Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP)
Abstract Many bird species are sexually monomorphic and cannot be sexed based on phenotypic traits. Rapid sex determination is often a necessary component of avian studies focusing on behavior, ecology, evolution, and conservation. While PCR-based methods are the most common technique for molecularl...
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ftpubman:oai:pure.mpg.de:item_3074233 2023-08-27T04:08:34+02:00 Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP) Koch, H. Blohm-Sievers, E. Liedvogel, M. 2019-05 application/pdf http://hdl.handle.net/21.11116/0000-0004-472B-D http://hdl.handle.net/21.11116/0000-0004-472D-B eng eng info:eu-repo/semantics/altIdentifier/doi/10.1002/ece3.5168 http://hdl.handle.net/21.11116/0000-0004-472B-D http://hdl.handle.net/21.11116/0000-0004-472D-B info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ Ecology and Evolution info:eu-repo/semantics/article 2019 ftpubman https://doi.org/10.1002/ece3.5168 2023-08-02T00:01:25Z Abstract Many bird species are sexually monomorphic and cannot be sexed based on phenotypic traits. Rapid sex determination is often a necessary component of avian studies focusing on behavior, ecology, evolution, and conservation. While PCR-based methods are the most common technique for molecularly sexing birds in the laboratory, a simpler, faster, and cheaper method has emerged, which can be used in the laboratory, but importantly also in the field. Herein, we used loop-mediated isothermal amplification (LAMP) for rapid sex determination of blood samples from juvenile European blackcaps, Sylvia atricapilla, sampled in the wild. We designed LAMP primers unique to S. atricapilla based on the sex chromosome-specific gene, chromo-helicase-DNA-binding protein (CHD), optimized the primers for laboratory and field application, and then used them to test a subset of wild-caught juvenile blackcaps of unknown gender at the time of capture. Sex determination results were fast and accurate. The advantages of this technique are that it allows researchers to identify the sex of individual birds within hours of sampling and eliminates the need for direct access to a laboratory if implemented at a remote field site. This work adds to the increasing list of available LAMP primers for different bird species and is a new addition within the Passeriformes order. Article in Journal/Newspaper Avian Studies Max Planck Society: MPG.PuRe Ecology and Evolution 9 10 5849 5858 |
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Max Planck Society: MPG.PuRe |
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ftpubman |
language |
English |
description |
Abstract Many bird species are sexually monomorphic and cannot be sexed based on phenotypic traits. Rapid sex determination is often a necessary component of avian studies focusing on behavior, ecology, evolution, and conservation. While PCR-based methods are the most common technique for molecularly sexing birds in the laboratory, a simpler, faster, and cheaper method has emerged, which can be used in the laboratory, but importantly also in the field. Herein, we used loop-mediated isothermal amplification (LAMP) for rapid sex determination of blood samples from juvenile European blackcaps, Sylvia atricapilla, sampled in the wild. We designed LAMP primers unique to S. atricapilla based on the sex chromosome-specific gene, chromo-helicase-DNA-binding protein (CHD), optimized the primers for laboratory and field application, and then used them to test a subset of wild-caught juvenile blackcaps of unknown gender at the time of capture. Sex determination results were fast and accurate. The advantages of this technique are that it allows researchers to identify the sex of individual birds within hours of sampling and eliminates the need for direct access to a laboratory if implemented at a remote field site. This work adds to the increasing list of available LAMP primers for different bird species and is a new addition within the Passeriformes order. |
format |
Article in Journal/Newspaper |
author |
Koch, H. Blohm-Sievers, E. Liedvogel, M. |
spellingShingle |
Koch, H. Blohm-Sievers, E. Liedvogel, M. Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP) |
author_facet |
Koch, H. Blohm-Sievers, E. Liedvogel, M. |
author_sort |
Koch, H. |
title |
Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP) |
title_short |
Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP) |
title_full |
Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP) |
title_fullStr |
Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP) |
title_full_unstemmed |
Rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (LAMP) |
title_sort |
rapid sex determination of a wild passerine species using loop-mediated isothermal amplification (lamp) |
publishDate |
2019 |
url |
http://hdl.handle.net/21.11116/0000-0004-472B-D http://hdl.handle.net/21.11116/0000-0004-472D-B |
genre |
Avian Studies |
genre_facet |
Avian Studies |
op_source |
Ecology and Evolution |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/ece3.5168 http://hdl.handle.net/21.11116/0000-0004-472B-D http://hdl.handle.net/21.11116/0000-0004-472D-B |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/ece3.5168 |
container_title |
Ecology and Evolution |
container_volume |
9 |
container_issue |
10 |
container_start_page |
5849 |
op_container_end_page |
5858 |
_version_ |
1775349407188779008 |