen |
genre_facet |
Arctic Hornsund Svalbard Spitsbergen |
op_source |
Applied and Environmental Microbiology |
op_relation |
http://hdl.handle.net/21.11116/0000-0005-4EA5-A http://hdl.handle.net/21.11116/0000-0005-4EA7-8 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1775348333773062144 |